Executive SummaryJuvenile fish passing through the turbines of hydro electric dams are exposed to numerous injurycausing mechanisms including both fluid and mechanical forces. Previous research has shown that these mechanisms cause similar-looking injuries to fish so most biological testing techniques have been inadequate for identifying specific causes of injury under different operating scenarios. Use of imaging technologies inside the turbines to observe the approach and interaction of fish with turbine structural elements has been proposed as one means for gaining a clearer understanding of causes of injury. In 2003 and 2004 Pacific Northwest National Laboratory conducted an investigation of imaging technologies that included theoretical and laboratory studies.The goal of this project was to identify and evaluate imaging technologies for observing juvenile fish within a Kaplan turbine, and specifically that would enable us to determine mechanisms of fish injury within an operating turbine unit. This evaluation documents the opportunities and constraints for observing juvenile fish at specific locations during turbine passage. These observations would be used to make modifications to dam structures and operations to improve conditions for fish passage while maintaining or improving hydropower production.The physical and hydraulic environment that fish experience as they pass through the turbines, including the physical structures of the intake, stay vanes, wicket gates, and runner, were studied and the regions with the greatest potential for injury were defined. Biological response data were also studied to determine the probable types of injuries sustained in the turbine intake and what types of injuries are detectable with imaging technologies. We grouped injury-causing mechanisms into two categories: fluid (pressure/cavitation, shear, turbulence) and mechanical (strike/collision, grinding/pinching, scraping). The physical constraints of the environment, together with the likely types of injuries to fish, provided the parameters needed for a rigorous imaging technology evaluation.Types of technology evaluated included both tracking and imaging systems using acoustic technologies (such as sonar and acoustic tags) and optic technologies (such as pulsed-laser videography, which is high-speed videography using a laser as the flash). Criteria for determining image data quality such as frame rate, target detectability, and resolution were used to quantify the minimum requirements of an imaging sensor. We based our calculations on the most demanding application --imaging head injuries to a subyearling Chinook salmon smolt passing through the runner tip region. Previous ex-situ and in-situ study results were used, along with the results from a laboratory experiment we conducted, to address tradeoffs in sensor capability in the typically low-visibility riverine environment. We concluded that a high-speed optical-imaging solution, such as pulsed-laser videography, was the only feasible technology to image fish fast e...