Palaeointensity data from the Precambrian are key to understanding the timing of the Earth’s Inner Core Nucleation (ICN). Due to the scarcity of data, the ICN timing is still poorly constrained and is thought to have occurred between 2500 to 500 Ma. Numerical dynamo simulation models predict an increase in entropy, a stronger driving force for convection that could affect the field strength and show an anomaly in the palaeointensity record at ICN. We present new estimates of the geomagnetic field intensity (palaeointensity) from the Mid-Mesoproterozoic (1406 ± 1424 Ma) Nova Guarita dyke swarm, in the northern Mato Grosso State (SW Amazon Craton, Brazil). To obtain palaeointensity estimates, we used the non-heating Preisach method, with palaeointensity criteria at the specimen, and site level. Five sites provided accepted palaeointensity results, yielding virtual dipole moment (VDM) estimate of 65 ± 12 ZAm2 at 1416 ± 13 Ma, 53 ± 4 ZAm2 at 1418 ± 3 Ma, 12 ± 2 and 8 ± 2 ZAm2 at 1418 ± 5 Ma, and 71 ± 16 ZAm2 at 1424 ± 16 Ma, thus an average estimate of 43 ± 30 ZAm2 for ∼1410 Ma. The estimate is similar to the average VDM data (∼50 ZAm2), calculated for the period from 1600 to 1000 Ma. This average represents only a snapshot of the Earth’s magnetic field strength. While the new data are too limited in time to contribute directly to the question of ICN, they nevertheless contribute to constraints useful for assessing numerical simulations of the Mesoproterozoic geodynamo.