The use of the thermostatic metal bath is becoming more and more extensive and the requirements for its precision and reliability are also increasing. To meet the needs of the metal bath calibration, a 12-channel thermostatic metal bath temperature field calibration system based on a four-wire PT100 has been designed. The system design includes a front-end temperature measurement component, which consists of a four-wire PT100 and a thermostatic block, and a signal processing component, which consists of a bidirectional constant current source excitation unit, a signal conditioning unit and a high-precision acquisition unit. The STM32f407 is used as the main control chip, and the analog channel selector is used for 12-channel selection. The constant current source is used for signal excitation, the proportional method is used to measure the PT100 resistance, and an acquisition circuit with a high-precision 32-bit ADS1263 analog-to-digital converter is used to amplify, filter and convert the analog signal. After piecewise linear fitting and system calibration, the temperature measurement accuracy can reach 0.4 mK, which meets the calibration requirements of the thermostatic metal bath.