A Fast-Response AIE-Active Ratiometric Fluorescent Probe for the Detection of Carboxylesterase

Abstract

Hepatocellular carcinoma (HCC) is associated with a high mortality rate worldwide. The therapeutic outcomes can be significantly improved if diagnosis and treatment are initiated earlier in the disease process. Recently, the carboxylesterase (CaE) activity/level in human plasma was reported to be a novel serological biomarker candidate for HCC. In this article, we fabricated a new fluorescent probe with AIE characteristics for the rapid detection of CaE with a more reliable ratiometric response mode. The TCFISE probe showed high sensitivity (LOD: 93.0 μU/mL) and selectivity toward CaE. Furthermore, the good pH stability, superior resistance against photobleaching, and low cytotoxicity highlight the high potential of the TCFISE probe for application in the monitoring of CaE activity in complex biological samples and in live cells, tissues, and animals.

A Reversible Mechanochromic AIEgen Based on Triphenylamine for the Selective Detection of Vitamin B2 (Riboflavin) in Aqueous Media and Histotoxicity

“…This process is dependent on the amount of water present in the mixture with increasing water fractions leading to greater aggregate formation. These remarkable fluorescent luminogens, known as AIEgens, have found widespread use in various fields. , …”

Multifunctional Fluorescent Tetraphenylethene-Based Reversible Mechanochromism for Highly Selective Detection of MnO4– in Aqueous Media and Green Organic Light Emitting Diode Applications

“…It is directly linked to the aggregation of luminogens and their fluorescence emission in an aggregate (solid) state. − In the aggregation-induced emission process, the nonemissive or weakly emissive luminogens are converted into highly emissive because of the aggregate formation of the luminogens after increasing the fractions of water, which depends on the water quantity in the mixture. Such types of remarkable fluorescent luminogens or AIEgens have been widely used in diverse fields. , …”

“…Such types of remarkable fluorescent luminogens or AIEgens have been widely used in diverse fields. 33,42 Accordingly, to verify the (AIE phenomenon, we have performed this activity by using THF (tetrahydrofuran) and water. The photographic images under daylight and UV light are depicted in (Figure 1a).…”

A ratiometric nanoprobe for the in vivo bioimaging of hypochlorous acid to detect drug-damaged liver and kidneys

“…37,38 Near-infrared sensors have been developed rapidly due to their characteristics of causing low damage, low background fluorescence, and deep tissue penetration. 39–54…”

“…37,38 Near-infrared sensors have been developed rapidly due to their characteristics of causing low damage, low background fluorescence, and deep tissue penetration. [39][40][41][42][43][44][45][46][47][48][49][50][51][52][53][54] Herein, we designed a near-infrared probe NRH-800 (Scheme 1), which uses rhodamine derivatives as a fluorescent nuclear component to modify the positively charged indole rings that tend to be enriched in mitochondria and the transdouble bond of probe molecules, which can be cut by HClO to trigger fluorescence changes. NRH-800 can thus meet the demands of imaging in the near-infrared fluorescence mode and has a sufficiently large emission shift.…”