A fluorometric method for the estimation of 6-mercaptopurine in serum

Finkel, Joe M.

doi:10.1016/0003-2697(67)90311-9

Cited by 43 publications

(9 citation statements)

References 4 publications

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“…The adsorption experiments indicated that MIPs have good recognition properties for 6-mercaptopurine and thioguanine [134]. Obtained data on the levels of these drugs in plasma or serum can give valuable guidelines to more effective therapy [135].…”

Section: Mips and Cancermentioning

confidence: 99%

Molecularly Imprinted Polymers in Biological Applications

et al. 2020

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Molecularly imprinted polymers (MIPs) are currently widely used and further developed for biological applications. The MIP synthesis procedure is a key process, and a wide variety of protocols exist. The templates that are used for imprinting vary from the smallest glycosylated glycan structures or even amino acids to whole proteins or bacteria. The low cost, quick preparation, stability and reproducibility have been highlighted as advantages of MIPs. The biological applications utilizing MIPs discussed here include enzyme-linked assays, sensors, in vivo applications, drug delivery, cancer diagnostics and more. Indeed, there are numerous examples of how MIPs can be used as recognition elements similar to natural antibodies.

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Section: Mips and Cancermentioning

confidence: 99%

Molecularly Imprinted Polymers in Biological Applications

et al. 2020

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“…There have been several previous attempts at measuring azathioprine or 6-MP in plasma (Hamilton & Elion, 1954;Finkel, 1967;Loo, Luce, Sullivan & Frei, 1968;Coffey, White, Lesk, Rogers & Serpick, 1972;Spreafico, Donelli, Bossi, Wecchi, Standen & Garattini, 1973;Bailey, Wilson & Johnson, 1975) but these assays have either lacked specificity or sensitivity or both and have been of little value in studying azathioprine pharmacokinetics in patients. The present assay for 6-MP is the first to be developed with sufficient sensitivity and specificity to allow kinetic studies to be done on patients taking therapeutic doses of azathioprine (Maddocks, 1975).…”

Section: Discussionmentioning

confidence: 99%

“…As an anti-rejection drug, azathioprine is inconsistent in its effect and transplant rejection and drug toxicity continue to be major problems. Indeed, the results of kidney transplantation have not improved over the last ten years despite greater experience in surgical technique, kidney preservation and tissue typing (Renal Transplant Registry, 1977 Principle of the assay Finkel (1967) described an assay for 6-MP involving the oxidation of its -SH group to form purine 6-sulphonate which is measured fluorimetrically. This assay is not sensitive enough to measure therapeutic levels of 6-MP in plasma because of very high plasma blank readings, especially with uraemic plasma, and it lacks specificity.…”

Section: Introduction Methodsmentioning

confidence: 99%

“…This assay is not sensitive enough to measure therapeutic levels of 6-MP in plasma because of very high plasma blank readings, especially with uraemic plasma, and it lacks specificity. In this study it has been modified and a new method of extracting 6-MP from plasma has been developed which overcomes the problems of Finkel's (1967) Extraction and estimation ofplasma 6-MP…”

Section: Introduction Methodsmentioning

confidence: 99%

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Assay of azathioprine, 6‐mercaptopurine and a novel thiopurine metabolite in human plasma.

Maddocks¹

1979

Brit J Clinical Pharma

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1 A simple, specific assay for 6-mercaptopurine (6-MP) in human plasma with a sensitivity of 10 ng/ml (66 nmol/1) has been developed. 2 6-MP was extracted directly from plasma into toluene using a novel extraction procedure. This involves conversion of 6-MP into a phenyl mercury derivative by its reaction with phenyl mercuric acetate in alkaline plasma and extracting into toluene. Back-extraction of the toluene layer with 0. IN HC1 regenerates 6-MP, which is then oxidised to purine-6-sulphonate and assayed fluorimetrically. 3 This assay has been modified to measure azathioprine and a new thiopurine metabolite in plasma. 4 In a kidney transplant patient given azathioprine, 50 mg i.v., conversion to 6-MP was rapid and the plasma half-life of 6-MP was 36 min. 5 These assays are suitable for studying the pharmacokinetics of azathioprine in patients with kidney transplants. The 6-MP assay should also prove useful for studying the pharmacokinetics of the drug in patients with leukaemia.

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“…In der Literatur werden einige Methoden beschrieben, 6-Mercaptopurin im Blur zu bestimmen [1,4,5,7,8,9]. Diese Nachweismethoden lassen jedoch Spezifitfit und eine ausreichende Nachweisgrenze vermissen, so dab sie ftir diese Belange als insuffizient erscheinen.…”

Section: Materials Und Methodeunclassified

Ein gaschromatographischer Nachweis von 6-Mercaptopurin im Serum von immunsupprimierten Transplantationspatienten

Wypior¹,

Schilling²,

Meyer³

et al. 1982

Res. Exp. Med.

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The knowledge of serum levels of azathioprine or its metabolite 6-mercaptopurine is important for the monitoring of the immunosuppressive therapy after kidney transplantation, especially when kidney function is low. For this reason, the work of Bailey et al. was modified. 6-Mercaptopurine was extracted from the serum with a mixture of isopropanol-ethylacetate (1:1 v:v), derivatized with trimethylaniliniumhydroxyde and measured gaschromatographically. Sulfhydryl-protecting reagents were added to prevent loss of substance. Oxyethyltheophylline was used as the internal standard. The extraction and clean-up method recovered 80% of the 6-mercaptopurine present, the lower limit of sensitivity was 20 ng/ml, i.e., ten times lower than that of Bailey et al. The method could be realized on eight patients treated with azathioprine, the half life of the drug was about 20-40 min within the first hour i.v. application.

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A fluorometric method for the estimation of 6-mercaptopurine in serum

Cited by 43 publications

References 4 publications

Molecularly Imprinted Polymers in Biological Applications

Molecularly Imprinted Polymers in Biological Applications

Assay of azathioprine, 6‐mercaptopurine and a novel thiopurine metabolite in human plasma.

Ein gaschromatographischer Nachweis von 6-Mercaptopurin im Serum von immunsupprimierten Transplantationspatienten

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