A fibrin-supported myocardial organ culture for isolation of cardiac stem cells via the recapitulation of cardiac homeostasis

Kim, Jong-Tae; Chung, Hye Jin; Seo, Ji Yeon; Yang, Young Il; Choi, Moon Young; Kim, Hyeong In; Yang, Tae Hyun; Lee, Won Jin; Youn, Young Chul; Kim, Hye Jung; Kim, Yeon Mee; Lee, Hyukjin; Jang, Yang Soo; Lee, Seung Jin

doi:10.1016/j.biomaterials.2015.01.041

Cited by 12 publications

(9 citation statements)

References 65 publications

(144 reference statements)

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“…We used the CMSCs obtained by ex vivo expansion of human heart tissue using 3D culture as previously described (25). These CMSCs did not form cardiospheres, but shared similar phenotypes with CDCs of CD90 The CMSCs were distinguished from MSCs in the expression of CD90 (22).…”

Section: Discussionmentioning

confidence: 99%

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Characterization of Human Cardiac Mesenchymal Stromal Cells and Their Extracellular Vesicles Comparing With Human Bone Marrow Derived Mesenchymal Stem Cells

et al. 2020

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Cardiac regeneration with adult stem-cell (ASC) therapy is a promising field to address advanced cardiovascular diseases. In addition, extracellular vesicles (EVs) from ASCs have been implicated in acting as paracrine factors to improve cardiac functions in ASC therapy. In our work, we isolated human cardiac mesenchymal stromal cells (h-CMSCs) by means of three-dimensional organ culture (3D culture) during ex vivo expansion of cardiac tissue, to compare the functional efficacy with human bone-marrow derived mesenchymal stem cells (h-BM-MSCs), one of the actively studied ASCs. We characterized the h-CMSCs as CD90 low , c-kit negative , CD105 positive phenotype and these cells express NANOG, SOX2, and GATA4. To identify the more effective type of EVs for angiogenesis among the different sources of ASCs, we isolated EVs which were derived from CMSCs with either normoxic or hypoxic condition and BM-MSCs. Our in vitro tube-formation results demonstrated that the angiogenic effects of EVs from hypoxia-treated CMSCs (CMSC-Hpx EVs) were greater than the well-known effects of EVs from BM-MSCs (BM-MSC EVs), and these were even comparable to human vascular endothelial growth factor (hVEGF), a potent angiogenic factor. Therefore, we present here that CD90 low c-kit negative CD105 positive CMSCs under hypoxic conditions secrete functionally superior EVs for in vitro angiogenesis. Our findings will allow more insights on understanding myocardial repair. [BMB Reports 2020; 53(2): 118-123]

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Section: Discussionmentioning

confidence: 99%

“…CMSCs were explanted from cadaveric tissue and expanded as described previously (25). Briefly, after 14 d of dynamic tissue culture, outgrown cells were collected and suspended in growth-culture media for a conventional monolayer culture condition.…”

Section: Human Cmscs Preparationmentioning

confidence: 99%

Characterization of Human Cardiac Mesenchymal Stromal Cells and Their Extracellular Vesicles Comparing With Human Bone Marrow Derived Mesenchymal Stem Cells

et al. 2020

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“…This study demonstrated a selective isolation of CSCs from the fibrin by matrix restricted fibrinolysis without any sophisticated tissue dissociation and purification steps. 48 Platelet fibrin gel (also known as a platelet fibrin scaffold) and stem cells have been investigated for therapeutic cardiac repair. For example, Shen et al studied the effects of matrix metalloproteinases (MMPs) on the performance of a platelet fibrin gel spiked with CSCs for heart repair.…”

Section: Fibrinmentioning

confidence: 99%

<p>Biodegradable Nanopolymers in Cardiac Tissue Engineering: From Concept Towards Nanomedicine</p>

Nasr¹,

Rabiee²,

Hajebi³

et al. 2020

IJN

101

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Cardiovascular diseases are the number one cause of heart failure and death in the world, and the transplantation of the heart is an effective and viable choice for treatment despite presenting many disadvantages (most notably, transplant heart availability). To overcome this problem, cardiac tissue engineering is considered a promising approach by using implantable artificial blood vessels, injectable gels, and cardiac patches (to name a few) made from biodegradable polymers. Biodegradable polymers are classified into two main categories: natural and synthetic polymers. Natural biodegradable polymers have some distinct advantages such as biodegradability, abundant availability, and renewability but have some significant drawbacks such as rapid degradation, insufficient electrical conductivity, immunological reaction, and poor mechanical properties for cardiac tissue engineering. Synthetic biodegradable polymers have some advantages such as strong mechanical properties, controlled structure, great processing flexibility, and usually no immunological concerns; however, they have some drawbacks such as a lack of cell attachment and possible low biocompatibility. Some applications have combined the best of both and exciting new natural/ synthetic composites have been utilized. Recently, the use of nanostructured polymers and polymer nanocomposites has revolutionized the field of cardiac tissue engineering due to their enhanced mechanical, electrical, and surface properties promoting tissue growth. In this review, recent research on the use of biodegradable natural/synthetic nanocomposite polymers in cardiac tissue engineering is presented with forward looking thoughts provided for what is needed for the field to mature.

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“…After 7 d of culture, outgrown CSCs were recovered by the selective degradation of the fibrin using 5000 units of urokinase (Sigma-Aldrich) for 30 min in the presence of 30% calf serum (CS) in DMEM. When the fibrin was degraded, the released CSCs from the fibrin were collected and centrifuged at 150 g for 5 min, as described in our previous work [30]. These collected CSCs were suspended in the growth culture media, plated into a 150-mm tissue culture dish, and then cultured under conventional monolayer culture conditions.…”

Section: Isolation and Culture Of Cscsmentioning

confidence: 99%

Intracellular transduction of TAT-Hsp27 fusion protein enhancing cell survival and regeneration capacity of cardiac stem cells in acute myocardial infarction

A fibrin-supported myocardial organ culture for isolation of cardiac stem cells via the recapitulation of cardiac homeostasis

Cited by 12 publications

References 65 publications

Characterization of Human Cardiac Mesenchymal Stromal Cells and Their Extracellular Vesicles Comparing With Human Bone Marrow Derived Mesenchymal Stem Cells

Characterization of Human Cardiac Mesenchymal Stromal Cells and Their Extracellular Vesicles Comparing With Human Bone Marrow Derived Mesenchymal Stem Cells

<p>Biodegradable Nanopolymers in Cardiac Tissue Engineering: From Concept Towards Nanomedicine</p>

Intracellular transduction of TAT-Hsp27 fusion protein enhancing cell survival and regeneration capacity of cardiac stem cells in acute myocardial infarction

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