A fluorescence resonance energy transfer (FRET) based “Turn-On” nanofluorescence sensor using a nitrogen-doped carbon dot-hexagonal cobalt oxyhydroxide nanosheet architecture and application to α-glucosidase inhibitor screening

Li, Guoliang; Kong, Weiheng; Zhao, Mei; Lu, Shuaimin; Gong, Peiwei; Chen, Guang; Xia, Lian; Wang, Hua; You, Jinmao; Wu, Yongning

doi:10.1016/j.bios.2015.12.094

Cited by 115 publications

(33 citation statements)

References 24 publications

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“…The absorption band at 3441 cm –1 was derived from the stretching vibrations of O–H and N–H, and the peak at 1516 cm –1 was attributed to the in-plane deformation vibration of the O–H bond . The absorption bands at 2928 and 1467 cm –1 were attributed to unsaturated stretching vibration and bending vibration of C–H. , The absorption peaks at 1728 and 1635 cm –1 belonged to the CO bond stretching vibration in the amide group and the carboxyl group, respectively. , The bands at 1286 and 1075 cm –1 were related to the stretching vibrations of C–O and C–O–C bond, respectively . The peaks at 1128 and 1039 cm –1 were pertained to the stretching vibration of Si–O–Si .…”

Section: Resultsmentioning

confidence: 99%

Chiral Fluorescent Silicon Nanoparticles for Aminopropanol Enantiomer: Fluorescence Discrimination and Mechanism Identification

Han

Chen

et al. 2020

Anal. Chem.

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As an important chiral molecule for the preparation of levofloxacin, the optical purity of L-aminopropanol has a crucial effect on the pharmacology and pharmacodynamics of levofloxacin. Therefore, it is of great significance to discriminate D-aminopropanol and L-aminopropanol. In this paper, an effective aminopropanol enantiomer recognition method was established on the basis of the chiral fluorescent silicon nanoparticles (SiNPs) probe. The chiral fluorescent SiNPs were fabricated via a one-step aqueous solution synthesis strategy, which avoided multiple steps, pressurizing operation, and time-consuming postmodified procedures. Significantly, D-aminopropanol could significantly enhance the fluorescence of the chiral SiNPs, while L-aminopropanol could not affect the fluorescence of the chiral SiNPs. This could have occurred because of the stronger interaction between the chiral SiNPs and D-aminopropanol than that of L-aminopropanol. Thus, the rapid and selective recognition of the aminopropanol enantiomer was ideally realized. The mechanism of the chiral SiNPs recognizing aminopropanol was simulated by density functional theory quantum mechanical calculations. Interestingly, this was also proved by the separation of aminopropanol enantiomer using this chiral SiNPs-modified silica column in normal phase liquid chromatography. To the best of our knowledge, this is the first time that the chiral fluorescent SiNPs were synthesized and used to detect the aminopropanol enantiomer successfully. This work will inspire additional syntheses of chiral silicon nanomaterials and other nanomaterials with excellent properties and will enable application of chiral nanomaterials to other fields.

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Section: Resultsmentioning

confidence: 99%

Chiral Fluorescent Silicon Nanoparticles for Aminopropanol Enantiomer: Fluorescence Discrimination and Mechanism Identification

Han

Chen

et al. 2020

Anal. Chem.

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“…The hexagonal shaped-CoOOH nanosheets with a strong wide-band absorption from 350 to 700 nm (Figures S10–S14) were employed as a promising dual-functional carrier and quencher for constructing nanocomposites. − Through electrostatic force, CDs were self-assembled on the surface of CoOOH nanosheets to fabricate the CDs/CoOOH composite. Carefully observing, crystalline lattice fringes of CDs (0.21 nm) and CoOOH nanosheets (0.27 nm) simultaneously existed in the CDs/CoOOH composite (Figure A,B).…”

Section: Resultsmentioning

confidence: 99%

Smartphone-Assisted Robust Sensing Platform for On-Site Quantitation of 2,4-Dichlorophenoxyacetic Acid Using Red Emissive Carbon Dots

Han

Yan

et al. 2020

Anal. Chem.

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On-site quantitative analysis of pesticide is of significant importance for addressing serious public health issues in clinical, food, and environmental settings. Herein, we designed a novel smartphone-assisted sensing platform for on-site monitoring of 2,4-dichlorophenoxyacetic acid (2,4-D) based on carbon dots/cobalt oxyhydroxide nanosheet (CDs/CoOOH) composite. In this work, a red emissive CDs/CoOOH composite was proposed as a signal indicator for shielding background interference, enhancing anti-interference capability. 2,4-D as an inhibitor of alkaline phosphatase could specifically suppress the production of ascorbic acid, which restrained in situ etching of the CDs/CoOOH composite and further triggered the fluorescence response of the biosensor. By employing a lab-on-smartphone based device and self-designed application software, the fluorescence image was directly captured and analyzed with a sensitive detection limit of 100 μg L–1 for 2,4-D. Merging the CDs/CoOOH composite-based fluorometric system with the smartphone-assisted optical reader, such a cost-effective and portable platform provided a new sight for on-site monitoring of pesticide and expanded application prospect in the field of biological analysis.

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“…Traditional analytical methods for detecting α-glucosidase activity include chromatographic [ 11 , 12 ], fluorescence [ 13 , 14 ], and colorimetric [ 15 , 16 ] methods, which usually suffer from the disadvantages of time and reagent consuming, requiring professional operators and expensive instruments. Although they are commonly applied in many studies, they are not feasible for point-of-care testing (POCT) and hinder their general application in resource restrained areas.…”

Section: Introductionmentioning

confidence: 99%

Personal Glucose Meter for α-Glucosidase Inhibitor Screening Based on the Hydrolysis of Maltose

et al. 2021

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As a key enzyme regulating postprandial blood glucose, α-Glucosidase is considered to be an effective target for the treatment of diabetes mellitus. In this study, a simple, rapid, and effective method for enzyme inhibitors screening assay was established based on α-glucosidase catalyzes reactions in a personal glucose meter (PGM). α-glucosidase catalyzes the hydrolysis of maltose to produce glucose, which triggers the reduction of ferricyanide (K3[Fe(CN)6]) to ferrocyanide (K4[Fe(CN)6]) and generates the PGM detectable signals. When the α-glucosidase inhibitor (such as acarbose) is added, the yield of glucose and the readout of PGM decreased accordingly. This method can achieve the direct determination of α-glucosidase activity by the PGM as simple as the blood glucose tests. Under the optimal experimental conditions, the developed method was applied to evaluate the inhibitory activity of thirty-four small-molecule compounds and eighteen medicinal plants extracts on α-glucosidase. The results exhibit that lithospermic acid (52.5 ± 3.0%) and protocatechualdehyde (36.8 ± 2.8%) have higher inhibitory activity than that of positive control acarbose (31.5 ± 2.5%) at the same final concentration of 5.0 mM. Besides, the lemon extract has a good inhibitory effect on α-glucosidase with a percentage of inhibition of 43.3 ± 3.5%. Finally, the binding sites and modes of four active small-molecule compounds to α-glucosidase were investigated by molecular docking analysis. These results indicate that the PGM method is feasible to screening inhibitors from natural products with simple and rapid operations.

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A fluorescence resonance energy transfer (FRET) based “Turn-On” nanofluorescence sensor using a nitrogen-doped carbon dot-hexagonal cobalt oxyhydroxide nanosheet architecture and application to α-glucosidase inhibitor screening

Cited by 115 publications

References 24 publications

Chiral Fluorescent Silicon Nanoparticles for Aminopropanol Enantiomer: Fluorescence Discrimination and Mechanism Identification

Chiral Fluorescent Silicon Nanoparticles for Aminopropanol Enantiomer: Fluorescence Discrimination and Mechanism Identification

Smartphone-Assisted Robust Sensing Platform for On-Site Quantitation of 2,4-Dichlorophenoxyacetic Acid Using Red Emissive Carbon Dots

Personal Glucose Meter for α-Glucosidase Inhibitor Screening Based on the Hydrolysis of Maltose

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