A fast method for the identification of Mycobacterium tuberculosis in sputum and cultures based on thermally assisted hydrolysis and methylation followed by gas chromatography–mass spectrometry

Recent applications in analytical thermochemolysis

“…PAH quinines were reacted with cysteine and the resulting thiol and amine adducts were identified by thermochemolysis. Kaal et al [173] applied thermochemolysis-TMAH in the diagnosis of tuberculosis. Sputum samples were characterized by identification of two markers, tuberculostearic acid (TBSA) and hexacosanoic acid.…”

Proton Transfer Reaction Mass Spectrometry: Applications in the Life Sciences

“…In the literature, some data has been gathered using electronic nose (enose) measurements ( [175]; [42]), but this method does not provide information about the identity of the volatiles. Gas chromatographic (GC) methods have been successfully employed previously for mycobacterial identification purposes ( [165]; [176]; [177]), but such methods require sample manipulation and preparation, due to their lack of sensitivity as compared to PTR-MS, resulting in a "hands on" assay and higher risk of contamination.…”

Direct detection of Mycobacterium tuberculosis in sputum: A validation study using solid phase extraction-gas chromatography–mass spectrometry

“…However, we discovered that these markers were not specific enough because they are not only present in MTB but also in other mycobacteria [17,18].…”

“…Originally we used a combination of two biomarkers, tuberculostearic acid (TBSA) and hexacosanoic acid (C26) [17]. However, we discovered that these markers were not specific enough because they are not only present in MTB but also in other mycobacteria [17,18].…”

“…In 2009, we developed a fully automated method for the identification of cultured MTB and for the detection of MTB directly in sputum [17]. The method uses thermally-assisted 5 hydrolysis and methylation gas chromatography-mass spectrometry (THM-GC-MS).…”