A five-miRNA signature predicts survival in gastric cancer using bioinformatics analysis

Zhang, Ziqiang; Dong, Yuanqiang; Jin, Hua; Xue, Hongyuan; Hu, Jian; Jiang, Tao; Li-ming, Shi; Du, Jianjun

doi:10.1016/j.gene.2019.02.058

Cited by 30 publications

(25 citation statements)

References 42 publications

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“…GC is among one of the most common malignancies globally and the prognosis remains unfavorable, in spite of the decreasing morbidity [10]. Interestingly, the deregulation of miRNAs in GC has been highlighted to play a role in tumorigenesis and metastasis [13,25]. Besides, the miRNA-mRNA network provides therapeutic and prognostic biomarkers for GC [26,27].…”

Section: Discussionmentioning

confidence: 99%

RETRACTED ARTICLE: MicroRNA-598 inhibits the growth and maintenance of gastric cancer stem-like cells by down-regulating RRS1

Yan

Wei

et al. 2019

Cell Cycle

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Emerging evidence has identified the critical role of microRNAs in gastric cancer (GC). Herein, this study intends to characterize the tumor suppressive role of microRNA-598 (miR-598) in GC stemlike cells, with the involvement of RRS1. The CD133+ GC stem-like cells were sorted by flow cytometry, after which immunofluorescence assay was used to determine the co-localization of CD133 and CD44v8-10. The miR-598 expression was examined in the CD133+ and CD133-cells. Subsequently, the CD133+ cells were subjected to miR-598 mimics, miR-598 inhibitors or RRS1 siRNA to validate the effect of miR-598 on GC stem-like cell proliferation, colony formation, apoptosis, migration and invasion capacities. Besides, the effect of miR-598 on the expression of key factors (OCT4, SOX2 and NANOG) associated with stem cell characteristics was measured. The obtained results indicated that the sphere forming capacity was higher in CD133+ cells. CD133 + MKN-45 cells expressed CD133 and CD44v8-10, and were expressed on the cell membrane. MiR-598 was poorly expressed in CD133+ cells. Notably, miR-598 negatively regulated RRS1. In response to miR-598 mimics and RRS1 siRNA, the MKN-45 cells displayed inhibited proliferation, colony formation, migration and invasion, accompanied by elevated apoptosis. Besides, the miR-598 inhibitors reversed the situation. This study highlights that miR-598 a tumor suppressor in GC stem-like cells by inhibiting RRS1, whereby miR-598 represses MKN-45 cell growth and invasion by attenuating self-renewal of GC stem-like cells.

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Section: Discussionmentioning

confidence: 99%

RETRACTED ARTICLE: MicroRNA-598 inhibits the growth and maintenance of gastric cancer stem-like cells by down-regulating RRS1

Yan

Wei

et al. 2019

Cell Cycle

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“…changes in mirna expression contribute to the initiation and progression of cancer (45,46). The association between mirnas and tumors suggests that mirnas may be altered in patients with gastric cancer (47,48). a previous study demonstrated that mir-218 increased chemosensitivity to cisplatin in vitro and in vivo by inducing apoptosis (18).…”

Section: Discussionmentioning

confidence: 98%

Differential expression profile analysis of cisplatin‑regulated miRNAs in a human gastric cancer cell line

et al. 2019

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cisplatin, one of the most commonly used drugs in combination chemotherapy, is an effective anti-tumor agent widely used for diverse tumor types. Micrornas (mirnas/mirs) are involved in the occurrence, development, diagnosis and treatment of cancer. Therefore, the aim of the current study was to explore whether cisplatin exerts anticancer effects by causing differential expression of mirnas in human gastric cancer cells. The human gastric cancer cell line nci-n87 was cultured with a certain dose of cisplatin and high-throughput sequencing combined with reverse transcription-quantitative polymerase chain reaction (rT-qPcr) was performed to detect cisplatin-regulated mirnas. mirnas upregulated and downregulated following cisplatin exposure were analyzed. High-throughput sequencing revealed 33 upregulated and 16 downregulated miRNAs. A total of five significantly upregulated and five significantly downregulated mirnas were identified by rT-qPcr. The expression levels of hsa-mir-1246 and hsa-mir-892b were consistent with the results obtained from high-throughput sequencing. Gene ontology and Kyoto encyclopedia of Genes and Genomes pathway clustering of cisplatin-regulated mirnas revealed that the mirnas regulated genes involved in several biological processes and signaling pathways. The results obtained in the current study suggested that cisplatin may exert an important anticancer effect in gastric cancer via complex biological processes and signaling pathways.

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“…Huang et al used clinicopathologic risk factors to build the radiomics signature and radiomics nomogram for preoperative prediction of lymph node metastasis in colorectal cancer, which can conveniently improve the preoperative individualized prediction. Zhang et al used high‐throughput microRNA data in TCGA database to obtain a 5‐microRNA signature for predicting survival time of gastric cancer patients. A 6‐gene signature was identified by using univariate Cox regression analysis and the LASSO‐Cox regression model to predict overall survival for hepatocellular carcinoma .…”

Section: Discussionmentioning

confidence: 99%

Immune cell infiltration as a biomarker for the diagnosis and prognosis of digestive system cancer

et al. 2019

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The digestive system cancers are aggressive cancers with the highest mortality worldwide. In this study, we undertook a systematic investigation of the tumor immune microenvironment to identify diagnostic and prognostic biomarkers. The fraction of 22 immune cell types of patients were estimated using CIBERSORT. The least absolute shrinkage and selection operator (LASSO) analysis was carried out to identify important immune predictors. By comparing immune cell compositions in 801 tumor samples and 46 normal samples, we constructed the diagnostic immune score (DIS), showing high specificity and sensitivity in the training (area under the receiver operating characteristic curve [AUC] = 0.929), validation (AUC = 0.935), and different cancer type cohorts (AUC > 0.70 for all).We also established the prognostic immune score (PIS), which was an effective prognostic factor for relapse-free survival in training, validation, and entire cohorts (P < .05). In addition, PIS provided a higher net benefit than TNM stage. A composite nomogram was built based on PIS and patients' clinical information with well-fitted calibration curves (c-index = 0.84). We further used other cohorts from Gene Expression Omnibus databases and obtained similar results, confirming the reliability and validity of the DIS and PIS. In addition, the unsupervised clustering analysis using immune cell proportions revealed 6 immune subtypes, suggesting that the immune types defined as having relatively high levels of M0 or/and M1 macrophages were the high-risk subtypes of relapse. In conclusion, this study comprehensively analyzed the tumor immune microenvironment and identified DIS and PIS for digestive system cancers. K E Y W O R D Sdiagnosis, digestive system cancer, immune cell, prognosis, TCGA

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A five-miRNA signature predicts survival in gastric cancer using bioinformatics analysis

Cited by 30 publications

References 42 publications

RETRACTED ARTICLE: MicroRNA-598 inhibits the growth and maintenance of gastric cancer stem-like cells by down-regulating RRS1

RETRACTED ARTICLE: MicroRNA-598 inhibits the growth and maintenance of gastric cancer stem-like cells by down-regulating RRS1

Differential expression profile analysis of cisplatin‑regulated miRNAs in a human gastric cancer cell line

Immune cell infiltration as a biomarker for the diagnosis and prognosis of digestive system cancer

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