A Fluorescence Study of the Interaction of Anticancer Drug Molecule Doxorubicin Hydrochloride in Pluronic P123 and F127 Micelles

Abstract

Drug delivery systems for the sustained and target delivery of doxorubicin to tumor cells are a topic of interest due to the efficacy of the doxorubicin in cancer treatment. The use of polymers such as Pluronic is being studied widely for the formulation of doxorubicin hydrochloride. However, the basic understanding of the physicochemical properties of pluronic micelles in presence of doxorubicin hydrochloride is a very essential topic of study. Doxorubicin hydrochloride is fluorescent; this helped us to study its sensitivity towards the Pluronic microenvironment using the fluorescence technique. In this work, the interaction and place of location of doxorubicin hydrochloride in Pluronic F127 and P123 micelles has been studied extensively using steady-state fluorescence intensity, dynamic fluorescence lifetime, quenching studies, dynamic light scattering, and zeta potential measurements, at different Pluronic concentrations. Using a fluorescence quenching experiment, doxorubicin hydrochloride was found to reside near the hydrophilic PEO corona region of the Pluronic micelles. For both the Pluronic, in the concentration range of study, the micellar size was found to be below 30 nm; this may have a greater advantage for various applications.

Modification of Octenyl Succinic Anhydride Starch by Grafting Folic Acid and its Potential as an Oral Colonic Delivery Carrier

“…DOX exhibits natural fluorescence properties owing to the benzene ring in its structure. [ 28 ] The decay of the fluorescence intensity of DOX reflects the interaction of the benzene ring of DOX with other groups. Figure shows that the fluorescence intensity of the DOX solution diminished after combining with OSA starch, indicating that hydrophobic interactions may exist between the long‐chain alkanes of the OSA groups and the benzene rings of DOX.…”

Functionalized Mesoporous Silica as Doxorubicin Carriers and Cytotoxicity Boosters

“…The standard Gibbs free energy was determined according to Equation (8): ΔG 0 binding = −2.303 RT log K b where R is the universal gas constant (8.3145 J mol −1 K −1 ); T is temperature (Kelvin); and K b is the association constant from the Benesi–Hildebrand Equation (7). The calculated value of ΔG 0 binding is −28.48 kJ mol −1 (−6.807 kcal mol −1 ) for Dox: AG and −27.16 kJ mol −1 (−6.49 kcal mol −1 ) for Dox: R-SH, characteristic of H-bonding interactions [ 48 ]. This result shows that, in aqueous dilute solutions, both functional groups are able to bond to Dox with similar strength.…”

Rational Understanding of Loading and Release of Doxorubicin by UV-Light- and pH-Responsive Poly(NIPAM-co-SPMA) Micelle-like Aggregates

“…In those systems, it is well-accepted that poorly water-soluble active principles interact with the hydrophobic molecular segments at the inner part of the micelle-like aggregates, creating a hollow drug container. Moreover, different types of interactions could be expected according to the chemical nature of the polymeric components and the model drugs, such as hydrogen bonding, ionic, hydrophobic, π–π stacking, electrostatic, and stereocomplex interactions. − However, very scarce studies have been focused on studying these interactions. Notably, dissipative particle dynamics simulations were applied to model the interactions of doxorubicin with polycaprolactone-β-polyethylene glycol methyl ether methacrylate and poly­(ethylene glycol) methyl ether-β-poly­( N , N -diethylamino ethyl methacrylate) micelles showing hydrophobic interactions in micelle core .…”

“…Notably, dissipative particle dynamics simulations were applied to model the interactions of doxorubicin with polycaprolactone-β-polyethylene glycol methyl ether methacrylate and poly­(ethylene glycol) methyl ether-β-poly­( N , N -diethylamino ethyl methacrylate) micelles showing hydrophobic interactions in micelle core . In another work, Dox-HCl was found to electrostatically interact with the hydrophilic poly­(ethylene oxide) units at the corona region of pluronic micelles, thus affecting their size and photoluminescent response . More studies in this field are highly demanded to better understand the behavior of this system.…”

“…20 In another work, Dox-HCl was found to electrostatically interact with the hydrophilic poly(ethylene oxide) units at the corona region of pluronic micelles, thus affecting their size and photoluminescent response. 21 More studies in this field are highly demanded to better understand the behavior of this system.…”