A Ferritin Photochemical Synthesis of Monodispersed Silver Nanoparticles That Possess Antimicrobial Properties

Petrucci, Oscar D.; Hilton, Robert J.; Farrer, Jeffrey K.; Watt, Richard K.

doi:10.1155/2019/9535708

Cited by 17 publications

(8 citation statements)

References 56 publications

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“…ApoFTL exhibits a broad first protein elution with two overlapping peaks at 8.9 and 9.4 mL and a second elution peak at 10.8 mL (Figure 3A). These elution profiles indicate two different subpopulations of ferritin in the original sample that have also been reported by other authors [22,[45][46][47]. The first protein elution peak with a higher molecular weight corresponds to ferritin cage oligomers (dimers or trimers), while the second elution peak contains ferritin cage monomers, corroborated by native PAGE analysis (Figure S1).…”

Section: Gold Nanoparticles Synthesis and Characterizationsupporting

confidence: 86%

Enhanced Cellular Uptake of H-Chain Human Ferritin Containing Gold Nanoparticles

et al. 2021

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Gold nanoparticles (AuNP) capped with biocompatible layers have functional optical, chemical, and biological properties as theranostic agents in biomedicine. The ferritin protein containing in situ synthesized AuNPs has been successfully used as an effective and completely biocompatible nanocarrier for AuNPs in human cell lines and animal experiments in vivo. Ferritin can be uptaken by different cell types through receptor-mediated endocytosis. Despite these advantages, few efforts have been made to evaluate the toxicity and cellular internalization of AuNP-containing ferritin nanocages. In this work, we study the potential of human heavy-chain (H) and light-chain (L) ferritin homopolymers as nanoreactors to synthesize AuNPs and their cytotoxicity and cellular uptake in different cell lines. The results show very low toxicity of ferritin-encapsulated AuNPs on different human cell lines and demonstrate that efficient cellular ferritin uptake depends on the specific H or L protein chains forming the ferritin protein cage and the presence or absence of metallic cargo. Cargo-devoid apoferritin is poorly internalized in all cell lines, and the highest ferritin uptake was achieved with AuNP-loaded H-ferritin homopolymers in transferrin-receptor-rich cell lines, showing more than seven times more uptake than apoferritin.

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Section: Gold Nanoparticles Synthesis and Characterizationsupporting

confidence: 86%

Enhanced Cellular Uptake of H-Chain Human Ferritin Containing Gold Nanoparticles

et al. 2021

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“…AgNPs differ from bulk and micron size silver in their size, shape, and stability [ 4 ]. Several techniques are commonly employed to synthesize AgNPs, including chemical synthesis [ 5 ], electrochemical synthesis [ 6 ], radiation synthesis [ 7 ] and photochemical synthesis [ 8 ]. In comparison with other methods, biological synthesis of AgNPs has received a great interest due to its eco-friendly mode of synthesis with passable biomedical properties [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

A Bottom-Up Synthesis Approach to Silver Nanoparticles Induces Anti-Proliferative and Apoptotic Activities Against MCF-7, MCF-7/TAMR-1 and MCF-10A Human Breast Cell Lines

et al. 2020

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A bottom-up approach for synthesizing silver nanoparticles (AgNPs-GA) phytomediated by Garcinia atroviridis leaf extract is described. Under optimized conditions, the AgNPs-GA were synthesized at a concentration of 0.1 M silver salt and 10% (w/v) leaf extract, 1:4 mixing ratio of reactants, pH 3, temperature 32 °C and 72 h reaction time. The AgNPs-GA were characterized by various analytical techniques and their size was determined to be 5–30 nm. FTIR spectroscopy indicates the role of phenolic functional groups in the reduction of silver ions into AgNPs-GA and in supporting their subsequent stability. The UV-Visible spectrum showed an absorption peak at 450 nm which reflects the surface plasmon resonance (SPR) of AgNPs-GA and further supports the stability of these biosynthesized nanoparticles. SEM, TEM and XRD diffractogram analyses indicate that AgNPs-GA were spherical and face-centered-cubic in shape. This study also describes the efficacy of biosynthesized AgNPs-GA as anti-proliferative agent against human breast cancer cell lines, MCF-7 and MCF-7/TAMR-1. Our findings indicate that AgNPs-GA possess significant anti-proliferative effects against both the MCF-7 and MCF-7/TAMR-1 cell lines, with inhibitory concentration at 50% (IC50 values) of 2.0 and 34.0 µg/mL, respectively, after 72 h of treatment. An induction of apoptosis was evidenced by flow cytometry using Annexin V-FITC and propidium iodide staining. Therefore, AgNPs-GA exhibited its anti-proliferative activity via apoptosis on MCF-7 and MCF-7/TAMR-1 breast cancer cells in vitro. Taken together, the leaf extract from Garcinia atroviridis was found to be highly capable of producing AgNPs-GA with favourable physicochemical and biological properties.

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“…Based on the data of particle size analyzer which showed in figure 6. Silver nanoparticles have a particle size distribution of 43.5 nm and a polydispersity index (PDI) value of 0.4 [45].…”

Section: Resultsmentioning

confidence: 99%

“…Meanwhile, there are many reducing agents which used to reduce Ag + to Ag 0 ions on silver nitrate and silver chloride precursors such as NaOH [16], ammonia [17], sodium citrate and NaCl [18], and polyvinyl alcohol [19]. However, it is hazardous and not environmentally friendly.…”

Section: Introductionmentioning

confidence: 99%

Bioreduction and Characterization of Silver Nanoparticles From Oil Palm Empty Fruit Bunch

Suhartati

Saputra²,

Rahmi

et al. 2020

jsmi

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BIOREDUCTION AND CHARACTERIZATION OF SILVER NANOPARTICLES FROM OIL PALM EMPTY FRUIT BUNCH (OPEFB). The synthesis of silver nanoparticles was successfully carried out by extracting oil palm empty fruit bunch. The precursor used was silver nitrate (AgNO3) with a concentration of 9x10-4 M and 5 wt% of the oil palm empty fruit bunch extract. OPEFB acted as a capping agent in the synthesis of silver nanoparticles. The bioreduction method Ag+ to Ag0 produced a silver nanoparticle colloid in brown color. The results of the UV-Vis spectrophotometer showed the silver nanoparticles colloids spectrum at a wavelength of 420 nm with an absorbance value of 0.5. FTIR shows the reduction and shift of absorption peak in the hydroxyl functional group (-OH) at wavenumbers of 3323 cm-1 and the presence of absorption peaks at 560 cm-1. While, XRD pattern showed the specific crystallinity peaks of silver nanoparticles at 2θ: 33.24°; 39.98°; 61.23°; dan 79.13° respectively with the face-centered cubic crystal structure (FCC) and crystallite size of 15 nm. PSA analysis showed two specific peaks with an average size distribution silver nanoparticles of 43.5 nm and a PDI value of 0.4. Analysis of TEM shows the average particle size of 20 nm with a spherical particle shape.

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A Ferritin Photochemical Synthesis of Monodispersed Silver Nanoparticles That Possess Antimicrobial Properties

Cited by 17 publications

References 56 publications

Enhanced Cellular Uptake of H-Chain Human Ferritin Containing Gold Nanoparticles

Enhanced Cellular Uptake of H-Chain Human Ferritin Containing Gold Nanoparticles

A Bottom-Up Synthesis Approach to Silver Nanoparticles Induces Anti-Proliferative and Apoptotic Activities Against MCF-7, MCF-7/TAMR-1 and MCF-10A Human Breast Cell Lines

Bioreduction and Characterization of Silver Nanoparticles From Oil Palm Empty Fruit Bunch

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