A feasible and practical 1 H NMR analytical method for the quality control and quantification of bioactive principles in Lycii Fructus

Abstract

Lycii Fructus, a solanaceous drug, is widely used as functional foods and in Traditional Chinese Medicine. Samples collected from different regions of China have been found to be not identical in chemical compositions which might affect the biological activities. Although many chromatographic and spectrometric methods have been reported to determine the concentration of betaine and other bioactive amino acids, disturbance resulted from other polar substances with low UV-absorbance and expensive mass facilities reduced the applicability of these techniques. In the present study, the strong cation exchange solid phase extraction procedure incorporated with H NMR was successfully developed as a rapid and reliable method that can simultaneously determine betaine, citric acid, threonine, alanine, and proline in various Lycii Fructus. In addition, ERETIC 2 method based on PULCON principle was also applied and compared with conventional method. This feasible and practical method offers a very powerful tool for the quality control of commercial Lycii Fructus from different sources.

Kunxian Capsule for Rheumatoid Arthritis: Inhibition of Inflammatory Network and Reducing Adverse Reactions Through Drug Matching

“…Up to now, 18 amino acids have been identified, including 8 essential amino acids. This is the only plant that has been reported to contain taurine (Luo et al, 2004;Tian and Wang, 2006;Hsieh et al, 2018). TNF, tumor necrosis factor; OCP, osteoclast precursors; DC, dendritic cell; LPS, lipopolysaccharide; NF-kB, nuclear factor kappa-B; FLS, fibroblast-like synoviocytes; SF, synovial fibroblasts; AIA, adjuvant-induced arthritis; AMP, adenosine monophosphate; mTOR, mammalian target of rapamycin; TGF, transforming growth factor; SMAD, suppressor of mothers against decapentaplegic; AKT, protein kinase B (PKB), also known as AKT; CCND2, cyclin D2.…”

Absolute Quantification of Isoflavones in the Flowers of Pueraria lobata by qHNMR

“…qHNMR is a powerful analytical tool for purity determination and quantification of compounds in mixtures. There is a growing interest in the application of qHNMR methods to quantify various classes of components in natural products [22,23]. qHNMR is preferable over conventional chromatography-based quantitative methods because it allows for the simultaneous identification and quantification of the complex matrix in a single run.…”

“…performance liquid chromatography-photodiode array (HPLC-PDA), liquid chromatog raphy-electrospray ionization-tandem mass spectrometry (LC-ESI-MS) [18], liquid chro matography coupled with triple stage quadrupole tandem mass spectrometry (TSQ MS/MS) [19], ultra-performance liquid chromatography-electrospray ionization-tandem mass spectroscopy (UPLC-ESI-MS/MS) [20], and quantitative 1 H NMR (qHNMR) [14,21 qHNMR is a powerful analytical tool for purity determination and quantification o compounds in mixtures. There is a growing interest in the application of qHNMR meth ods to quantify various classes of components in natural products [22,23]. qHNMR is pre erable over conventional chromatography-based quantitative methods because it allow for the simultaneous identification and quantification of the complex matrix in a singl run.…”

A Rapid and Feasible 1H-NMR Quantification Method of Ephedrine Alkaloids in Ephedra Herbal Preparations

“…Recently, 1 H-NMR spectroscopy was developed as an important tool for the quality control of phytochemical preparations [20][21][22][23][24][25][26][27][28], clinical diagnosis, and monitoring of treatment [29]. The advantages of the quantitative NMR (qNMR) method are manifold.…”