A fluorescence turn-on H2O2 probe exhibits lysosome-localized fluorescence signals

Song, Dayoung; Lim, Joo Han; Cho, Somin; Park, Su Jin; Cho, Jaeheung; Kang, Dongmin; Rhee, Sue Goo; You, Youngmin; Nam, Wonwoo

doi:10.1039/c2cc31632c

Cited by 74 publications

(39 citation statements)

References 24 publications

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“…We highlight two recent examples 77,78 of non-haem iron-peroxide reactivity for chemoselective monitoring of H 2 O 2 , inspired by the haem-mediated horseradish peroxidase–Amplex red ( N -acetyl-3,7-dihydroxyphenoxazine) assay that has been used extensively to indirectly quantify the activity of various H 2 O 2 -producing enzymes. Hitomi and co-workers recently developed the H 2 O 2 probe MBFh1 by linking an Fe( III )-polypyridine complex to a non-fluorescent 3,7-dihydroxyphenoxazine amide (Fig.…”

Section: Metal-mediated Reaction-based Fluorescent Probesmentioning

confidence: 99%

“…Reaction between H 2 O 2 and MBFh1 leads to iron-mediated oxidation, cleavage and release of a fluorescent resorufin dye. Nam’s laboratory has elegantly exploited a classic zinc-sensor motif for metal-based H 2 O 2 detection 78 . Formation of the bis-iron complex of Zinpyr-1 (ZP1) turns-off its basal fluorescence.…”

Section: Metal-mediated Reaction-based Fluorescent Probesmentioning

confidence: 99%

“…Addition of H 2 O 2 triggers iron-induced oxidative cleavage of the dipicolylamine ligands on the ZP1 scaffold to produce the corresponding bis-carboxylate fluorescein with concomitant release of iron ions (Fig. 5f) 78 . This probe shows selectivity for H 2 O 2 over many ROS and can be used to track changes in H 2 O 2 localized to lysosomes in living cells.…”

Section: Metal-mediated Reaction-based Fluorescent Probesmentioning

confidence: 99%

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Reaction-based small-molecule fluorescent probes for chemoselective bioimaging

2012

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The dynamic chemical diversity of elements, ions and molecules that form the basis of life offers both a challenge and an opportunity for study. Small-molecule fluorescent probes can make use of selective, bioorthogonal chemistries to report on specific analytes in cells and in more complex biological specimens. These probes offer powerful reagents to interrogate the physiology and pathology of reactive chemical species in their native environments with minimal perturbation to living systems. This Review presents a survey of tools and tactics for using such probes to detect biologically important chemical analytes. We highlight design criteria for effective chemical tools for use in biological applications as well as gaps for future exploration.

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Section: Metal-mediated Reaction-based Fluorescent Probesmentioning

confidence: 99%

Section: Metal-mediated Reaction-based Fluorescent Probesmentioning

confidence: 99%

Section: Metal-mediated Reaction-based Fluorescent Probesmentioning

confidence: 99%

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Reaction-based small-molecule fluorescent probes for chemoselective bioimaging

2012

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“…Another fluorescein-based probe relying on the release of a quencher ionophore was published by Song et al [54]. The iron center is activated by H2O2 with subsequent N-dealkylation of the ligand, Another type of cleaving group is sulfonate-based ( Figure 2; (3-5)).…”

Section: Oxidative Cleavage-based Probesmentioning

confidence: 99%

“…When it comes to choice of the oxidative cleavage groups (OCGs), there are also many possibilities and many different detection schemes have been proposed in the literature [49,54,55]. Here we focus only on a few examples, but note that the points listed below also apply to most other When it comes to choice of the oxidative cleavage groups (OCGs), there are also many possibilities and many different detection schemes have been proposed in the literature [49,54,55]. Here we focus only on a few examples, but note that the points listed below also apply to most other OCGs (Figure 2).…”

Section: Oxidative Cleavage-based Probesmentioning

confidence: 99%

Possibilities and Challenges for Quantitative Optical Sensing of Hydrogen Peroxide

2017

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Abstract:Hydrogen peroxide (H 2 O 2 ) plays a key role in many biological processes spanning from coral bleaching, over cell signaling to aging. However, exact quantitative assessments of concentrations and dynamics of H 2 O 2 remain challenging due to methodological limitationsespecially at very low (sub µM) concentrations. Most published optical detection schemes for H 2 O 2 suffer from irreversibility, cross sensitivity to other analytes such as other reactive oxygen species (ROS) or pH, instability, temperature dependency or limitation to a specific medium. We review optical detection schemes for H 2 O 2 , compare their specific advantages and disadvantages, and discuss current challenges and new approaches for quantitative optical H 2 O 2 detection, with a special focus on luminescence-based measurements. We also review published concentration ranges for H 2 O 2 in natural habitats, and physiological concentrations in different biological samples to provide guidelines for future experiments and sensor development in biomedical and environmental science.

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Detection of Metal Ions, Anions and Small Molecules Using Metal Complexes

Wang

Franz

2014

Inorganic Chemical Biology

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Biology is a complex network of molecules that interact, organize, and communicate. We cannot readily visualize most of these species, and yet we are interested in tracking their presence within cells, tissues, or biological fluids in order to provide a deeper understanding of their physiological significance. There has been considerable effort, therefore, to develop chemical sensors that can "see" specific analytes in biological systems.A chemical sensor reports the presence of an analyte by converting a recognition event (usually, but not always, a binding event) into a spectroscopic signal. The analytes of interest include a broad swath of molecule types, including anions, reactive small molecules, proteins, nucleic acids, metal ions, or any other biological or foreign molecule. The spectroscopic signals can be colorimetric, luminescent, magnetic, electrochemical, or radioactive. Currently used sensors largely rely on organic dyes, fluorescent proteins, nanoparticles, and metal complexes for responsive signal transduction. In this chapter, we focus exclusively on the use of metal complexes as design features for chemical sensors. Subsets of this category include radiometals and electroactive metals, which are not covered further here. Instead, we direct interested readers to a recent comprehensive review of the coordination chemistry of radiometals and their application as probes in PET and SPECT imaging [1], as well as reviews on the use of electroactive transition metal receptors for electrochemical sensing applications [2,3].

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A fluorescence turn-on H2O2 probe exhibits lysosome-localized fluorescence signals

Abstract: A new fluorescence turn-on probe that responds exclusively to H(2)O(2) exhibits subcellular localized fluorescence staining of lysosomes.

Cited by 74 publications

References 24 publications

Reaction-based small-molecule fluorescent probes for chemoselective bioimaging

Reaction-based small-molecule fluorescent probes for chemoselective bioimaging

Possibilities and Challenges for Quantitative Optical Sensing of Hydrogen Peroxide

Detection of Metal Ions, Anions and Small Molecules Using Metal Complexes

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