A flagellar A-kinase anchoring protein with two amphipathic helices forms a structural scaffold in the radial spoke complex

Sivadas, Priyanka; Dienes, Jennifer M.; Maurice, Martin St.; Meek, William D.; Yang, Pinfen

doi:10.1083/jcb.201111042

Cited by 30 publications

(42 citation statements)

References 67 publications

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“…In agreement with the previous studies (Diener et al, 1993;Patel-King et al, 2004;Sivadas et al, 2012), RSP3 appears to be localized all the way from the RS head to the root of the RS stalk, and one spoke contains at least two copies of RSP3 (Figs. 2 and S1; Wirschell et al, 2008;Diener et al, 2011).…”

Section: Architecture Of Rssupporting

confidence: 80%

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Mechanosignaling between central apparatus and radial spokes controls axonemal dynein activity

Oda¹,

Yanagisawa²,

Yagi³

et al. 2014

J Gen Physiol

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Section: Architecture Of Rssupporting

confidence: 80%

“…2), a distribution that is unique among RSPs and is consistent with the previous deletion mutant analysis of RSP3 (Sivadas et al, 2012). RSP11 has sequence similarity to the regulatory subunit (RIIa) of cAMP-dependent protein kinase (PKA), and it has been proposed to regulate flagellar beating through interaction with RSP3 .…”

Section: Architecture Of Rssupporting

confidence: 65%

Mechanosignaling between central apparatus and radial spokes controls axonemal dynein activity

Oda¹,

Yanagisawa²,

Yagi³

et al. 2014

J Gen Physiol

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“…RII overlay and deletion mutation analysis have demonstrated that RSP3 is an A-kinase anchoring protein (AKAP) located near the inner dynein arm (Gaillard et al 2001(Gaillard et al , 2006. AKAPs are a well-characterized class of scaffolding proteins that bind to PKA holoenzyme to define its locations within the cell (Sivadas et al 2012;Taylor et al 2004). RSP3 acts as an AKAP to control dynein activity by regulating axonemal PKA, a ubiquitous protein composed of two catalytic subunits and two regulatory subunits, which is involved in numerous regulatory events in the cell.…”

Section: Introductionmentioning

confidence: 98%

Subcellular localization and function of mouse radial spoke protein 3 in mammalian cells and central nervous system

et al. 2014

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Radial spoke protein 3 (RSP3) was first identified in Chlamydomonas as a component of the radial spoke. The mammalian homologue of the Chlamydomonas RSP3 gene is mainly expressed in testis and developing central nervous system (CNS). However, the subcellular localization and function of mammalian RSP3 in the developing brain and mammalian cells remain poorly understood. Here we show that the mouse RSP3 accumulates at the perinuclear region of Chinese hamster ovary (CHO) and 293T cells. Detailed analysis shows that the mouse RSP3 is not co-localized with the endoplasmic reticulum or Golgi apparatus markers in CHO cells. Using in utero electroporation, we found that over-expression of mammalian RSP3 increases the percentage of neurons reaching the upper cortical plate. In vivo analysis shows that the mouse RSP3 mainly accumulates in the proximal cytoplasmic dilation of the leading process of the migrating cortical neurons. Furthermore, we find that the mammalian RSP3 concentrates in the ependymal cilia as a component of the cilia. Thus, our data provide the first evidence for the subcellular localization and function of mammalian RSP3 in mammalian cells and developing CNS.

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“…AKAP is a well-characterized class of scaffolding proteins that bind to PKA holoenzyme at defined locations within the cells (Sivadas et al 2012;Taylor et al 2004). These findings imply the crucial role of RSP3 in cilia and flagella motility and signaling transduction.…”

Section: Introductionmentioning

confidence: 95%

“…Keywords RSP3 · Nucleocytoplasmic shuttling · In utero electroporation · Neurogenesis · Cilia dynein activity by regulating axonemal cAMP-dependent protein kinase (PKA) (Gaillard et al 2001(Gaillard et al , 2006Sivadas et al 2012). AKAP is a well-characterized class of scaffolding proteins that bind to PKA holoenzyme at defined locations within the cells (Sivadas et al 2012;Taylor et al 2004).…”

Section: Introductionmentioning

confidence: 99%

The mouse radial spoke protein 3 is a nucleocytoplasmic shuttling protein that promotes neurogenesis

Ren

Xiu

Zhang

et al. 2015

Histochem Cell Biol

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Radial spoke protein 3 (RSP3) was first identified in Chlamydomonas as a component of radial spoke, which is important for flagellar motility. The mammalian homolog of the Chlamydomonas RSP3 protein is found to be a mammalian protein kinase A-anchoring protein that binds ERK1/2. Here we show that mouse RSP3 is a nucleocytoplasmic shuttling protein. The full-length RSP3-EGFP fusion protein is mainly located in the cytoplasm of Chinese hamster ovary cells. However, by using deletion mutants of RSP3, we identified two nuclear localization signals and a nuclear export signal in RSP3. Moreover, using in utero electroporation, we found that overexpression of RSP3 in the developing cerebral cortex promotes neurogenesis. The layer II/III of the neocortex was much thicker in the RSP3-transfected region than that of the untransfected region in the neocortex. We also show that RSP3 is specifically located in the primary cilia of the radial glial cells, where it acts as a signaling mediator that regulates neurogenesis. Thus, our results suggest that RSP3 is a nucleocytoplasmic shuttling protein and plays an essential role in neurogenesis.

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A flagellar A-kinase anchoring protein with two amphipathic helices forms a structural scaffold in the radial spoke complex

Abstract: Amphipathic helices in the A-kinase anchoring protein RSP3 bind to spoke proteins involved in the assembly and modulation of the flagellar radial spoke complex, expanding the repertoire of these versatile helical protein motifs.

Cited by 30 publications

References 67 publications

Mechanosignaling between central apparatus and radial spokes controls axonemal dynein activity

Mechanosignaling between central apparatus and radial spokes controls axonemal dynein activity

Subcellular localization and function of mouse radial spoke protein 3 in mammalian cells and central nervous system

The mouse radial spoke protein 3 is a nucleocytoplasmic shuttling protein that promotes neurogenesis

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