A Fish Eye View: Retinal Morphogenesis from Optic Cup to Neuronal Lamination

Norden, Caren

doi:10.1146/annurev-cellbio-012023-013036

Cited by 4 publications

(1 citation statement)

References 109 publications

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“…The functions of the Hh, Wnt, Notch, and FGF signaling pathways have thoroughly investigated previously. Nonetheless, it is not uncommon to come across studies suggesting a specific role for for one signal while others advocate for the complete opposite [63,64]. Here, we outline our observations following chemical inhibition of Hh, Wnt, Notch, and FGF signaling pathways concerning the balance between proliferation and di↵erentiation, and their alignment with previous studies:…”

Section: Discussionmentioning

confidence: 54%

High-throughput three-dimensional characterization of morphogenetic signals during the formation of the vertebrate retina

Ledesma-Terrón,

Pérez-Dones,

Mazó-Durán

et al. 2024

Preprint

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The precise differentiation of neural progenitors during development ensures the correct cognitive, sensory and motor functions of higher organisms. This balance between self-renewal and terminal differentiation is regulated by a complex interplay of signaling pathways, that set the spatial and temporal cues that ultimately shape and organize neurogenic tissues. The developing vertebrate retina is a widely used model to study how these key signaling cascades modulate the mode and rate of division of neural progenitors, despite its complex three-dimensional architecture and the asymmetric differentiation dynamics. Here, we present a comprehensive multi-step framework that integrates in toto experiments with three dimensional image analysis and theoretical tools to provide a quantitative characterization of the dynamics of growth and differentiation of the developing vertebrate retina. Additionally, we use small molecule inhibitors to show that Hh and FGF activation promote differentiation and cell cycle pro- gression, while Wnt and Notch activation respectively increase and decrease the average division rate. These results represent a detailed and accurate quantitative characterization of the development and regulation of the vertebrate retina. We propose that the same framework can be directly used to characterize other in vitro or in vivo tissues.

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Section: Discussionmentioning

confidence: 54%

High-throughput three-dimensional characterization of morphogenetic signals during the formation of the vertebrate retina

Ledesma-Terrón,

Pérez-Dones,

Mazó-Durán

et al. 2024

Preprint

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Morphogenesis and Early Patterning

Marco-Ferreres,

Pajda-Szeligowska,

Bovolenta

2024

Reference Module in Neuroscience and Biobehavioral Psychology

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Timely neurogenesis enables increased nuclear packing order during neuronal lamination

Ferme,

Ryan,

Haase

et al. 2024

Preprint

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The coordination of cell proliferation, migration, and differentiation is crucial for organogenesis in many tissues, including the CNS and other organs that arise from pseudostratified epithelia (PSE). PSE feature densely packed elongated epithelial cells, with nuclei positioned along the apicobasal cell axis in a cell cycle-dependent manner. PSE serve as organ precursors in diverse developmental contexts across evolution. While the role of nuclear movements in PSE has been extensively studied, less is known about whether and how their nuclear packing arrangements and changes of packing state influence tissue morphogenesis. To address this, we analyzed nuclear shapes, sizes and neighborhood statistics by segmenting nuclei in 3D and over development in zebrafish retinal neuroepithelia (RNE). We find that in PSE nuclei exhibit orientational, nematic-like order but remain positionally disordered. This pattern is conserved in other, less packed, neuroepithelia, like the hindbrain, suggesting that nematic-like order is a hallmark of pseudostratification. Our analysis during retinal development also revealed that nuclear packing density increases, approaching theoretical packing limits for disordered monodisperse ellipsoids at stages when the tissue transitions to a laminated neuronal structure. As neurogenesis progresses, nuclear shapes are remodeled, enabling the RNE to shift to a crystalline, ordered structure, while maintaining orientational alignment. Failure to initiate neurogenesis results in severe tissue deformations due to increased buckling instability. Our results thus show an instance where nuclear shape and nuclear positioning and their changes are essential for proper retinal morphogenesis, a phenomenon most likely also found in other tissue arrangements.

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A Fish Eye View: Retinal Morphogenesis from Optic Cup to Neuronal Lamination

Cited by 4 publications

References 109 publications

High-throughput three-dimensional characterization of morphogenetic signals during the formation of the vertebrate retina

High-throughput three-dimensional characterization of morphogenetic signals during the formation of the vertebrate retina

Morphogenesis and Early Patterning

Timely neurogenesis enables increased nuclear packing order during neuronal lamination

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