A flow cytometric method for assessing viability of intraerythrocytic hemoparasites

Wyatt, Carol R.; Goff, Will L.; Davis, William C.

doi:10.1016/0022-1759(91)90122-v

Cited by 100 publications

(76 citation statements)

References 14 publications

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“…Flow cytometry is able to detect earlier infections but also requires a specific fluorescent dye, e.g. hydroethidine, to stain live parasites present in intact erythrocytes (Wyatt et al, 1991). Other diagnostic methods like, e.g.…”

Section: Introductionmentioning

confidence: 99%

Label-free detection of Babesia bovis infected red blood cells using impedance spectroscopy on a microfabricated flow cytometer

et al. 2007

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Impedance spectroscopy is a powerful tool for label-free analysis and characterisation of living cells. In this work, we achieved the detection of Babesia bovis infected red blood cells using impedance spectroscopy on a microfabricated flow cytometer. The cellular modifications caused by the intracellular parasite result in a shift in impedance which can be measured dielectrically. Thus, a rapid cell-by-cell detection with microliter amounts of reagents is possible. Unlike other diagnostic tests, this method does not depend on extensive sample pre-treatment or expensive chemicals and equipment.

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Section: Introductionmentioning

confidence: 99%

Label-free detection of Babesia bovis infected red blood cells using impedance spectroscopy on a microfabricated flow cytometer

et al. 2007

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“…Thus, if the parasite is inside the cell, its DNA can be stained with specific nucleic acid-binding dyes and detected by flow cytometry (FCM). Different dyes, such as Hoechst 33258 (5), acridine orange (6), thiazole orange (7), or hydroethidine (8), have been considered for the determination of parasitemia in cultures of P. falciparum by FCM. Jacobberger et al (9) used DiOC1, a membrane potential respon-sive dye and Hoechst 33342 to evaluate parasitemia levels in mice (7).…”

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confidence: 99%

Development and validation of flow cytometric measurement for parasitemia in cultures of P. falciparum vitally stained with YOYO‐1

Gerena

Xie

et al. 2007

Cytometry Pt A

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Background: The need for improved malaria diagnostics has long been recognized. Methods: Human parasitized erythrocytes based on the principles of flow cytometry (FCM) method is described for the determination of parasitemia in Plasmodium falciparum cultures using the fluorescence activated cell sorter and DNA-binding fluorescent dye, YOYO-1. The same assay samples can be also evaluated both microscopically and by scintillation counting after use of 3 H-hypoxanthine-labeled parasites. Results: The counts of uninfected, infected, and nucleated cells occurred with high precision. The cells were categorized into different populations according to their physical or chemical properties such as RNase treatment and compensation required optimization. The detection and quantitation limits in the assay were 0.003% and 0.008% parasitemia, respectively. Overall, the parasite counts by FCM measurement correlated highly (r 2 5

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“…Different dyes, such as Hoechst 33258 (Brown, 1980), acridine orange (Whaun, 1983), thiazole orange (Makler, 1987), or hydroethidine (Wyatt, 1991), have been considered for the determination of parasitemia in cultures of P. falciparum by FCM. et al (1984) used DiOC1, a membrane potential responsive dye and Hoechst 33342 to evaluate parasitemia levels in mice (Makler, 1987).…”

Section: Parasitesmentioning

confidence: 99%

Applications of Flow Cytometry to Clinical Microbiology

Pieretti¹,

Masucci²,

Moretti³

2012

Clinical Flow Cytometry - Emerging Applications

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A flow cytometric method for assessing viability of intraerythrocytic hemoparasites

Cited by 100 publications

References 14 publications

Label-free detection of Babesia bovis infected red blood cells using impedance spectroscopy on a microfabricated flow cytometer

Label-free detection of Babesia bovis infected red blood cells using impedance spectroscopy on a microfabricated flow cytometer

Development and validation of flow cytometric measurement for parasitemia in cultures of P. falciparum vitally stained with YOYO‐1

Applications of Flow Cytometry to Clinical Microbiology

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