A fluorescence study of apolipoprotein localization in relation to lipids in serum low density lipoproteins

Dobretsov, G. E.; Spirin, M. M.; Chekrygin, O.V.; Karmansky, Israel; Dmitriev, V. M.; Vladimirov, Yu. A.

doi:10.1016/0005-2760(82)90147-3

Cited by 38 publications

(9 citation statements)

References 24 publications

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“…The R 0 value for the tryptophan-Py-3-Py donor-acceptor pair was 26.9 Å, which was comparable to the values for tryptophan-pyrene pair (Dobretsov et al, 1982;Tahara et al, 1992). In the calculation of R 0 , the value of k 2 was 2/3, and that of the (Schroeder et al, 1988;Sweet et al, 1987).…”

Section: Effects Of E2 On the Bulk And The Annular Lateral Diffusion supporting

confidence: 51%

Selective Fluidization of Synaptosomal Plasma Membrane Vesicles by 17β-Estradiol

Lee

Park

Jang

et al. 2017

BSL

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Estrogens are effective neuroprotectants in vivo and in vitro. To obtain a better insight into the molecular mechanisms of action of neuroprotection by 17β-estradiol (E2), we examined the differential effects of E2 on the fluidity of synaptosomal plasma membrane vesicles (SPMV) isolated from rat cerebral cortex. Intramolecular excimerization of 1,3-di(1-pyrenyl)-propane (Py-3-Py) was used to investigate the effects of E2 on the bulk and annular lateral diffusion of the SPMV. In addition, we examined the effects of E2 on the rotational diffusion of individual leaflet of SPMV exploiting selective quenching of outer monolayer 1,6-diphenyl-1,3,5-hexatriene (DPH) fluorescence by trinitrophenyl groups. The Förster distance R 0 value for the tryptophan-Py-3-Py donor-acceptor pair was 26.9 Å. E2 increased the lateral mobility of both bulk and annular lipids in SPMV in a dose-dependent manner, but a larger effect on bulk lipids was observed. Although E2 decreased the anisotropy of DPH in SPMV, E2 had a greater fluidizing effect on the outer leaflet compared to the inner leaflet. These results suggest that E2 selectively fluidizes the more fluid regions within SPMV. It is highly probable that E2 mostly fluidizes the bulk lipids, away from either annular lipids or lipid rafts, in the outer leaflet of SPMV. This selective fluidization may be one of the nongenomic mechanisms of neuroprotection by E2.

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Section: Effects Of E2 On the Bulk And The Annular Lateral Diffusion supporting

confidence: 51%

Selective Fluidization of Synaptosomal Plasma Membrane Vesicles by 17β-Estradiol

Lee

Park

Jang

et al. 2017

BSL

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“…Considering that the Förster radius (the RET-limiting distance) of the ANS-Py-3-Py donor-acceptor pair is 3 nm (Bae et al, 2005;Dobretsov et al, 1982;Jang et al, 2004a, b), Py-3-Py was excited at 330 nm, and the monomer fluorescence intensity of Py-3-Py was read at 379 nm. 30 µM ANS (60 µL of 1 mm solution in water) was then added.…”

Section: Determination Of Thickness Of Spmv Lipid Bilayermentioning

confidence: 99%

Effects of dimyristoylphosphatidylethanol and ethanol on thickness of neuronal membrane lipid bilayers

et al. 2009

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The aim of this study was to provide a basis for examining the molecular mechanism for the pharmacological action of ethanol. Energy transfer between the surface fluorescent probe 1-anilinonaphthalene-8-sulfonic acid and hydrophobic fluorescent probe 1,3-di(1-pyrenyl)propane was used to examine the effect of both dimyristoylphosphatidylethanol (DMPEt) and ethanol on the thickness (D) of the synaptosomal plasma membrane vesicles (SPMV) isolated from the bovine cerebral cortex. The thickness (D) of the intact SPMV was 1.044 +/- 0.008 (arbitrary units, n=5) at 37 degrees C (pH 7.4). Both DMPEt and ethanol decreased the thickness of the SPMV lipid bilayer in a dose-dependent manner with a significant decrease in thickness observed at 5 microM and 25 mM, respectively. It was assumed that both ethanol and DMPEt cause interdigitation in the SPMV lipid bilayers. The effects of ethanol on the neuronal membranes were attributed to its direct and indirect actions. The indirect action of ethanol refers to the action of phosphatidylethanol, which is an ethanol abnormal metabolite, on the neuronal membranes. The decrease in membrane thickness by both DMPEt and ethanol might be responsible for some, but not all of its anesthetic actions.

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“…Taking into account that the Förster radius (the RET-limiting distance) for the tryptophan-Py-3-Py donor-acceptor pair is 3 nm (Dobretsov et al, 1982;Jang et al, 2004a,b;Bae et al, 2005), only Py-3-Py located in annular lipids (close to proteins) was excited, and the fluidity of annular lipids was considered proportional to I /I (Yun et al, 1993(Yun et al, , 1994Kang et al, 1996;Jang et al, 2004a,b;Bae et al, 2005).…”

Section: Determination Of Annular Lipid Fluidity In Spmvsmentioning

confidence: 99%

The effect of propoxycaine·HCl on the physical properties of neuronal membranes

Lee¹,

Kim²,

Mun³

et al. 2008

Chemistry and Physics of Lipids

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A fluorescence study of apolipoprotein localization in relation to lipids in serum low density lipoproteins

Cited by 38 publications

References 24 publications

Selective Fluidization of Synaptosomal Plasma Membrane Vesicles by 17β-Estradiol

Selective Fluidization of Synaptosomal Plasma Membrane Vesicles by 17β-Estradiol

Effects of dimyristoylphosphatidylethanol and ethanol on thickness of neuronal membrane lipid bilayers

The effect of propoxycaine·HCl on the physical properties of neuronal membranes

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