A flow cytometry method for quantitative measurement and molecular investigation of the adhesion of bacteria to yeast cells
Marion Schiavone,
Adilya Dagkesamanskya,
Pierre-Gilles Vieu
et al.
Abstract:The interaction of bacteria with yeasts deserves great interest in animal and human welfare. To investigate these microbial interactions at the cellular and molecular level, a simple, reliable and quantitative method is proposed. We showed that flow cytometry enables the interaction to be measured at single cell level,by distinguishing single yeast cells with fluorescent bound bacteria from cell aggregates. free fluorescent bacteria Flow cytometry coupled with imaging showed that number of bacteria attached to… Show more
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