A Fibrin Coating Method of Polypropylene Meshes Enables the Adhesion of Menstrual Blood-Derived Mesenchymal Stromal Cells: A New Delivery Strategy for Stem Cell-Based Therapies

Abstract

Polypropylene (PP) mesh is well-known as a gold standard of all prosthetic materials of choice for the reinforcement of soft tissues in case of hernia, organ prolapse, and urinary incontinence. The adverse effects that follow surgical mesh implantation remain an unmet medical challenge. Herein, it is outlined a new approach to allow viability and adhesion of human menstrual blood-derived mesenchymal stromal cells (MenSCs) on PP surgical meshes. A multilayered fibrin coating, based on fibrinogen and thrombin from a commercial fibrin sealant, was optimized to guarantee a homogeneous and stratified film on PP mesh. MenSCs were seeded on the optimized fibrin-coated meshes and their adhesion, viability, phenotype, gene expression, and immunomodulatory capacity were fully evaluated. This coating guaranteed MenSC viability, adhesion and did not trigger any change in their stemness and inflammatory profile. Additionally, MenSCs seeded on fibrin-coated meshes significantly decreased CD4+ and CD8+ T cell proliferation, compared to in vitro stimulated lymphocytes (p < 0.0001). Hence, the proposed fibrin coating for PP surgical meshes may allow the local administration of stromal cells and the reduction of the exacerbated inflammatory response following mesh implantation surgery. Reproducible and easy to adapt to other cell types, this method undoubtedly requires a multidisciplinary and translational approach to be improved for future clinical uses.

Cytotoxicity Assessment of a New Design for a Biodegradable Ureteral Mitomycin Drug-Eluting Stent in Urothelial Carcinoma Cell Culture

“…Mitomycin C cytotoxicity was assessed by a viability assay using CCK-8 (Cell Counting Kit-8, Boster Biological Technology, Pleasanton, CA, USA). The protocol was carried out according to the manufacturer’s recommendations, and the absorbance at 450 nm was recorded using a Synergy™ Mx microplate reader (BioTek Instruments, Winooski-Vermont-USA) [ 21 ]. Briefly, after the first 24 h of incubation, the cell viability assay was performed with CCK-8 at T0 in two wells from each experimental group.…”

“…Finally, absorbance was measured using a Synergy™ Mx microplate reader (BioTek Instruments, Winooski-Vermont-USA) at 450 nm. After ending the CCK-8 assay at T0, the study groups were maintained in culture on their respective plates for 3 h and 6 h at 37 °C in the HeraCell™ 150i CO 2 incubator (Thermo Scientific™, Waltham, MA, USA), with six replicates per experimental group [ 21 ]. The absorbance of formazan was measured at 450 nm using the plate reader mentioned above.…”

The first results of obtaining a hybrid hydrogel based on fibrin and polyvinyl alcohol: comparison with monocomponent hydrogels

“…Fibrin is a natural polymer with unique biological properties useful for tissue engineering. It is an ideal cell carrier with a high ability to retain cells on the surface [1][2][3], to bind and dosed release of growth factors for supports angiogenesis and tissue repair [4,5]. Autologous fi brin can be obtained simply and in suffi cient quantities from the patient's own blood, making it a very attractive material for personalized tissue engineering [6].…”

“…Фибрин представляет собой природный полимер с уникальными для тканевой инженерии биологическими свойствами. Он является не только идеальным клеточным носителем с высокой способностью к удержанию клеток на поверхности [1][2][3], но, и благодаря способности связывать и дозированно высвобождать ростовые факторы, активно поддерживает ангиогенез и репарацию тканей [4,5]. В отличие от других биополимеров (коллагены различных типов, фибронектин, эластин), аутологичный фибрин можно просто и в достаточном количестве получать из собственной крови пациента, что делает его весьма привлекательным материалом для персонифицированной тканевой инженерии [6].…”

Dysfunction of metabolic activity of bone marrow mesenchymal stem cells in aged mice

“… 1 , 2 , 3 , 4 However, previous studies have shown that the functional properties of MSCs would be impaired due to the aging or disease status of donors, which may significantly affect the eventual efficiency. 5 , 6 Aging is a complex, gradual, and inevitable physiological process, accompanied by the accumulation of damage macromolecules, leading to organ dysfunction and the senescence of MSCs, with the character of significantly impaired biological properties of MSCs. 7 , 8 , 9 Several methods have been tried to modify the senescence of MSCs, but the effect remains unsatisfied.…”