A fluorescent antibody technique for the detection of the group-specific antigen of feline leukemia virus in infected tissue-culture cells

Immunofluorescent detection of murine and hamster C‐type virus species‐specific (gs‐1) determinants by monospecific guinea‐pig sera and interspecies‐specific (gs‐3) determinants by tumor‐bearing rat sera

“…The results reported here demonstrate the efficacy of the direct FA test and monospecific guinea-pig antisera for detecting species-specific (gs-l) mouse and hamster C-type virus antigenic determinants. In agreement with the findings of others (Chuat et al, 1971;Ubertini et al, 1971;Kelloff and Vogt, 1966;Fleissner, 1970), FA staining was limited to the cytoplasm of infected cells with no evidence of intranuclear virion antigen localization. Two types of cytoplasmic staining were observed.…”

“…This raises questions concerning the sensitivity of FA for detecting gs-I antigens, especially in systems where the infection has been well established. It is of interest, in this respect, that Ubertini et al (1971) reported that FA was more sensitive than agar gel immunodiffusion or electron microscopy for detecting feline C-type antigens during early cell passages following infection with feline leukemia virus, but this differential sensitivity disappeared during later cell passages.…”

“…The detection of gs-l antigens by immunofluorescence (FA) using antisera from heterologous tumor-bearing animals or absorbed sera Received: July 5, 1971. from animals immunized with disrupted virus has been reported (Lejneva and Abelev, 1970;Ubertini et al, 1971). Methods are now available, however, for purification of gs antigens utilizing electrofocus techniques (Gregoriades and Old, 1969), and monospecific antisera to gs-1 antigens of feline, murine and hamster C-type viruses have been prepared (Oroszlan et al, 1971b;Kelloff et al, 1970c;Oroszlan et al, 1971a).…”

Immunofluorescent Studies of RD-114 Virus Replication in Cell Culture

“…Other reports described the use of immunofluorescence technique to study the gs antigen of murine (Lejneva & Abelev, 197o;Chuat et al 1971) and feline (Ubertini et al 1971) leukaemia viruses. Hampar and co-workers (Hampar et al I97I) also used this technique to study the gs-I antigen of both rnurine and hamster type-C viruses using antisera to gs-I antigen which had been purified by electrofocusing (Gregoriades & Old, 1969).…”

Comprehensive Virology

“…Less-sensitive methods utilize (1) homologous interference, where cells infected with a nontransforming virus are refractory to infection with transforming virus from the same subgroup (Rubin, 1960;Vogt and Ishizaki, 1966), (2) complement fixation, in which infected cells synthesize sufficient viral antigen to be detected by complement fixation (Huebner et al, 1964;Hartley et al, 1965), or (3) fluorescent antibody to viral antigens (Vogt and Rubin, 1962;Ubertini et al, 1971). These methods are most often used in the detection of generalized virus infection, or have specific application in experiments.…”