Increasing interest in grape (Vitis sp.) and wine production in the upper midwest region of the United States has created a need for science-based information that characterizes the potential of cold-climate cultivars to produce quality grapes with acceptable yields. We evaluated the yield and quality (composition) of grapes from 12 cold-climate, interspecific-hybrid grape cultivars (northern hybrids) grown in a randomized and replicated field plot in central Iowa. The grape trial was planted in 2008, and crop performance of cultivars was evaluated from 2012 through 2017 (yield) and 2014 through 2017 (berry composition). The trial included two established cultivars, five newer cultivars, and five advanced selections. The established cultivars included in the study as controls were Frontenac and St. Croix. The newer cultivars evaluated in this study were Arandell, Corot Noir, La Crescent, Marquette, and Petit Ami, and the advanced selections were MN 1189, MN 1200, MN 1220, MN 1235, and MN 1258. Yield and productivity were characterized by measuring yield per vine, number of clusters per vine, average cluster weight, and pruning weight. The fruit composition indices were soluble solids concentration (SSC), pH, titratable acidity (TA), and sugar:acid ratio (SSC ÷ TA). On the basis of their strong results for both yield and fruit composition measures, ‘Marquette’, MN 1235, and MN 1220 ranked as the top-performing cultivars in Iowa’s climate, followed by Petit Ami and St. Croix. ‘Petit Ami’ had slightly lower yield consistency and slightly lower results for SSC than did the top performing cultivars, and St. Croix had among the highest and most consistent yields of the trial but showed lower results for SSC and sugar:acid ratio than many of the other cultivars. ‘La Crescent’ had midrange yields and high SSC, but the high TA of ‘La Crescent’ fruit resulted in a low sugar:acid ratio at harvest. Two cultivars (MN 1258 and MN 1200) had relatively low yields in Iowa’s climate but achieved good results for composition indices. ‘Frontenac’ had high, consistent yields and achieved high SSC, but the very high TA of ‘Frontenac’ fruit resulted in a very low sugar:acid ratio compared with most other cultivars. The remaining three cultivars (Corot Noir, MN 1189, and Arandell) performed poorly in Iowa’s climate, showing both low yield and undesirable fruit composition indices compared with the other cultivars in the trial. An itemized summary of the relative ratings for yield and fruit composition is provided to aid growers in selection and management of grape cultivars for use in Iowa and other areas of similar climate.