A filter array based on the variable period grating array and Fabry-Perot resonator

Shi, Z.B.; Fang, Liang; Zhou, Chenyang

doi:10.1007/s11801-014-3159-y

Cited by 5 publications

(4 citation statements)

References 12 publications

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“…In this sense, RBC deformability can be a new biomarker and the fast and easy measurement methods are required. Former studies on RBC deformability have introduced some methodologies such as micropipette aspiration and optical tweezers where basically RBCs are stretched and the pressure or force for their extension is measured [15,16]. Whilst these studies have revealed useful RBCs mechanical properties, these methods reqire large amount of preparation and are very time consuming.…”

Section: Introductionmentioning

confidence: 99%

A microfluidic device for partial cell separation and deformability assessment

2013

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Blood flow in microcirculation shows several interesting phenomena that can be used to develop microfluidic devices for blood separation and analysis in continuous flow. In this study we present a novel continuous microfluidic device for partial extraction of red blood cells (RBCs) and subsequent measurement of RBC deformability. For this purpose, we use polydimethylsiloxane (PDMS) microchannels having different constrictions (25%, 50% and 75%) to investigate their effect on the cell-free layer (CFL) thickness and separation efficiency. By using a combination of image analysis techniques we are able to automatically measure the CFL width before and after an artificial constriction. The results suggest that the CFL width increases with enhancement of the constriction and contributes to partial cell separation. The subsequent measurements of RBCs deformation index reveal that the degree of deformation depends on the constriction geometries and hematocrit after the cell separation module. The proposed microfluidic device can be easily transformed into a simple, inexpensive and convenient clinical tool able to perform both RBC separation and deformability analysis in one single device. This would eliminate the need for external sample handling and thus reducing associated labor costs and potential human errors.

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Section: Introductionmentioning

confidence: 99%

A microfluidic device for partial cell separation and deformability assessment

2013

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“…16,17 These and other techniques were thoroughly reviewed by Kim et al 18 and are useful in measuring the RBC deformation under shear flow or the rheological properties of the RBC membrane, but are generally labor intensive, time consuming, and involve a typically difficult process of manipulation. Among these methods, the micropipette aspiration is the most popular single-cell technique to measure the mechanical properties of the RBC membrane.…”

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confidence: 99%

“…Among these methods, the micropipette aspiration is the most popular single-cell technique to measure the mechanical properties of the RBC membrane. 17 Briefly, the RBC membrane is aspirated by a negative pressure through a glass micropipette, having inner diameter much smaller than the RBC average size, forcing the micropipetted RBCs to adopt an extended "tongue" shape where its length is measured under a microscope. A major drawback of this technique is that direct contact between the cell surface and the wall cannot be avoided and the extensional rate is not constant in space and time.…”

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confidence: 99%

Human red blood cell behavior under homogeneous extensional flow in a hyperbolic-shaped microchannel

et al. 2013

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It is well known that certain pathological conditions result in a decrease of red blood cells (RBCs) deformability and subsequently can significantly alter the blood flow in microcirculation, which may block capillaries and cause ischemia in the tissues. Microfluidic systems able to obtain reliable quantitative measurements of RBC deformability hold the key to understand and diagnose RBC related diseases. In this work, a microfluidic system composed of a microchannel with a hyperbolic-shaped contraction followed by a sudden expansion is presented. We provide a detailed quantitative description of the degree of deformation of human RBCs under a controlled homogeneous extensional flow field. We measured the deformation index (DI) as well as the velocity of the RBCs travelling along the centerline of the channel for four different flow rates and analyze the impact of the particle Reynolds number. The results show that human RBC deformation tends to reach a plateau value in the region of constant extensional rate, the value of which depends on the extension rate. Additionally, we observe that the presence of a sudden expansion downstream of the hyperbolic contraction modifies the spatial distribution of cells and substantially increases the cell free layer (CFL) downstream of the expansion plane similarly to what is seen in other expansion flows. Beyond a certain value of flow rate, there is only a weak effect of inlet flow rates on the enhancement of the downstream CFL. These in vitro experiments show the potential of using microfluidic systems with hyperbolic-shaped microchannels both for the separation of the RBCs from plasma and to assess changes in RBC deformability in physiological and pathological situations for clinical purposes. However, the selection of the geometry and the identification of the most suitable region to evaluate the changes on the RBC deformability under extensional flows are crucial if microfluidics is to be used as an in vitro clinical methodology to detect circulatory diseases. V C 2013 AIP Publishing LLC. [http://dx

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“…Bu durum eritrositlerin şeklini tersinir olarak değiştirebilme yani deformabilite yeteneği ile mümkündür (1)(2)(3). Eritrosit deformabilitesi eritrositin geometrik şekli, sitoplazmik viskozitesi ve eritrosit membranının mekanik özellikleri tarafından belirlenmektedir (1,(4)(5)(6).…”

Section: Gi̇ri̇ş Ve Amaçunclassified

Effect of Endogenous and Exogenous Adenosine-Three Phosphate on Erythrocyte Deformability

Ülker¹

2017

Akd Med J

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ÖZAmaç: Eritrosit deformabilitesi eritrositlerin dolaşım sisteminde karşılaştıkları kuvvetlerin etkisi altın-da şekil değiştirebilme yeteneğidir. Bu parametre deneysel ve klinik hemoreoloji çalışmalarında sıklık-la kullanılarak eritrositlerin mekanik özellikleri değerlendirilmektedir. Eritrositler plazma içinde endojen ve eksojen adenozin tri-fosfat (ATP)ʼye sürekli maruz kalmaktadır. Eksojen ATPʼnin eritrositlerde hücre içi iyon dengesinde ve hücre hacminde değişmeye neden olduğu daha önce gösterilmiştir. Oysa hücre geometrisinden büyük oranda etkilenen eritrosit deformabilitesinin ATPʼden etkilenip etkilenmediği bilinmemektedir. Çalışmanın amacı eritrosit deformabilitesinin endojen ve eksojen ATPʼye yanıtlarının incelenmesidir. Gereç ve Yöntemler:Çalışmada gönüllü erkeklerden alınan venöz kan örnekleri kullanılmıştır. Kan örneklerinden eritrosit izolasyonu yapıldıktan sonra otolog plazma içinde hematokrit %40 olarak ayarlanmıştır. Eksojen ATP çalışmalarında purinerjik reseptör inhibitörü varlığında ve yokluğunda eritrosit süspansiyonlarına 100, 300 ve 500 µM ATP eklenmiş ve yarım saat inkübasyonun ardından deformabilite ölçümleri yapılmıştır. Endojen ATP çalışmalarında ATP kanal inhibitörü varlığında ve yokluğunda eritrositlere mekanik stres uygulanmış ve mekanik stresin hemen ardından eritrosit deformabilitesi ölçülmüştür.Bulgular: 300 ve 500 µM ATP eritrosit deformabilitesinde düşmeye neden olurken bu düşüş purinerjik reseptör inhibitörü varlığında ortadan kalkmıştır. Öte yandan mekanik stres eritrositlerden ATP salınımını uyarmış ve bu salınım ATP kanal inhibitörü ile ortadan kalkmıştır. Bu koşullar altında endojen ATP eritrosit deformabilitesinde bir değişikliğe neden olmamıştır. Sonuç:Çalışmanın sonuçları eksojen ATP'nin hücre membranında bulunan purinerjik reseptörler aracılığı ile eritrosit deformabilitesinde azalmaya neden olduğunu ilk defa göstermiştir. Öte yandan dolaşım sisteminde gözlenen mekanik stres düzeylerinde eritrositlerden salınan endojen ATPʼnin eritrosit deformabilitesini etkilemediği gösterilmiştir.Anahtar Sözcükler: Eritrosit, Deformabilite, ATP, Mekanik stres ABSTRACT Objective: Erythrocytes respond to forces by extensive changes in their shape, with the degree of deformation under a given force known as erythrocyte deformability. Erythrocytes are consistently exposed to endogenous and exogenous ATP in the plasma. Studies have shown that exogenous adenosine-three phosphate (ATP) causes the intracellular ionic balance to change and reduces the cell volume. Although it is well known that cell volume is one of the regulators of erythrocyte deformability, there is no study on the effect of ATP on erythrocyte deformability. The aim of this study was to investigate the effects of endogenous and exogenous ATP on erythrocyte deformability. Material and Methods:Erythrocytes isolated from volunteers were used in this study. After erythrocyte isolation, the hematocrit of erythrocyte suspensions was set to 40%. The effect of 100, 300 and 500 µM ATP on erythrocyte deformability was evaluated in the...

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A filter array based on the variable period grating array and Fabry-Perot resonator

Cited by 5 publications

References 12 publications

A microfluidic device for partial cell separation and deformability assessment

A microfluidic device for partial cell separation and deformability assessment

Human red blood cell behavior under homogeneous extensional flow in a hyperbolic-shaped microchannel

Effect of Endogenous and Exogenous Adenosine-Three Phosphate on Erythrocyte Deformability

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