A flap endonuclease 1-assisted universal viral nucleic acid sensing system using surface-enhanced Raman scattering

Abstract

Flap endonuclease 1 recognizes a specific DNA structure and cleaves Raman tag-labeled probe molecules in a target-specific manner. With SERS-based sensing, the developed detection approach produces sensitive, quantitative, and multiplexable signals.

Tip‐based Lithography with a Sacrificial Layer

“…In detail, N bulk and N SERS were calculated using the following equations: [50][51][52] N bulk = 𝜌S spot hN A ∕M R6G…”

“…In detail, N bulk and N SERS were calculated using the following equations: [ 50–52 ] $$$$\begin{equation}{{\mathrm{N}}}_{{\mathrm{bulk}}} = {\mathrm{\rho S}}_{{\mathrm{spot}}}{\mathrm{hN}}_{\mathrm{A}}/{{\mathrm{M}}}_{{\mathrm{R}}6{\mathrm{G}}}\end{equation}$$$$ $$$$\begin{equation}{{\rm{N}}}_{{\rm{SERS}}} = {\rm{CV}}{{\rm{N}}}_{\rm{A}}{{\rm{S}}}_{{\rm{spot}}}/{{\rm{S}}}_{{\rm{diffusion}}}\end{equation}$$$$where N bulk /N SERS is represented as follows: $$$$\begin{equation}{{\mathrm{N}}}_{{\mathrm{bulk}}}/{{\mathrm{N}}}_{{\mathrm{SERS}}} = {\mathrm{\rho S}}_{{\mathrm{diffusion}}}{\mathrm{h}}/{\mathrm{CVM}}_{{\mathrm{R}}6{\mathrm{G}}}\end{equation}$$$$…”