A fluorescent aptasensor based on nitrogen-doped carbon supported palladium and exonuclease III-assisted signal amplification for sensitive detection of AFB1

Microelectromechanical Microsystems-Supported Photothermal Immunoassay for Point-of-Care Testing of Aflatoxin B1 in Foodstuff

“…Additional evaluation of the two methods was likewise performed using the classical Student t test, and t exp values were found to be less than 2.78 for all test results (Table S1). Last but not the least, a side-by-side comparison of the detection range of the sensor with previous reports found that the sensor had a wide dynamic response range with practicality compared to other efforts. ,− In addition, the sensor was expected to be of maximum utility in POC testing thanks to its freedom from expensive sensing device support and the involvement of well-educated testers.…”

Multiple Isothermal Amplification Coupled with CRISPR–Cas14a for the Naked-eye and Colorimetric Detection of Aflatoxin B1

“…3,4 Due to AFB1's extreme toxicity and excellent thermal stability, it contaminates a wide range of food and agricultural products, leading to significant losses in human productivity and adverse effects on public health. 5,6 The United States Food and Drug Administration has set the allowable detectable amount of AFB1 at 20 ppb (μg mL −1 ), and the European Union has established a maximum allowable detection limit of 6.4 nM for AFB1 in cereals and their products. 7,8 Therefore, there is an urgent need to develop a rapid, simple, and sensitive method for accurately quantifying AFB1 and warning against food contamination.…”

“…It can induce various diseases, including cancer, acute hepatitis, hemorrhagic necrosis, hepatocellular steatosis, and bile duct hyperplasia. , AFT is a derivative of furocoumarin, a secondary metabolite produced by a variety of filamentous fungi such as Aspergillus flavus and Aspergillus parasiticus. Over 10 types, including B1, B2, G1, G2, M1, and M2, have been isolated and identified. , Due to AFB1’s extreme toxicity and excellent thermal stability, it contaminates a wide range of food and agricultural products, leading to significant losses in human productivity and adverse effects on public health. , The United States Food and Drug Administration has set the allowable detectable amount of AFB1 at 20 ppb (μg mL –1 ), and the European Union has established a maximum allowable detection limit of 6.4 nM for AFB1 in cereals and their products. , Therefore, there is an urgent need to develop a rapid, simple, and sensitive method for accurately quantifying AFB1 and warning against food contamination.…”