A Field Survey for Serogroups and the Satellite RNA of Cucumber Mosaic Virus

Kearney, Christopher Michel

doi:10.1094/phyto-80-1238

Cited by 18 publications

(8 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The largest proportion of isolates belonging to subgroup II was obtained from pepper -four of nine (44%). A similar, very high number of strains from subgroup II in isolates from pepper was recorded in New York, USA by Kearney et al (1990). Among 13 isolates from tobacco only one belonged to subgroup II (7.7%).…”

Section: Elisasupporting

confidence: 63%

Differentiation of Bulgarian Isolates from Cucumber Mosaic Virus by Serological Methods

Hristova¹,

Yordanova²,

Mavrodieva³

2002

Journal of Phytopathology

View full text Add to dashboard Cite

Cucumber mosaic virus (CMV) is of great importance to the Bulgarian economy and hence a detailed knowledge of its diversity under local geographic and climatic conditions is required. An extended study was carried out on CMV strains the currently occur in Bulgaria. Fifty‐one isolates and strains found in different regions and various crops were biologically characterized and serologically differentiated into subgroups I and II using different variants of enzyme‐linked immunosorbent assay (ELISA) [double antibody sandwich (DAS)‐, antigen‐coated plate (ACP)‐, triple antibody sandwich (TAS)‐ with poly and monoclonal antibodies] and immunodiffusion tests. The ELISA modifications with monoclonal antibodies individually (ACP) or in combination with polyclonal antibodies (TAS‐ELISA) are suitable for mass screening of CMV isolates. The hyperimmune sera against strains from CMV subgroups I and II were very efficient for use in isolate differentiation via gel double immunodiffusion. The results obtained correlated with the polymerase chain reaction and restriction fragment length polymorphism data reported by other authors. The majority of the isolates belonged to subgroup I, whereas 10, mainly from tomato and pepper, belonged to subgroup II. Most of the subgroup II isolates came from the north of Bulgaria. The results of the present study will help to clarify the virus epidemiology and to develop specific control measures.

show abstract

Section: Elisasupporting

confidence: 63%

Differentiation of Bulgarian Isolates from Cucumber Mosaic Virus by Serological Methods

Hristova¹,

Yordanova²,

Mavrodieva³

2002

Journal of Phytopathology

View full text Add to dashboard Cite

show abstract

“…Although CMV satellite RNAs have been reported in many different CMV isolates from all over the world, the frequency of satellite RNAs in field populations ofCMV seems to be usually low (KEARNEY et al 1990). Work on the evolution of satellite RNA populations under natural, field conditions derives mainly from the analysis of populations representing epidemics of tomato necrosis caused by 4S F. Garcia-Arenal and P. Palukaitis CMV + satellite RNA that occurred in southeastern Spain and southern Italy in the late 1980s.…”

Section: Genetic Variability Under Experimental and Natural Field Conmentioning

confidence: 99%

Structure and Functional Relationships of Satellite RNAs of Cucumber Mosaic Virus

García‐Arenal

Palukaitis²

1999

Current Topics in Microbiology and Immunology

View full text Add to dashboard Cite

“…Outbreaks of diseases incited by CMV have caused large losses in tomato and pepper crops in several Mediterranean (Gallitelli et al, 1988;Jorda et al, 1992;Marrou and Duteil, 1974) and Asian countries (Sayama et al, 1993;Tien and Wu, 1991). In the United States, high incidences of CMV have been reported in several states (Daniels and Campbell, 1992;Davis and Shifriss, 1983;Gonsalves et al, 1982;Kearney et al, 1990).…”

mentioning

confidence: 99%

Transgenic Tomato Plants Expressing Satellite RNA Are Tolerant to Some Strains of Cucumber Mosaic Virus

McGarvey¹,

Montasser²,

Kaper³

1994

jashs

View full text Add to dashboard Cite

Transgenic tomato plants (Lycopersicon esculentum Mill.) expressing cucumber mosaic virus (CMV) satellite RNA fused to a gene for β-glucuronidase were produced using Agrobacterium-mediated transformation. The R1 progeny of self-crossed R0 plants were challenge-inoculated with virion or RNA preparations of CMV or tomato aspermy virus (TAV). The transgenic plants challenged with CMV-1 showed mild disease symptoms in the first 2 weeks postchallenge followed by a decrease in symptoms, resulting in little difference between the transgenic and uninfected control group by the fourth week. Enzyme-linked immunosorbent assay results showed about a 10-fold decrease in virus accumulation in the transgenic plants compared to controls. Tolerance was evident only in plants that contained the recombinant insert and produced mature unit-length satellite RNA after CMV infection. Plants challenged with TAV showed no significant tolerance to virus-induced symptoms.

show abstract

A Field Survey for Serogroups and the Satellite RNA of Cucumber Mosaic Virus

Cited by 18 publications

References 0 publications

Differentiation of Bulgarian Isolates from Cucumber Mosaic Virus by Serological Methods

Differentiation of Bulgarian Isolates from Cucumber Mosaic Virus by Serological Methods

Structure and Functional Relationships of Satellite RNAs of Cucumber Mosaic Virus

Transgenic Tomato Plants Expressing Satellite RNA Are Tolerant to Some Strains of Cucumber Mosaic Virus

Contact Info

Product

Resources

About