A fluorescent probe for H₂S in vivo with fast response and high sensitivity

Abstract

In this work, we design and synthesize a new near-infrared (NIR) ratiometric fluorescent probe FD-H2S for the highly sensitive (DL 68.2 nM) detection of H2S with fast response (15 s), large emission shift (220 nm) and excellent enhancement (168-fold in ratiometric value). The probe could be applied for monitoring and imaging of exogenous or endogenous H2S in live MCF-7 cells and in live mice with the fastest response.

A review: Red/near‐infrared (NIR) fluorescent probes based on nucleophilic reactions of H2S since 2015

“…NaHS]. [ 101 ] Statistics was used to quantify the relative concentration of basal, exogenous, and endogenous H 2 S in living MCF‐7 cells. A commercially available, mitochondria‐localizing dye (Mito Tracker Green FM) was employed for a co‐localization study in MCF‐7 cells.…”

A turn-on fluorescent probe with a dansyl fluorophore for hydrogen sulfide sensing

“…19 Compared to these methods, uorescence analysis has proven to be a promising option because of its high sensitivity, high temporal and spatial detection resolutions, and the ability for conducting in situ monitoring of reactive and transient target analytes. [20][21][22][23][24][25][26][27] Generally, the uorescence probes for H 2 S mainly based on reduction reactions, [28][29][30][31][32] nucleophilic addition reactions, [33][34][35] and thiolysis reactions. [36][37][38] In addition, metal displacement approach utilizes the strong affinity of metal ions with suldes ions to rapidly attain reaction equilibrium, and achieve the realtime detection of H 2 S. 39,40 Based on metal displacement approach of copper metal complexes, some colorimetric or uorescent sensors for H 2 S have been emerging in recent years, owning to the low solubility product of CuS (K sp ¼ 6.3 Â 10 À36 ).…”

Using calculations of the electronically excited states for investigation of fluorescent sensors: A review

“…Fluorescent sensors are based on the transforms of fluorescence signal resulting from the interaction of the analytes with sensors. During the sensing process, there is a change in intensity from weak or non-fluorescence to strong fluorescence, [1][2][3] or vice versa; [4][5][6] or a shift in maximum fluorescence wavelength. [7][8][9] Fluorescent sensor is called a chemosensor when its reaction with a specific analyte is reversible.…”