Marine mollusk shells continuously incorporate oxygen isotope signatures
during growth that are representative of their surrounding environment
and thus produce valuable records of seawater temperature and oxygen
isotopic composition. These records of past environmental conditions can
be measured in situ at length-scales down to sub-daily growth increments
using high resolution ion microprobes (SIMS). However, the determination
of oxygen isotope ratios in aragonite, the most common shell mineral, is
hampered by a lack of ideal reference materials, limiting the accuracy
of isotope calibrations and temperature reconstructions. Here, we
cultured marine Anadara trapezia bivalves under controlled environmental
conditions at four seawater temperatures ranging from 13 to 28 °C. The
start of the growth period was marked in the crossed-lamellar shell
ultrastructure using strontium labelling, enabling precise targeting of
the SIMS analyses. A novel calibration method, specifically tailored to
analyses of biogenic aragonites with organic-inorganic architectures,
has been developed to correct matrix biases that affect the accuracy of
all such SIMS oxygen isotope analyses. The matrix fractionation bias
calibration was achieved by combining two aragonite reference materials
in a linear relationship, pinning the SIMS biases to the true
composition as a function of calcium abundances. The oxygen isotope
calibration provided a novel seawater temperature versus
seawater-corrected oxygen isotope fractionation relationship of T (°C) =
23.31 ± 0.34 - 4.31 · (δ18Oaragonite [‰ VPDB] - δ18Oseawater [‰
VSMOW] ± 0.22) that improves the applicability of in-situ oxygen
isotope-based paleo-environmental reconstructions of marine
bio-aragonite proxy archives.