A fluorescence enhancement probe based on BODIPY for the discrimination of cysteine from homocysteine and glutathione

Gong, Deyan; Tian, Yuejun; Yang, Chengduan; Iqbal, Anam; Wang, Zhiping; Liu, Weisheng; Qin, Wenwu; Zhu, Xiangtao; Guo, Huichen

doi:10.1016/j.bios.2016.05.013

Cited by 64 publications

(17 citation statements)

References 32 publications

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“…The resultant plot shows a linearity over the initial concentrations of L-Cys (Fig. This result suggests that our proposed sensor is better than that of the previous reports [9,18,19,25,28,[51][52][53][54][55][56][57][58][59][60][61][62][63]. The LOD of L-Cys was calculated to be 0.51 nM, revealing that this nanohybrid based sensor system showed a higher sensitivity towards the detection of L-Cys.…”

Section: Detection Of L-cysmentioning

confidence: 62%

Highly sensitive fluorogenic sensing of L-Cysteine in live cells using gelatin-stabilized gold nanoparticles decorated graphene nanosheets

Thirumalraj

Dhenadhayalan

Liu

et al. 2018

Sensors and Actuators B: Chemical

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a b s t r a c tA highly sensitive and selective fluorogenic sensing of L-Cysteine (L-Cys) was implemented based on gelatin stabilized gold nanoparticles decorated reduced graphene oxide (rGO/Au) nanohybrid. The rGO/Au nanohybrid was prepared by the one-pot hydrothermal method and well characterized by different physiochemical techniques. The nanohybrid exhibits a weak fluorescence of rGO due to the energy transfer from the rGO to Au NPs. The rGO/Au nanohybrid shows enhanced fluorescence activity due to the restoration of quenched fluorescence of rGO/Au nanohybrid in presence of L-Cys. The rGO/Au nanohybrid exhibits much lower detection limit of 0.51 nM for L-Cys with higher selectivity. The fluorescence sensing mechanism arose from the fluorescence recovery due to the stronger interaction between Au NPs and L-Cys, and consequently, the energy transfer was prevented between rGO and Au NPs. The practicability of rGO/Au sensor was implemented by invitro bioimaging measurements in Colo-205 (colorectal adenocarcinoma) and MKN-45 (gastric carcinoma) cancer live cells with excellent biocompatibility.

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Section: Detection Of L-cysmentioning

confidence: 62%

Highly sensitive fluorogenic sensing of L-Cysteine in live cells using gelatin-stabilized gold nanoparticles decorated graphene nanosheets

Thirumalraj

Dhenadhayalan

Liu

et al. 2018

Sensors and Actuators B: Chemical

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“…探针 9 对 Cys 表现出极高的选择性, 甚至可以从结构较为相似的 Hcy 和 GSH 中区分检测出 Cys. 2017 年, Sok 等 [35] 报道了两种检测 GSH 的具有 α,β- 2013 年, Long 等 [36] 报道了检测生物硫醇的基于迈 2016 年, Gong 等 [43] 报道了一种基于 ICT 机制的荧 Figure 26 Schematic illustration of reaction of 36 with biothiols 2017 年, Wang 等 [52] 报道了一种监测生物硫醇的近红外荧光探针 37 (图 27).…”

Section: 基于迈克尔加成反应unclassified

Progress in Fluorescence Sensors for Detection of Thiols

Yan¹,

Wang²,

Zhang³

et al. 2019

Chin. J. Org. Chem.

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Sulfhydryl compounds, such as cysteine (Cys), homocysteine (Hcy), glutathione (GSH) and so on, play an important role in the normal human physiological processes. Molecular fluorescent probes have attracted much attention of scholar in the detection of sulfhydryl compounds, since it has the advantage of good selectivity, high sensitivity, good biocompatibility and real-time in situ monitoring. At present, the design of thiol-based fluorescent probes is mainly based on the strong nucleophilicity of sulfhydryl groups. In this paper, the synthesis of fluorescent probes for detecting sulfhydryl compounds, which was reported from 2013 to 2018, is reviewed based on the different mechanism of the reaction between fluorescence probe and sulfhydryl, including Michael addition, cyclization of aldehyde groups, sulfonamides or sulfonates of the lysis, natural chemical linkage and so on. The detection limit of the probe, response time, equivalence ratio were elaborated.

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“…Hence, the selective and sensitive detection of Cys levels in a variety of food products like milk is of great significance. Up to date, several analytical methods have been reported for the determination of Cys, such as the high performance liquid chromatography [5], fluorescence spectroscopy [6], flow injection [7], colorimetry [8], chemiluminescence [9], and electrochemistry analysis [10]. Among them, the electrochemical analysis is one of the most frequently used and attractive analysis methods because of the inherent advantages of simplicity, easy miniaturization, high sensitivity, rapidity, and lower experimental costs.…”

Section: Introductionmentioning

confidence: 99%

Coating silver metal-organic frameworks onto nitrogen-doped porous carbons for the electrochemical sensing of cysteine

Zhai

Chen

et al. 2020

Microchim Acta

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Nitrogen-doped porous carbons (N-PC) were coated for the first time with silver metal-organic frameworks (Ag-MOF) by the hydrothermal route. The resulted N-PC@Ag-MOF composites present high stability because of the strong interaction between N atoms of N-PC and Ag + ions of Ag-MOF. It was discovered that the electrodes modified with N-PC@Ag-MOF composites display much higher conductivity than the one modified with Ag-MOF. Especially, they provide stable and sharp electrochemical signals of solid-state AgBr at a low potential approaching zero (i.e., 0.02 V), which may aid to overcome the drawback of the traditional electroanalysis at high overpotentials with serious interferences from the samples background. More importantly, the yielded AgBr signals selectively decrease induced by cysteine (Cys) through the specific thiol-bromine replacement reactions that transfer AgBr into non-electroactive Ag-Cys. The proposed method facilitates the selective detection of Cys with two linear working ranges of 0.10 to 100 μM and 100 to 1300 μM, respectively. The N-PC@Ag-MOF-based sensors have been used for detection of spiked Cys in milk samples with good recovery efficiencies. The developed electroanalysis strategy for probing Cys through the specific thiol-bromine replacement has potential applications in the food analysis fields.

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A fluorescence enhancement probe based on BODIPY for the discrimination of cysteine from homocysteine and glutathione

Cited by 64 publications

References 32 publications

Highly sensitive fluorogenic sensing of L-Cysteine in live cells using gelatin-stabilized gold nanoparticles decorated graphene nanosheets

Highly sensitive fluorogenic sensing of L-Cysteine in live cells using gelatin-stabilized gold nanoparticles decorated graphene nanosheets

Progress in Fluorescence Sensors for Detection of Thiols

Coating silver metal-organic frameworks onto nitrogen-doped porous carbons for the electrochemical sensing of cysteine

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