A Fluorescence Inner-Filter Effect Based Sensing Platform for Turn-On Detection of Glutathione in Human Serum

Abstract

A novel turn-on fluorescence assay was developed for the rapid detection of glutathione (GSH) based on the inner-filter effect (IFE) and redox reaction. Molybdenum disulfide quantum dots (MoS2 QDs), which have stable fluorescent properties, were synthesized with hydrothermal method. Manganese dioxide nanosheets (MnO2 NSs) were prepared by exfoliating the bulk δ-MnO2 material in bovine serum albumin (BSA) aqueous solution. The morphology structures of the prepared nanoparticles were characterized by transmission electron microscope (TEM). Studies have shown that the fluorescence of MoS2 QDs could be quenched in the presence of MnO2 NSs as a result of the IFE, and is recovered after the addition of GSH to dissolve the MnO2 NSs. The fluorescence intensity showed a good linear relationship with the GSH concentration in the range 20–2500 μM, the limit of detection was 1.0 μM. The detection method was applied to the analysis of GSH in human serum samples. This simple, rapid, and cost-effective method has great potential in analyzing GSH and in disease diagnosis.

Deep-Learning-Assisted Discriminative Detection of Vitamin B12 and Vitamin B9 by Fluorescent MoSe2 Quantum Dots

“…Fluorescence quenching may happen due to a variety of interactions between VB 12 and MoSe 2 QDs, including collision, ground-state complexation, energy transfer, and inner filter effect (IFE). − UV–vis spectroscopy can be employed to check the formation of ground-state complexes between the nanoprobe and quencher. Figure S6a shows the absorption spectra of MoSe 2 QDs and VB 12 .…”

Chlorine-doped MoS2 quantum dots embedded in a molecularly imprinted polymer for highly selective and sensitive optosensing of quercetin

“…However, the fabrication of carbon dots and graphene QDs is relatively complex and tedious and they are challenging to purify. 15 Recently, MoS 2 QDs have attracted much attention in imaging, 16 clinical diagnostics, 17 detection of biological agents and drugs, 18–20 chemical gas sensors, 21 etc. MoS 2 QDs have unique fluorescence properties, 22 facile preparation, and even better photostability and biocompatibility than graphene and carbon QDs.…”

Carbon dot as fluorescence sensor for glutathione in human serum samples: a review

“…[21][22][23][24][25][26][27] Carbon dots have garnered significant attention in various fields over the last few years. [28][29][30][31] CDs represent a novel category of carbon nanomaterials endowed with fascinating optical and photophysical characteristics. [32][33][34][35] The chemical characteristics of CDs, especially in the domains of analyte sensing.…”