A feasibility study evaluating an <i>in situ</i> formed synthetic biodegradable membrane for guided bone regeneration in dogs

Jung, Ronald E.; Lecloux, Geoffrey; Rompen, Eric; Ramel, Christian; Buser, Daniel; Hämmerle, Christoph H.F.

doi:10.1111/j.1600-0501.2008.01633.x

Cited by 50 publications

(71 citation statements)

References 35 publications

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“…For our investigation we chose two well evaluated materials, mPEG hydrogels and DBBM. Both materials have previously been clinically tested for bone augmentation procedures in dentistry [7,9,[25][26][27][28][29][30]32].…”

Section: Biological Activity Of Rhbmp-2 -Impact Of Bone Substitute Mamentioning

confidence: 99%

“…An approach for a system composed of clinically evaluated materials that provides mechanical stability and allows sustained growth factor delivery is the combination of DBBM with hydrolysable polyethylene glycol (PEG) hydrogels [25][26][27][28][29][30]. In such a system, the release profile of the growth factor is dependent on the physical binding strength to the hydrogel, the degradation characteristics of the hydrogel and the interaction of the applied growth factor with the DBBM.…”

Section: Introductionmentioning

confidence: 99%

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Analysis of hydrolyzable polyethylene glycol hydrogels and deproteinized bone mineral as delivery systems for glycosylated and non-glycosylated bone morphogenetic protein-2

Hänseler

Jung

et al. 2012

Acta Biomaterialia

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Bone morphogenetic proteins (BMP), in particular BMP-2, are the growth factors primarily responsible for osteoinduction. A knowledge of interactions between bone substitute materials and growth factor variants is crucial to designing bone substitutes with an ideal release profile. Here we compare glycosylated and non-glycosylated recombinant human bone morphogenetic protein-2 (rhBMP-2) either incorporated into a hydrolyzable polyethylene glycol (PEG) hydrogel developed as a slow release system or adsorbed to a deproteinized bovine bone matrix (DBBM), a clinically well-established bone substitute material. rhBMP-2 loaded materials were immersed in cell culture medium and rhBMP-2 concentration profiles in the supernatant were determined by an enzyme-linked immunosorbent assay. The corresponding biological activities were assessed in vitro by alkaline phosphatase activity assay. We show a strong affinity of rhBMP-2 for DBBM and reduced biological activity after its release from PEG hydrogels. Glycosylated rhBMP-2 was significantly less affected by the hydrogel and interacted significantly more strongly with DBBM than non-glycosylated rhBMP-2. We therefore question the combination of PEG hydrogels with DBBM as a rhBMP-2 delivery system over DBBM alone, since rhBMP-2 released from the hydrogel will be trapped by DBBM. Moreover, our results suggest that glycosylated rhBMP-2 is favorable in combination with PEG hydrogels, since its activity is better preserved, whereas in combination with DBBM non-glycosylated rhBMP-2 is favorable, benefiting from an initially higher concentration of free rhBMP-2. The corresponding biological activities were assessed in vitro by an alkaline phosphatase activity assay. We show a strong affinity of rhBMP-2 to DBBM and a reduced biological activity after its release from PEG hydrogels. Glycosylated rhBMP-2 was significantly less affected by hydrogels and interacted significantly stronger with DBBM than non-glycosylated rhBMP-2. We therefore question the combination of PEG hydrogels with DBBM as a rhBMP-2 delivery system over DBBM alone since rhBMP-2 released from the hydrogel will be trapped by DBBM.Moreover, our results suggest that glycosylated rhBMP-2 is favorable in combination with PEG hydrogels since its activity is better preserved. Whereas in combination with DBBM, non-glycosylated rhBMP-2 is favorable in order to benefit from an initially higher concentration of free rhBMP-2.

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Section: Biological Activity Of Rhbmp-2 -Impact Of Bone Substitute Mamentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Analysis of hydrolyzable polyethylene glycol hydrogels and deproteinized bone mineral as delivery systems for glycosylated and non-glycosylated bone morphogenetic protein-2

Hänseler

Jung

et al. 2012

Acta Biomaterialia

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“…The surface structure of the modified materials (toughness R a > 0.5 µm) can stimulate osteoinductivity, promote bone cell adhesion and proliferation, regulate the release and enrichment of bone morphogenetic protein-2/transforming growth factor-β (BMP-2/TGF-β) [10], induce bone marrow stem cells to differentiate into osteoblasts [11], and reduce osteoclast activity [12]. Types of implant materials with surface osteoinductivity have been developed quickly.…”

Section: Introductionmentioning

confidence: 99%

Surface Properties and MC3T3-E1 Cell Response of Cortical Bone Allografts Modified with Low-Concentration Phosphoric Acid

Huang

Cheng

Wang

et al. 2017

Cell Physiol Biochem

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Background/Aims: This experimental study aimed to evaluate the effect of low-concentration phosphoric acid on the surface structure of cortical allografts. Methods: Allogenic cortical bones were obtained from femurs and tibias of New Zealand white rabbits. The bones were modified by treatment with various concentrations of phosphoric acid (10%, 20% or 30%) for 10, 30 or 60 minutes, then evaluated by the following methods: 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) and LIVE/DEAD assay, alkaline phosphatase (ALP) assay, biomechanical properties testing, contact angle detection, quantitative real-time polymerase chain reaction (Q-PCR), western blotting and scanning electron microscopy (SEM). Results: Compared with the other groups, the group modified with 10% H₃PO₄ for 10 minutes had lower cytotoxicity according to MTT and LIVE/DEAD assays, higher hydrophilicity in the contact angle detection test and greater stability in the biomechanical properties test. Moreover, an up-regulation of osteopontin (OPN) in bones modified with 10% H₃PO₄ was observed by Q-PCR and western blotting. In addition, ALP assay and SEM showed that surface porosity and osteoinductivity were increased in the group modified with 10% H₃PO₄. Conclusions: Low-concentration phosphoric acid may be a potential method for surface modification of cortical allografts. Further animal experiments and animal infection model studies are required to validate the efficacy of surface-modified cortical allografts to repair large segmental bone defects.

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“…Several animal studies have demonstrated that collagen membrane has the capacity to support regeneration of periodontal tissues and is either incorporated within the healing tissues or degraded by these during the healing process [3,5,15]. These findings suggest that collagen membranes may be of value in reconstructive periodontal therapy.…”

Section: Resultsmentioning

confidence: 98%

“…The probing depth in this study was decreased with a ratio of 50%. Such significant pocket depth reduction is important, because this parameter serves as a diagnostic test with high sensitivity and specificity [14][15][16]. Aukhil et al also found that placement of physical barriers between the root surface and flaps may be beneficial in facilitating coronal migration of progenitor cells from the periodontal ligament [1].…”

Section: Resultsmentioning

confidence: 99%

A novel porcine collagen GTR membrane for treatment of Class II molar furcation involvement

Hsu

Lin

Chang³

et al. 2014

Journal of Polymer Engineering

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Guided tissue regeneration (GTR) in the management of the periodontal furcation invasion is effective. Recent studies showed significant clinical improvement with different materials. The purpose of this study is to provide clinical evaluations of the bone repair potential of GTR combined with a novel porcine collagen membrane in the treatment of human molar Class II furcations. Fifty-one patients, 33 males and 18 females, with no systemic disease and a mean age of 49.1 years, participated in this study after phase I periodontal therapy. Probing depth, attachment loss and radiographic examination were recorded and analyzed before and after GTR therapy with collagen membrane. In the soft tissue measurement, the periodontal pocket (PD) depth was 5.283 ± 0.283 mm before the surgery and improved to 2.631 ± 0.147 mm at 9 months after surgery (p < 0.05). Attachment loss also showed similar results. The attachment level was 5.547 ± 0.3 mm before the surgery, and 2.911 ± 0.188 mm at 9 months after surgery (p < 0.05). According to the measurements the attachment gain was 2.636 ± 0.168 mm after GTR surgery with porcine collagen membrane. According to this study, the porcine collagen membrane can perform with exceptional results in attachment level gain for the Class II periodontal furcation involvement.

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A feasibility study evaluating an in situ formed synthetic biodegradable membrane for guided bone regeneration in dogs

Cited by 50 publications

References 35 publications

Analysis of hydrolyzable polyethylene glycol hydrogels and deproteinized bone mineral as delivery systems for glycosylated and non-glycosylated bone morphogenetic protein-2

Analysis of hydrolyzable polyethylene glycol hydrogels and deproteinized bone mineral as delivery systems for glycosylated and non-glycosylated bone morphogenetic protein-2

Surface Properties and MC3T3-E1 Cell Response of Cortical Bone Allografts Modified with Low-Concentration Phosphoric Acid

A novel porcine collagen GTR membrane for treatment of Class II molar furcation involvement

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