A fast-responsive fluorescent turn-on probe for nitroreductase imaging in living cells

Jia, Chengli; Zhang, Yong; Wang, Yuesong; Ji, Min

doi:10.1039/d0ra09512e

Cited by 9 publications

(4 citation statements)

References 34 publications

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“…However, further research and validation may be necessary before widespread adoption in clinical or research applications. 64…”

Section: Phase I Xenobiotic-metabolizing Enzymesmentioning

confidence: 99%

Real-time fluorescent monitoring of phase I xenobiotic-metabolizing enzymes

Iqbal,

Ilyas,

Akash

et al. 2024

RSC Adv.

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“…However, further research and validation may be necessary before widespread adoption in clinical or research applications. 64…”

Section: Phase I Xenobiotic-metabolizing Enzymesmentioning

confidence: 99%

Real-time fluorescent monitoring of phase I xenobiotic-metabolizing enzymes

Iqbal,

Ilyas,

Akash

et al. 2024

RSC Adv.

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“…The HeLa cells when cultured with probe 8 under normoxic (20 % pO2) and hypoxic (1 % pO2) environments, the hypoxic condition produced significantly more fluorescence intensity than the normoxic state indicating its potential of monitoring NTR in tumor cells. [48] A Tumor-targeting component called folate was included in cancer cell-selective, hypoxia-responsive (Near-infrared) Probe 9 (Chart 4), which targets folate receptor-positive CT26 cancer cells rather than folate receptor-negative cell lines WI38. When CT26 cells and WI38 cells were subjected to identical experimental conditions, their CLSM (confocal laser scanning microscopy) and FACS (fluorescence-activated cell sorting) analyses using the probe revealed a lower fluorescence intensity in the case of WI38 cells than in CT26 cells.…”

Section: Development Of Fluorescent Probes For Nitroreductase Detecti...mentioning

confidence: 99%

“…Some of the most recent developments in fluorescence probes for the detection of NTR in animal cells are listed here; Probe 8 (Chart 4) possessing a basic skeleton of fused four‐ring quinoxaline, successfully monitored the endogenous NTR in HeLa cells. The HeLa cells when cultured with probe 8 under normoxic (20 % pO2) and hypoxic (1 % pO2) environments, the hypoxic condition produced significantly more fluorescence intensity than the normoxic state indicating its potential of monitoring NTR in tumor cells [48] …”

Section: Nitroreductases In Animal Cellsmentioning

confidence: 99%

Exploring Multifaceted Roles of Nitroreductases in Bacterial, Animal and Plant Biosystems and their Detection using Fluorescent Probes

Verma,

Singla,

Bhadwal

et al. 2024

ChemistrySelect

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Nitroreductases are the enzymes that can convert nitroaromatic substances including explosives like TNT to their corresponding nitrite, hydroxylamine or amino derivatives with reduced NADH or NADPH as an electron donor. A wide range of nitroreductases are known to present in bacterial genomes that are explored for their potential in the reduction of nitroaromatic compounds and they are used in converting prodrugs to toxic hydroxylamine derivatives causing tumor cell killing. Likewise, in cancer cells, the low oxygen condition called hypoxia is a characteristic of solid tumors and during hypoxia nitroreductase gene expression occurs. Moreover, due to the ability of nitroreductases to reduce nitroaromatic compounds, phytoremediation potential of plants was inspected by expressing bacterial nitroreductase genes in plants through genetic engineering. Due to its diversity and widespread applications in different biosystems, its detection has gained momentum among the scientific community. Designing fluorescent probes for detecting nitroreductases has emerged as a beneficial tool compared to conventional monitoring approaches due to their high sensitivity, selectivity, and real‐time monitoring ability. This review aims to provide an understanding of the activity and the status of nitroreductases in bacteria, plants and animal cells as well as the significance of fluorescent imaging‐related studies by employing recently designed probes.

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“…Jia et al . synthesized the fused four‐ring quinoxaline turn‐on probe 41 for detection of NTR [78] . Probe 41 showed fluorescence emission wavelength at 541 nm due to subsequent reduction to compound 41 a with a 30‐fold increase in fluorescence intensity (Figure 39).…”

Section: Small Molecule Based Ntr Probesmentioning

confidence: 99%

Small Molecule Fluorescent Probes for Sensing and Bioimaging of Nitroreductase

et al. 2022

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Hypoxia is a condition caused by a decrease in oxygen and is often seen in cases of cardiac ischemia, solid tumors, inflammation, and other diseases. Tumor hypoxia can result in cancer invasion, metastasis, decreased apoptosis, and resistance to various therapies (chemotherapy, immunotherapy, and radiotherapy), ultimately reducing overall survival rates. These effects of hypoxia underline the need to monitor hypoxia‐associated specific markers for more effective therapeutic intervention. Nitroreductase (NTR) is a common biomarker of hypoxia, via its reduction activity with nitroaromatic compounds to corresponding amino derivatives using NADH and NADPH as cofactor. The NTR expression level can be correlated to cellular hypoxia. Therefore, developing a diagnostic tool for measuring NTR activity is of immense importance for improving treatment efficacies. To leverage NTR activity as a proxy of hypoxia, small molecule based fluorescent probes have been developed as quantifiable probes. To date, several NTR‐sensitive fluorescent probes have been reported. This review is focuses on the advances in these fluorescent NTR probes over the last five years.

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A fast-responsive fluorescent turn-on probe for nitroreductase imaging in living cells

Abstract: Probe NTR-NO2 was effectively reduced in the presence of NTR generating a highly fluorescent product.

Cited by 9 publications

References 34 publications

Real-time fluorescent monitoring of phase I xenobiotic-metabolizing enzymes

Real-time fluorescent monitoring of phase I xenobiotic-metabolizing enzymes

Exploring Multifaceted Roles of Nitroreductases in Bacterial, Animal and Plant Biosystems and their Detection using Fluorescent Probes

Small Molecule Fluorescent Probes for Sensing and Bioimaging of Nitroreductase

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