A flow cytometric approach to assess phytoplankton respiration

Grégori, Gérald; Denis, Michel; Lefèvre, D.; Beker, Béatriz

doi:10.1007/978-94-017-0623-0_15

Cited by 10 publications

(9 citation statements)

References 26 publications

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“…Esterase activity of microalgae exposed to the NPs was evaluated using non-fluorescent lipophilic dye fluorescein diacetate (FDA) [65,66]. Membrane potential of microalgae cells was assessed by a lipophilic, positively charged fluorescent dye 3,3 -dihexyloxacarbocyanine iodide (DiOC 6 ) [67,68]. To determine the size of microalgae cells, a size calibration kit, batch F13838 (Molecular probes, Eugene, OR, USA) with the certified size distribution of 1, 2, 4, 6, 10, 15 µm was used for the forward scatter emission channel.…”

Section: Flow Cytometrymentioning

confidence: 99%

Comparison of the Level and Mechanisms of Toxicity of Carbon Nanotubes, Carbon Nanofibers, and Silicon Nanotubes in Bioassay with Four Marine Microalgae

et al. 2020

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Nanoparticles (NPs) have various applications in medicine, cosmetics, optics, catalysis, environmental purification, and other areas nowadays. With an increasing annual production of NPs, the risks of their harmful influence to the environment and human health is rising. Currently, our knowledge about the mechanisms of interaction between NPs and living organisms is limited. Additionally, poor understanding of how physical and chemical characteristic and different conditions influence the toxicity of NPs restrict our attempts to develop the standards and regulations which might allow us to maintain safe living conditions. The marine species and their habitat environment are under continuous stress due to anthropogenic activities which result in the appearance of NPs in the aquatic environment. Our study aimed to evaluate and compare biochemical effects caused by the influence of different types of carbon nanotubes, carbon nanofibers, and silica nanotubes on four marine microalgae species. We evaluated the changes in growth-rate, esterase activity, membrane polarization, and size changes of microalgae cells using flow cytometry method. Our results demonstrated that toxic effects caused by the carbon nanotubes strongly correlated with the content of heavy metal impurities in the NPs. More hydrophobic carbon NPs with less ordered structure had a higher impact on the red microalgae P. purpureum because of higher adherence between the particles and mucous covering of the algae. Silica NPs caused significant inhibition of microalgae growth-rate predominantly produced by mechanical influence.

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Section: Flow Cytometrymentioning

confidence: 99%

Comparison of the Level and Mechanisms of Toxicity of Carbon Nanotubes, Carbon Nanofibers, and Silicon Nanotubes in Bioassay with Four Marine Microalgae

et al. 2020

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“…In aquatic ecology, FCM is routinely employed for determination of abundance, viability, and activity of viruses (Marie et al 1999), bacteria (Bernard et al 2001;Bunthof et al 1999;Lebaron, Catala, and Parthuisot 1998;Lebaron et al 2001;Mortimer, Mason, and Gant 2000;Roth et al 1997;Williams et al 1998), phytoplankton (Grégori et al 2002;Marie et al 1997;Marie et al 2000;Olson, Zettler, and Durand 1993), and planktonic protozoans (Lindström, Weisse, and Stadler 2002;Parrow and Burkholder 2002;Wong and Whiteley 1996). Rapid and reliable FCM methods have also been developed to enumerate and to evaluate growth, viability, infectivity, and metabolic activities of gastroenteric protozoan parasites, such as Cryptosporidium spp.…”

mentioning

confidence: 99%

Assessment of the Cell Viability of Cultured Perkinsus marinus (Perkinsea), a Parasitic Protozoan of the Eastern Oyster, Crassostrea virginica, Using SYBRgreen–Propidium Iodide Double Staining and Flow Cytometry

Soudant

Chu

Lund

2005

J Eukaryotic Microbiology

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A flow cytometry (FCM) assay using SYBRgreen and propidium iodide double staining was tested to assess viability and morphological parameters of Perkinsus marinus under different cold- and heat-shock treatments and at different growth phases. P. marinus meront cells, cultivated at 28 degrees C, were incubated in triplicate for 30 min at -80 degrees C, -20 degrees C, 5 degrees C, and 20 degrees C for cold-shock treatments and at 32 degrees C, 36 degrees C, 40 degrees C, 44 degrees C, 48 degrees C, 52 degrees C, and 60 degrees C for heat-shock treatments. A slight and significant decrease in percentage of viable cells (PVC), from 93.6% to 92.7%, was observed at -20 degrees C and the lowest PVC was obtained at -80 degrees C (54.0%). After 30 min of heat shocks at 40 degrees C and 44 degrees C, PVC decreased slightly but significantly compared to cells maintained at 28 degrees C. When cells were heat shocked at 48 degrees C, 52 degrees C, and 60 degrees C heavy mortality occurred and PVC decreased to 33.8%, 8.0%, and 3.4%, respectively. No change in cell complexity and size was noted until cells were heat shocked at >or=44 degrees C. High cell mortality was detected at stationary phase of P. marinus cell culture. Cell viability dropped below 40% in 28-day-old cultures and ranged 11-25% in 38 to 47-day-old cultures. Results suggest that FCM could be a useful tool for determining viability of cultured P. marinus cells.

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“…To determine the optimal concentration of fluorescent dyes and the optimal duration of staining, a series of preliminary measurements were made for each microalgae species before the assessment of growth-inhibition and membrane potential of cells [ 59 ]. The exposure periods of 24 and 72 h were selected according to the standard methods commonly used to assess the toxicity of test substances and materials in aqueous systems with microalgae [ 63 , 64 ].…”

Section: Methodsmentioning

confidence: 99%

Aquatic toxicity of particulate matter emitted by five electroplating processes in two marine microalgae species

et al. 2021

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A flow cytometric approach to assess phytoplankton respiration

Cited by 10 publications

References 26 publications

Comparison of the Level and Mechanisms of Toxicity of Carbon Nanotubes, Carbon Nanofibers, and Silicon Nanotubes in Bioassay with Four Marine Microalgae

Comparison of the Level and Mechanisms of Toxicity of Carbon Nanotubes, Carbon Nanofibers, and Silicon Nanotubes in Bioassay with Four Marine Microalgae

Assessment of the Cell Viability of Cultured Perkinsus marinus (Perkinsea), a Parasitic Protozoan of the Eastern Oyster, Crassostrea virginica, Using SYBRgreen–Propidium Iodide Double Staining and Flow Cytometry

Aquatic toxicity of particulate matter emitted by five electroplating processes in two marine microalgae species

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