A Fatty Acid Fraction Purified From Sea Buckthorn Seed Oil Has Regenerative Properties on Normal Skin Cells

Abstract

In recent years, natural product's research gained momentum, fueled by technological advancement and open availability of research data. To date, sea buckthorn (Hippophae rhamnoides L. [Elaeagnaceae]) plant parts, especially berries, are well characterized and repeatedly tested for antioxidant activity and regenerative properties, in various cell types and tissues. However, fatty acids (FA) have been less investigated in term of biological effects, although, they are important bioactive components of the sea buckthorn fruit and oil. The aim of our work was to determine whether sea buckthorn seed oil is a suitable source of FA with regenerative properties on normal skin cells. Using high-performance liquid chromatography (HPLC) and liquid chromatography – mass spectrometry (LC-MS), we purified and characterized four fractions enriched in saturated (palmitic) and non-saturated (linoleic, alfa-linolenic, oleic) FA, which were tested for cytotoxicity, cytokine and growth factor production, and regenerative effect on normal keratinocytes and skin fibroblasts. Evidence is presented that the palmitic acid enriched fraction was a suitable sea buckthorn seed oil derived product with cell proliferation properties on both skin cell types.

Preparation, Characterization and Biological Activities of an Oil-in-Water Nanoemulsion from Fish By-Products and Lemon Oil by Ultrasonication Method

“…Fatty acids from fish oil might also be one of the reasons for skin cell proliferation. The fatty acids, such as palmitic acid, aided in keratinocytes and fibroblast proliferation, possibly by additionally fuelling cellular metabolism [ 56 ]. A review by Carvalho et al [ 57 ] shows that omega-6 fatty acids have received much attention because they can modulate cell migration and proliferation, phagocytic capacity, and synthesis of inflammatory mediators, which are also found in the fish oil used in the NE-FLO.…”

Research Status and Development Prospects of Sea buckthorn (Hippophae rhamnoides L.) Resources in China

“…The wound-healing potential of Sea buckthorn seed oil depends on the presence of ω-3 and ω-6 fatty acids, tocopherols, and carotenoids [88]. Additionally, a fraction enriched with palmitic acid purified from Sea buckthorn seed oil exhibited cellproliferative properties by promoting the growth of keratinocytes and dermal fibroblasts, making it suitable for developing skincare formulations and products [89]. Moreover, Sea buckthorn oil has been tested for its potential to alleviate menopausal symptoms such as vaginal atrophy and thinning and drying of the vaginal mucosa.…”

Phytochemistry, health benefits, and food applications of sea buckthorn (Hippophae rhamnoides L.): A comprehensive review

“…The wound healing potential of sea buckthorn seed oil is dependent on the presence of omega-3 and omega-6 fatty acids, tocopherols and carotenoids ( 75 ). In addition, the palmitic acid-rich fraction purified from sea buckthorn seed oil has cell proliferation properties that promote growth of keratinocytes and dermal fibroblasts, which can be used to develop skin preparations and skin care products ( 76 ). UV light induces damage to the redox system and impairs lipid metabolism in skin fibroblasts and keratin-forming cells, and sea buckthorn oil can inhibit this effect and sea buckthorn seed oil may be a promising natural substance for skin photoprotection ( 55 ).…”