A feasible electrochemical biosensor for determination of glucose based on Prussian blue – Enzyme aggregates cascade catalytic system

Yan, Long; Miao, Kunpeng; Ma, Pengcheng; Ma, Xiaoyan; Bi, R.; Chen, Fang

doi:10.1016/j.bioelechem.2021.107838

Cited by 24 publications

(9 citation statements)

References 44 publications

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“…We have shown that bi-enzymatic cascades layer-by-layer constructed with GOx or GlOx, oxidising their substrates by O 2 with a formation of H 2 O 2 , and either peroxidase or peroxidase mimic -the hemin/PEI complex, electrocatalytically reducing H 2 O 2 at the electrode surface, could be successfully used to sense glucose and glutamate at moderate potentials and compete with the existing enzymatic-cascade-based electrode designs. [32][33][34][35][36][37][38][39][40][41][42] However, the cascade layer-by-layer assembly of enzymes on 5 μm carbon fibre microelectrodes resulted in accelerated mass-transfer of the enzymatically produced H 2 O 2 into solution and not to the sensing layer, disabling the ability of the enzymatic cascades to sense H 2 O 2 produced enzymatically from glucose or glutamate and O 2 . Considering the diffusing layer thickness for H 2 O 2 and the timescale of the experiments, we showed that as the electrode size/radius becomes less than 176 μm, the accelerated radial diffusion of H 2 O 2 from the electrode interferes with electrocatalytic reduction of H 2 O 2 .…”

Section: Discussionmentioning

confidence: 99%

Glucose and Glutamate Detection by Oxidase/Hemin Peroxidase Mimic Cascades Assembled on Macro‐ and Microelectrodes

Lopes,

Kaewjua,

Shipovskov

et al. 2024

ChemElectroChem

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Enzymatic cascades are routinely used for electroanalysis of redox inactive species that can be enzymatically converted into species electroactive at moderate potentials, such as H2O2 produced by FAD‐dependent oxidases oxidising their substrates by O2. However, such cascades adaptation to micro‐biosensors is limited by enhanced mass‐transfer of produced H2O2 into solution, not to the sensing layer. Here, bi‐enzyme sensors for glucose or glutamate, produced by cross‐linking peroxidase and oxidases on carbon‐nanotube‐modified graphite macro‐electrodes (Gr), showed the from 0.1 to 10 mM glucose/glutamate linear response, while bio‐modified 5 μm carbon fibre electrodes (CFE) were mute due to fast transfer of enzymatically produced H2O2 into solution. By replacing peroxidase with peroxidase‐mimicking hemin in polyethyleneimine, the sensitivity of detection at Gr improved 3‐fold, enabling 2.8 μM glucose and 4.5 μM glutamate limits of detection, but not at CFE. Fast mass‐transfer of H2O2 from CFE to solution was restricted by the Nafion membrane facilitating glucose detection at CFE with a sensitivity of 1.67 A cm−2 M−1, at −0.6 V, escaping interference from other brain‐fluid components, redox‐inactive at this potential. Such membrane‐restricted cascade system provides the analytical access to a large group of enzymes that can be integrated in multiple enzymatic cascades for biosensing at microelectrodes.

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Section: Discussionmentioning

confidence: 99%

Glucose and Glutamate Detection by Oxidase/Hemin Peroxidase Mimic Cascades Assembled on Macro‐ and Microelectrodes

Lopes,

Kaewjua,

Shipovskov

et al. 2024

ChemElectroChem

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“…For electrodeposition of PB layers, 0.1 M KCl + 0.01 M HCl supported electrolyte solution (solution 1) and 3.0 mM K 3 Fe(CN) 6 + 3.0 mM FeCl 3 mixture dissolved in solution 1 (solution 2) were prepared. The cleaned Au electrode was electrochemically deposited by applying a potential of 0.4 V for 100 s in the stir-free solution 2, and the PB layer was activated in solution 1 through CV, scanning range of −0.05 to +0.35 V until the presence of a steady CV profile [43]. Finally, the electrode was dried at 65 • C for 30 min to get a steady PB active layer.…”

Section: Electrode Treatmentmentioning

confidence: 99%

Study of electrochemical biosensor for determination of glucose based on Prussian blue/gold nanoparticles-chitosan nanocomposite film sensing interface

Peng,

Li,

Liao

et al. 2023

Meas. Sci. Technol.

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A highly electroactive bilayer composite film sensing interface of Prussian Blue (PB)/gold nanoparticles-chitosan (AuNPs-CS) was modified on Au electrode through electrochemical deposition and coating method followed by integrating glucose oxidase (GOx) into the interfacial matrix to fabricate a high-performance glucose biosensor. The excellent electrocatalytic ability of the PB/AuNPs-CS composite film sensing interface for H2O2 was evaluated, which has a broad linear response of 0.01-7.95 mM, with a low detection limit (LOD) 0.269 μM and a high sensitivity of 511.82 μA·mM-1·cm-2. The enhanced electrocatalytic activity of this sensing interface for H2O2 is attributed to the protection from the network CS film to PB and the synergistic effect of PB and AuNPs. Consequently, an electrochemical biosensing interface was constructed with GOx immobilized as a model enzyme. The PB/GOx-AuNPs-CS biosensing nanocomposite film was capable of a fast steady-state response time (within 2s) and high sensitivity to glucose with a wide linear range of: 0.025~2.00 mM (R2=0.99), with a sensitivity of 40.41 μA·mM-1·cm-2 and a LOD of 1.62 μM; and 2.00~6.50 mM (R2=0.98), with a sensitivity of 8.90 μA·mM-1·cm-2 and a LOD of 7.16 μM. The biosensor has good anti-interference and selectivity, which provides a promising wide linear range platform for clinical blood glucose detection in the future.

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“…Then, the PB/AuNS/GR electrode was dried at 50 • C for about 15 min. and characterized by an FE-SEM [16,24].…”

Section: The Preparation Modification and Characterization Of The Gra...mentioning

confidence: 99%

“…PB can be easily synthesized on the electrode surface and can replace the electron-transfer mediator present in the solution [14,15], thereby directly generating an electrochemical signal. This ensures the advancement of reagentless electrochemical glucose biosensors, where all necessary components are integrated, eliminating the need for reagent injection during the analysis process [16,17]. Unfortunately, PB-modified layers exhibit limited operational stability, particularly in neutral and alkaline conditions.…”

Section: Introductionmentioning

confidence: 99%

The Development of Reagentless Amperometric Glucose Biosensor Based on Gold Nanostructures, Prussian Blue and Glucose Oxidase

Sakalauskiene,

Brasiunas,

Popov

et al. 2023

Biosensors

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Precise blood glucose detection plays a crucial role in diagnosing and medicating diabetes, in addition to aiding diabetic patients in effectively managing their condition. In this research, a first-generation reagentless amperometric glucose biosensor was developed by combining the graphite rod (GR) electrode modification by gold nanostructures (AuNS) and Prussian blue (PB) with glucose oxidase (GOx)—an enzyme that can oxidize glucose and produce H2O2. Firstly, AuNS was electrochemically deposited on the GR electrode (AuNS/GR), and then PB was electrochemically synthesized on the AuNS/GR electrode (PB/AuNS/GR). Finally, GOx was immobilized over the PB/AuNS nanocomposite with the assistance of Nafion (Nf) (Nf-GOx/PB/AuNS/GR). An application of PB in the design of a glucose biosensor enables an easy electrochemical reduction and, thus, the determination of the H2O2 produced during the GOx-catalyzed oxidation of glucose in the sample at a low operation potential of −0.05 V vs. Ag/AgCl/KCl3 mol L−1. In addition, AuNS increased the electrochemically active surface area, improved the GOx immobilization and ensured a higher analytical signal. The developed glucose biosensor based on the Nf-GOx/PB/AuNS/GR electrode exhibited a wide linear range, from 0.025 to 1 mmol L−1 of glucose, with a 0.0088 mmol L−1 limit of detection, good repeatability and high selectivity over electroactive interfering substances. The developed biosensor is convenient for the determination of glucose in the physiological environment.

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A feasible electrochemical biosensor for determination of glucose based on Prussian blue – Enzyme aggregates cascade catalytic system

Cited by 24 publications

References 44 publications

Glucose and Glutamate Detection by Oxidase/Hemin Peroxidase Mimic Cascades Assembled on Macro‐ and Microelectrodes

Glucose and Glutamate Detection by Oxidase/Hemin Peroxidase Mimic Cascades Assembled on Macro‐ and Microelectrodes

Study of electrochemical biosensor for determination of glucose based on Prussian blue/gold nanoparticles-chitosan nanocomposite film sensing interface

The Development of Reagentless Amperometric Glucose Biosensor Based on Gold Nanostructures, Prussian Blue and Glucose Oxidase

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