A fluorogenic substrate for the detection of lipid amidases in intact cells

Casasampere, Mireia; Ung, Johnson; Iñáñez, Alejandro; Dufau, Carine; Tsuboi, Kazuhito; Casas, Josefina; Tan, Su-Fern; Feith, David J.; Andrieu-Abadie, Nathalie; Segui, Bruno; Loughran, Thomas P.; Abad, José Luis; Fabrias, Gemma

doi:10.1016/j.jlr.2024.100520

Journal of Lipid Research

2024

DOI: 10.1016/j.jlr.2024.100520

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A fluorogenic substrate for the detection of lipid amidases in intact cells

Mireia Casasampere,

Johnson Ung,

Alejandro Iñáñez

et al.

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Fluorescence-Based Enzyme Activity Assay: Ascertaining the Activity and Inhibition of Endocannabinoid Hydrolytic Enzymes

Ciuffreda,

Xynomilakis,

Casati

et al. 2024

IJMS

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The endocannabinoid system, known for its regulatory role in various physiological processes, relies on the activities of several hydrolytic enzymes, such as fatty acid amide hydrolase (FAAH), N-acylethanolamine-hydrolyzing acid amidase (NAAA), monoacylglycerol lipase (MAGL), and α/β-hydrolase domains 6 (ABHD6) and 12 (ABHD12), to maintain homeostasis. Accurate measurement of these enzymes’ activities is crucial for understanding their function and for the development of potential therapeutic agents. Fluorometric assays, which offer high sensitivity, specificity, and real-time monitoring capabilities, have become essential tools in enzymatic studies. This review provides a comprehensive overview of the principles behind these assays, the various substrates and fluorophores used, and advances in assay techniques used not only for the determination of the kinetic mechanisms of enzyme reactions but also for setting up kinetic assays for the high-throughput screening of each critical enzyme involved in endocannabinoid degradation. Through this comprehensive review, we aim to highlight the strengths and limitations of current fluorometric assays and suggest future directions for improving the measurement of enzyme activity in the endocannabinoid system.

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Fluorescence-Based Enzyme Activity Assay: Ascertaining the Activity and Inhibition of Endocannabinoid Hydrolytic Enzymes

Ciuffreda,

Xynomilakis,

Casati

et al. 2024

IJMS

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show abstract

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A fluorogenic substrate for the detection of lipid amidases in intact cells

Cited by 1 publication

References 53 publications

Fluorescence-Based Enzyme Activity Assay: Ascertaining the Activity and Inhibition of Endocannabinoid Hydrolytic Enzymes

Fluorescence-Based Enzyme Activity Assay: Ascertaining the Activity and Inhibition of Endocannabinoid Hydrolytic Enzymes

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