A feasibility trial of genomics‐based diagnosis detecting insecticide resistance of the diamondback moth

Abstract

BACKGROUND Insecticide resistance management has been key for crop protection for over 70 years and is increasingly important because the development of new active ingredients has decreased in recent years. By monitoring the development of resistance in a timely manner, we can effectively prolong insecticide efficacy. Genomic‐based diagnosis can reliably predict resistance development if information on resistant mutations against major pesticides is available. Here, we developed a feasibility trial of genomics‐based diagnosis of insecticide resistance in diamondback moth (Plutella xylostella) populations in Nagano Prefecture, Japan. Amplicon sequencing analyses using a next‐generation sequencer (Illumina MiSeq) for major insecticides, including diamides, pyrethroids, Bacillus thuringiensis (Bt) toxin (Cry1Ac), organophosphates, and spinosyns, were conducted. RESULTS Mutations related to the resistance of pyrethroids, organophosphates, and diamides (flubendiamide and chlorantraniliprole) prevailed, while those of a diamide (cyantraniliprole), Bt (Cry1Ac), and spinosyns were scanty, suggesting that they are still effective. The results of the genomics‐based diagnosis were generally concordant with the results of bioassays. Resistance development tendencies were generally uniform across Nagano. CONCLUSION An insecticide‐resistance management campaign can be conducted in Nagano Prefecture with a quick genomic‐based diagnosis in early spring while bioassay is the only option for monitoring resistances whose mutations are unavailable. Our study is the first step in the future management of insecticide resistance in all significant pests. © 2022 Society of Chemical Industry.

A high‐throughput KASP assay provides insights into the evolution of multiple resistant mutations in populations of the two‐spotted spider mite Tetranychus urticae across China

“…The two mutations in the primer region led to the wrong determination of a heterozygote site to a homozygous site in one testing individual. The genomics‐based method might complement the KASP assay for high‐throughput genotyping of multiple mutations nearby 62 . Nevertheless, we provided a high‐throughput and accurate method to detect resistant mutations in the TSSM and other pests generally.…”

A comprehensive assessment of insecticide resistance mutations in source and immigrant populations of the diamondback moth Plutella xylostella (L.)

“…The KASP platform can perform 43 008 reactions (1536‐well plate × 28 channel) at one time by using the water bath PCR of a Hydrocycler‐16 machine (LGC Ltd). The resulting genotype can be immediately obtained and compared with next‐generation sequencing based methods, which need a relatively long time for sequencing and data analysis 37,69 . In our study, we used primers for a KASP assay of 14 resistant mutations of DBM.…”

“…be immediately obtained and compared with next-generation sequencing based methods, which need a relatively long time for sequencing and data analysis. 37,69 In our study, we used primers for a KASP assay of 14 resistant mutations of DBM. The established KASP assays provide an efficient approach for molecular diagnostics of DBM resistance that can be used for management.…”

Exploration of Synergistic Pesticidal Activities, Control Effects and Toxicology Study of a Monoterpene Essential Oil with Two Natural Alkaloids

“…In recent years, with the continuous expansion of vegetable cultivation area, the damage of T. urticae becomes more and more serious. The role of chemical insecticides in the management of these pests was indisputable; however, the extensive and irrational use of chemical agrochemicals has resulted in increasing resistances and environmental problems [6][7][8]. Cantharidin and (S)-(-)-palasonin showed different toxicity to P. xylostella, with median lethal concentrations, and quinolizidine alkaloids and neem-based products exhibited moderate acaricidal activities against T. urticae [9][10][11].…”

“…chemical agrochemicals has resulted in increasing resistances and environmental problems [6][7][8]. Cantharidin and (S)-(-)-palasonin showed different toxicity to P. xylostella, with median lethal concentrations, and quinolizidine alkaloids and neem-based products exhibited moderate acaricidal activities against T. urticae [9][10][11].…”