A fluorescent probe capable of detecting H2S at submicromolar concentrations in cells

Abstract

A new internal charge transfer (ICT) fluorescent probe for highly selective and sensitive monitoring of H(2)S has been developed. The design takes advantage of the facile reduction of non-fluorescent hydroxyamine naphthalimide by H(2)S to highly fluorescent amine naphthalimide. It has been demonstrated that the probe is able to detect H(2)S at submicromolar concentrations in cells.

Fluorescent probes for hydrogen sulfide detection and bioimaging

“…41 During the reduction of nitro groups to amines, hydroxylamine derivatives are produced as intermediates. The hydroxylamine moiety can be reduced more easily than the nitro group.…”

A Copper Porphyrin for Sensing H2S in Aqueous Solution via a “Coordinative‐Based” Approach

“…Existing fluorescencebased probes implemented so far have been subdivided into four different categories (depending on the reaction mechanism by which analyte recognition occurs): [1,19] (i) azide-toamine reduction; [12,[21][22][23][24][25][26][27] (ii) nucleophilic addition; [28][29][30][31] (iii) copper displacement; [32][33][34][35][36] and (iv) nitro-to-amine reduction. [37,38] All the sensors belonging to the above categories make use of organic molecules that change their fluorescence intensity when interacting with H 2 S. Sensors belonging to category (iii) constitute an exception since in this case the molecule acting as the recognition element is a metal complex. Here, recognition builds on the displacement of the metal from the fluorophore's environment to oxidants such as H 2 O 2 .…”

Metal oxides and metal salt nanostructures for hydrogen sulfide sensing: mechanism and sensing performance

“…In the past, many quantum dot, metal nanoparticles and organic dyes have been utilized in the gas detection including the reducing gas H 2 S. [28][29][30][31][32][33][34][35][36] These sensors are mostly based on the optical properties of the materials as a sensing signal. The optical signal undoubted provides sensitive and naked eye approach to the gas recognition.…”