A fluorescence AuNPs-LISA: A new approach for Opisthorchis viverrini (Ov) antigen detection with a simple fluorescent enhancement strategy by surfactant micelle in urine samples

Taron, Wichit; Jamnongkan, Wassana; Phetcharaburanin, Jutarop; Klanrit, Poramate; Namwat, Nisana; Techasen, Anchalee; Sithithaworn, Paiboon; Khuntikeo, Narong; Boonmars, Thidarut; Loilome, Watcharin; Ngeontae, Wittaya

doi:10.1016/j.saa.2021.119633

Cited by 10 publications

(4 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Nanoparticles, magnetic particles, specific nucleic acid probes, enzyme cycling and photonic thermocycling platforms have been merged with the conventional systems to improve their detection limits and reduce the turnaround time. 55,57,58,63,81,83 While being independent of thermocycling, exhibiting equal or better accuracy and reducing the time required for results, isothermal amplification-based nucleic acid detection platforms have been developed. [92][93][94][97][98][99][100]105,112,[115][116][117]120 Even though the traditional techniques have evolved, they are still not able to reach the most vulnerable populations due to their stringent requirement of working conditions and need for advanced infrastructure.…”

Section: Discussionmentioning

confidence: 99%

“…A 1200-fold enhanced detection sensitivity was observed when compared with colorimetric AuNPs-LISA. 58 To increase the reach of this platform to places where infection with Opisthorchis viverrini is common, a smartphone-based portable fluorometer was designed to be used as a POC device for diagnosis of OvAg in urine samples. 59 Wu et al , 2017 were able to develop an enhanced fluorescence ELISA system which utilized human alpha-thrombin triggering fluorescence turn-on signals.…”

Section: Molecular Diagnosticsmentioning

confidence: 99%

See 1 more Smart Citation

Diagnosis of infectious diseases: complexity to convenience

Syed,

Rahaman,

Mondal

et al. 2024

Sens. Diagn.

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Molecular Diagnosticsmentioning

confidence: 99%

Diagnosis of infectious diseases: complexity to convenience

Syed,

Rahaman,

Mondal

et al. 2024

Sens. Diagn.

View full text Add to dashboard Cite

show abstract

“…Lastly, the main drawback of urine assay by ELISA is that it takes time to complete and needs experienced operators and special equipment. Adaptation of the urine assay for rapid-test platforms [ 31 , 32 ], including rapid diagnostic tests, is needed for large scale screening of opisthorchiasis.…”

Section: Discussionmentioning

confidence: 99%

Effects of day-to-day variation of Opisthorchis viverrini antigen in urine on the accuracy of diagnosing opisthorchiasis in Northeast Thailand

Worasith

Wongphutorn²,

Homwong³

et al. 2022

PLoS ONE

View full text Add to dashboard Cite

Antigen detection in urine using an enzyme-linked immunosorbent assay (ELISA) is more sensitive than fecal examination for diagnosis of opisthorchiasis and for assessment of the effects of drug treatment. It is not known whether day-to-day variation of urine composition, including levels of Opisthorchis viverrini antigen, influences the urine assay. We investigated this topic with the cooperation of participants from two localities in Northeast Thailand. Project participants were screened for parasite infections for three consecutive days using the quantitative formalin-ethyl acetate concentration technique (FECT) to detect O. viverrini eggs and the urine ELISA for detection of O. viverrini antigen. A subset of participants (n = 801) with matched fecal and urine samples were analyzed for comparison of inter-day prevalence estimates and the performance of the urine assay compared against FECT for diagnosis of opisthorchiasis. The daily prevalence measured by the urine assay ranged between 29.0%-30.2% while those by FECT ranged between 11.9%-20.2%. The cumulative three-day prevalence estimate determined by the urine antigen assay was 30.3%, which was significantly higher than that by FECT (20.2%, p < 0.05). A significant positive correlation was found between the concentration of antigen in urine and fecal egg counts (p < 0.001). Overall, the urine assay had better diagnostic performance for opisthorchiasis than fecal examination by FECT. The high sensitivity plus negligible daily variation of O. viverrini antigen in urine indicates the utility of the urine assay for diagnosis, as well as population screening, of opisthorchiasis.

show abstract

“…The concentrations of Opisthorchis viverrini antigen in urine samples is so low that the sensitivity of colorimetric AuNPs‐based assays cannot meet the requirement of detecting the early‐stage liver fluke infection. To address this issue, the same research group [ 97 ] further developed an AuNPs‐based FLEIA by substituting TMB with o‐phenylenediamine. The fluorescence assay could detect Opisthorchis viverrini antigen in the range from 34.18 to 273.44 ng mL –1 with a LOD of 36.97 ng mL –1 , a 1200‐fold higher sensitivity than the colorimetric one.…”

Section: Nanozyme‐implemented Immunoassaysmentioning

confidence: 99%

Nanobody and Nanozyme‐Enabled Immunoassays with Enhanced Specificity and Sensitivity

Wang

Xianyu

2022

Small Methods

View full text Add to dashboard Cite

treatment of cancers, the second leading cause of death in the world that are hard to be treated in the terminal stages. [2] For instance, liver cancer is one of the most lethal cancers and has a poor prognosis with a 5-year survival rate of less than 20%. [3] The early detection and timely treatment of cancers are very important for the patients to save their lives. Microorganism contamination in food is the main cause of foodborne diseases and 70% of patients with foodborne illnesses get sick after taking foods or water contaminated with pathogenic microorganisms. [4] Viruses are one of the major causes of infectious diseases and various public health events like the acquired immunodeficiency syndrome (AIDS), the severe acute respiratory syndrome (SARS), and the coronavirus disease 2019 (COVID-19). [5] Therefore, the timely and rapid detection of microorganisms is of great importance to ensure human health and public safety. [6] In addition, the detections of small molecules including mycotoxins and exogenous pollutions play an important role in food safety. Mycotoxins such as aflatoxin B1, ochratoxin A, and tenuazonic acid are classified as naturally occurring carcinogens by the World Health Organization that are extremely harmful to both humans and animals. [7] They not only directly threaten human life by contaminating food, but also indirectly affect human health through the food chain by contaminating feeds of animals. [8,9] Commonly used immunoassays include enzyme-linked immunosorbent assay (ELISA), quantum dot fluorescence immunoassay, fluorescence polarization immunoassay, colloidal gold immunoassay, chemiluminescent immunoassay (CLIA), electrochemical immunoassay, and microarray-based immunoassay. In spite of the different formats of immunoassays, they share the same principle that relies on the specific binding between antigenic determinants and antibody recognition sites. Therefore, it is of great significance to prepare specific antibodies with high affinity towards the target molecules to improve the specificity of immunoassays. Conventional immunoassays generally use polyclonal and monoclonal antibodies, but both types of antibodies have limitations such as poor specificity, low stability, and complicated preparation. To meet these challenges, a new type of antibody called "nanobody" has emerged. Nanobody is the heavy-chain-only antibody with the advantages of small size, high specificity, mass expression and high stability, which can act as a substitute for conventional antibodies in immunoassays and a promising low-cost immuno-affinity detection reagent.Immunoassay as a rapid and convenient method for detecting a variety of targets has attracted tremendous interest with its high specificity and sensitivity. Among the commonly used immunoassays, enzyme-linked immunosorbent assay has been widely used as a gold standard method in various fields that consists of two main components including a recognition element and an enzyme label. With the rapid advances in nanotechnology, nanobodies and nanozym...

show abstract

A fluorescence AuNPs-LISA: A new approach for Opisthorchis viverrini (Ov) antigen detection with a simple fluorescent enhancement strategy by surfactant micelle in urine samples

Cited by 10 publications

References 40 publications

Diagnosis of infectious diseases: complexity to convenience

Diagnosis of infectious diseases: complexity to convenience

Effects of day-to-day variation of Opisthorchis viverrini antigen in urine on the accuracy of diagnosing opisthorchiasis in Northeast Thailand

Nanobody and Nanozyme‐Enabled Immunoassays with Enhanced Specificity and Sensitivity

Contact Info

Product

Resources

About