A fluorescence aptasensor based on two-dimensional sheet metal-organic frameworks for monitoring adenosine triphosphate

Hai, Xiaoman; Li, Nan; Wang, Ke; Zhang, Zhiqi; Zhang, Jing; Dang, Fuquan

doi:10.1016/j.aca.2017.10.028

Cited by 44 publications

(15 citation statements)

References 63 publications

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“…The 2D MOF nanosheets have been widely used in the construction of FRET sensors in nanomedicine as they exhibit several advantages over other 2D nanomaterials such as high structural tunability and degradability [148][149][150][151][152][153]. In the study of Wang et al [149], the two-color intracellular adenosine imaging in living cells was achieved by using the lanthanide-based MOF nanosheets.…”

Section: Other 2d Nanomaterials As the Acceptor In Fret Sensorsmentioning

confidence: 99%

Two-dimensional nanomaterials for Förster resonance energy transfer–based sensing applications

Zhou

Chen

et al. 2020

Nanophotonics

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Abstract Förster resonance energy transfer (FRET)–based sensing has been steadily gaining popularity in the areas of biochemical analysis, environmental monitoring, and disease diagnosis in the past 20 years. Two-dimensional (2D) nanomaterials are extensively used as donors and acceptors in the FRET sensing because of their attractive optical and chemical properties. In this review, we first present the FRET theory and calculations to give readers a better understanding of the FRET phenomenon. Then, we discuss the recent research advances in using 2D nanomaterials as donors and acceptor in FRET sensing. Finally, we summarize the existing challenges and future directions of 2D nanomaterials in the FRET sensing applications.

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Section: Other 2d Nanomaterials As the Acceptor In Fret Sensorsmentioning

confidence: 99%

Two-dimensional nanomaterials for Förster resonance energy transfer–based sensing applications

Zhou

Chen

et al. 2020

Nanophotonics

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“…Compared with signal amplification strategies used in above literatures, suppressing the background signal strategies can design sensor with a simpler structure and needs a shorter reaction time (Liu et al 2015;Lv et al 2015). The quencher, such as gold nanoparticles (Jin et al 2009;Yin et al 2010), graphene oxide (GO) (Liu et al 2015;Lv et al 2015;Luo et al 2018), metal-organic frameworks (Hai et al 2018), and magnetic nanoparticles (Saberi et al 2018), can inhibit the background signal to increases sensor sensitivity. But these approaches usually involve fluorescence-labeled probe, resulting in relatively high cost of detection.…”

Section: Introductionmentioning

confidence: 99%

A label-free logic gate hairpin aptasensor for sensitive detection of ATP based on graphene oxide and PicoGreen dye

Zhang

et al. 2021

J Anal Sci Technol

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Background In this paper, a simple, enzyme-free, label-free fluorescence, high sensitivity logic gate hairpin aptasensor was developed for adenosine triphosphate (ATP) detection based on graphene oxide (GO) and PicoGreen dye. Methods Using single-strand deoxyribonucleic acid (DNA) and adenosine triphosphate (ATP) as input signal and fluorescence signal as output signal, if single-strand DNA (DNA-L), single-strand DNA (DNA-S), and ATP were present at the same time, one segment of DNA-L formed a hairpin ring with ATP, and the other segment of DNA-L formed a completely complementary hairpin stem with DNA-S. The hairpin DNA was detached from the GO surface, and PicoGreen dye was embedded into the hairpin stem, and the fluorescence signal was enhanced. The molecular logic gate was constructed through the establishment of logic histogram, logic circuit, truth table, and logic formula. The biosensor-related performances including sensitivity, selectivity, and linearity were investigated, respectively. Results We have successfully constructed a AND logic gate. The detection limit of ATP is 138.0 pmol/L (3σ/slope) with detection range of 50–500 nmol/L (R2 = 0.98951), and its sensitivity is 4.748 × 106–6.875 × 108 a.u. (mol/L)−1. Conclusions The logic gate hairpin aptamer sensor has the advantages of high sensitivity, low detection limit, and low cost, and can be successfully applied to the detection of adenosine triphosphate (ATP) in actual human urine samples.

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“…Target recognition induces conformational change in the aptamer structure leading to enhancement or quenching of the fluoresce (case A) or detachment of one or both complementary sequences (case B), resulting in the recovery of the quenched fluorescent signals [26]. Alternatively, the fluorescence of target-free and fluorophore-labeled aptamer can be quenched by the non-specific adsorption into a surface such as; graphene oxide sheets [27], metal organic frame work [28], or nanoparticles [29]. Target recognition leads to the dissociation of the adsorbed aptamer and enhancement of the fluorescent signals.…”

Section: Introductionmentioning

confidence: 99%

Label-Free Fluorescent Aptasensor for Small Targets via Displacement of Groove Bound Curcumin Molecules

Alyamani

Alsager

Zourob

2019

Sensors

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Signal transduction based on fluorescence is one of the most common optical aptasensors for small molecules. Sensors with a number of unique features including high sensitivity, low cost, and simple operation can be constructed easily. However, the label-free fluorescent approach is limited to synthetic dyes that bind strongly to the aptamer sequence and result in a diminished sensor operation with high detection limits. In this study, we report the use of curcumin as a fluorescent probe to signal aptamer/small target binding events. A substantial enhancement in curcumin’s fluorescent emission was observed when bound into the grooves of vitamin D3 (VTD3) binding aptamer, as an example. However, the introduction of the target molecule causes the aptamer to undergo a conformational change that favors complexing the target molecule over binding the curcumin dye. The sensor was able to detect VTD3 down to 1 fM concentration in buffer solutions and extracted blood samples, operate at a wide dynamic range, and discriminate against potential biological interfering molecules including VTD2. The operation of the curcumin based fluorescent sensor is at least six orders of magnitude more sensitive than a VTD3 sensor constructed with the synthetic dye SYBR Green I. The generality of the reported label-free approach was applied with a previously isolated 75-mer bisphenol-A (BPA) aptamer, confirming that the reported sensing strategy is not confined on a particular aptamer sequence. Our work not only reports a novel sensor format for the detection of small molecules, but also serves fluorescent sensor’s most pressing need being novel fluorophores for multiplex targets detection.

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A fluorescence aptasensor based on two-dimensional sheet metal-organic frameworks for monitoring adenosine triphosphate

Cited by 44 publications

References 63 publications

Two-dimensional nanomaterials for Förster resonance energy transfer–based sensing applications

Two-dimensional nanomaterials for Förster resonance energy transfer–based sensing applications

A label-free logic gate hairpin aptasensor for sensitive detection of ATP based on graphene oxide and PicoGreen dye

Label-Free Fluorescent Aptasensor for Small Targets via Displacement of Groove Bound Curcumin Molecules

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