A fluorometric assay of peroxidase activity utilizing 2′,7′-dichlorofluorescein with thiocyanate: application to the study of salivary secretion

Proctor, Gordon; Chan, Ka-Ming

doi:10.1016/0165-022x(94)90065-5

Cited by 33 publications

(28 citation statements)

References 19 publications

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“…For determination of salivary amylase activity, 10 l of saliva was diluted 1: 100 in MES buffer Peroxidase. Peroxidase activity was determined as described in detail previously [Proctor and Chan, 1994;Hannig et al, 2008b]. In the presence of peroxidase and hydrogen peroxide, fluorogenic 2 ,7 -dichlorofluorescein (LDCF) is converted to fluorescent dichlorofluorescein (DCF).…”

Section: Procedure Evaluation Criteria and Responsibilitiesmentioning

confidence: 99%

Enzyme Activities in the Oral Fluids of Patients Suffering from Bulimia: A Controlled Clinical Trial

et al. 2012

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Patients with bulimia nervosa are at high risk for dental erosion. However, not all bulimic patients suffer from erosion, irrespective of the severity of their eating disorder. It is often speculated that differences in the saliva are important, however, little is known about salivary parameters in bulimic patients, particularly directly after vomiting. The aim of the clinical trial was to compare different salivary parameters of subjects suffering from bulimia with those of healthy controls. Twenty-eight subjects participated (14 patients with bulimia nervosa, 7 of them with erosion; 14 matched healthy controls). Resting and stimulated saliva of all participants was analysed as well as saliva collected from bulimic patients directly and 30 min after vomiting. Parameters under investigation were flow rate, pH, buffering capacity and the enzyme activities of proteases in general, collagenase, pepsin, trypsin, amylase, peroxidase, and lysozyme. Regarding flow rate, pH and buffering capacity only small differences were found between groups; buffering capacity directly after vomiting was significantly lower in bulimic subjects with erosion than in subjects without erosion. Differences in enzymatic activities were more pronounced. Activities of proteases, collagenase and pepsin in resting and proteases in stimulated saliva were significantly higher in bulimic participants with erosion than in controls. Peroxidase activity was significantly decreased by regular vomiting. Proteolytic enzymes seem to be relevant for the initiation and progression of dental erosion directly after vomiting, maybe by both hydrolysis of demineralized dentine structures as well as modulation of the pellicle layer.

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Section: Procedure Evaluation Criteria and Responsibilitiesmentioning

confidence: 99%

Enzyme Activities in the Oral Fluids of Patients Suffering from Bulimia: A Controlled Clinical Trial

et al. 2012

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“…Samples were diluted with water and a human albumin-globulin mixture (Sigma, Poole, UK) was used to produce a standard curve. Peroxidase activity was measured as described by Proctor & Chan (1994). One hundred microlitres of stock diacetyldichlorofluorescin (5 ² 10¦Ç Ò in ethanol) was activated by adding 900 ìl of 0.01 Ò NaOH, left at room temperature for 30 min and then diluted with 67 mÒ phosphate buffer (pH 6.0).…”

Section: Biochemical Analyses Of Salivary Compositionmentioning

confidence: 99%

Immunoglobulin A secretion into saliva during dual sympathetic and parasympathetic nerve stimulation of rat submandibular glands

Carpenter¹,

Proctor²,

Anderson

et al. 2000

Exp. Physiol.

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“…Peroxidase activity was measured as described by Proctor & Chan (1994). One hundred microlitres of a stock solution of 5 x 10-5 M Chan (1994) showed that there is a secretion of acinar J Physiol. 485.…”

Section: Biochemical Analysesmentioning

confidence: 99%

Differential secretion of proteins by rat submandibular acini and granular ducts on graded autonomic nerve stimulations.

Anderson

Garrett

Zhang

et al. 1995

The Journal of Physiology

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A fluorometric assay of peroxidase activity utilizing 2′,7′-dichlorofluorescein with thiocyanate: application to the study of salivary secretion

Cited by 33 publications

References 19 publications

Enzyme Activities in the Oral Fluids of Patients Suffering from Bulimia: A Controlled Clinical Trial

Enzyme Activities in the Oral Fluids of Patients Suffering from Bulimia: A Controlled Clinical Trial

Immunoglobulin A secretion into saliva during dual sympathetic and parasympathetic nerve stimulation of rat submandibular glands

Differential secretion of proteins by rat submandibular acini and granular ducts on graded autonomic nerve stimulations.

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