A Fluorometric Micro Method for Serum Tocopherol

Hansen, Leland G.; Warwick, Warren J.

doi:10.1093/ajcp/46.1_ts.133

Cited by 81 publications

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“…Vitamin E concentration in LDL was determined by a micro-fluorometric method as described by Hansen and Warwick (31). Total cholesterol was determined by an enzymatic …”

Section: Methodsmentioning

confidence: 99%

Serum low density lipoprotein of alcoholic patients is chemically modified in vivo and induces apolipoprotein E synthesis by macrophages.

Lin

Dai²,

Lumeng³

et al. 1995

J. Clin. Invest.

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This work was carried out to investigate the effect of alcohol drinking on serum LDL. Agarose gel electrophoresis showed that LDL samples from alcoholic patients without serious liver disease were more negatively charged and moved faster toward the cathode than LDL from nondrinking control subjects. Rabbit antibodies raised by using keyhole limpet hemocyanin modified in vitro by 4-hydroxynonenal or by acetaldehyde as immunogens reacted more strongly with patients' LDL than with control LDL, indicating the presence of oxidatively modified epitopes and acetaldehyde adducts in alcoholic patients' LDL. LDL of alcoholic patients has decreased vitamin E contents. The electromobility of LDL decreased after abstinence from alcohol and returned to normal in 2 wk, but this was not accompanied by a significant increase in its vitamin E contents. When incubated with mouse peritoneal macrophages, patients' LDL induced apolipoprotein E secretion by threefold over control LDL with a concomitant increase in cellular cholesterol. Our results thus demonstrate that LDL of alcoholic patients has lower vitamin E content, is chemically modified in vivo, and exhibits altered biological function. These changes in heavy alcoholic drinkers may render LDL more atherogenic and thereby may counter the antiatherosclerosis effects of moderate alcohol consumption. (J. Clin. Invest. 1995Invest. . 95:1979Invest. -1986

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“…Vitamin E concentration in LDL was determined by a micro-fluorometric method as described by Hansen and Warwick (31). Total cholesterol was determined by an enzymatic …”

Section: Methodsmentioning

confidence: 99%

Serum low density lipoprotein of alcoholic patients is chemically modified in vivo and induces apolipoprotein E synthesis by macrophages.

Lin

Dai²,

Lumeng³

et al. 1995

J. Clin. Invest.

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“…ΚΤΗΝ. ΕΤΑΙΡΕΙΑΣ 2000, 51 (4) http://epublishing.ekt.gr | e-Publisher: EKT | Downloaded at 12/05/2018 01:18:38 | γκεντρώσεις σεληνίου, κατά 18% είχαν οριακές και μόνο κατά 9% φυσιολογικές, αν ληφθούν υπόψη οι συγκεντρώ σεις σεληνίου ήπατος, που θεωρούνται περισσότερο εν δεικτικές για την εκτίμηση της εν λόγω κατάστασης. Άλλη έρευνα για τις συγκεντρώσεις σεληνίου στα βοοειδή της χώρας μας δεν έχει γίνει, σε ανάλογη έρευνα των συγκε ντρώσεων σεληνίου σε ποίμνια αιγοπροβάτων της Θεσ σαλίας το 50% των προβάτων και αρνιών, το 75% των αι γών και το 100% των αιγιδίων είχαν πενικες συγκεντρώ σεις σεληνίου αίματος (< 0,075 μg/ml) 6 .…”

Section: συζητησηunclassified

“…ΚΤΗΝ. ΕΤΑΙΡΕΙΑΣ 2000, 51 (4) επίπεδων σεληνίου και βιταμίνης Ε, προκύπτει ότι σε ένα σημαντικό ποσοστό τα βοοειδή του νομοΰ Θεσσαλονίκης παρουσιάζουν πρόβλημα πενικών συγκεντρώσεων σελη νίου, ενώ φαίνεται πως μόνο σε ένα μικρό αριθμό τα ζώα αυτά αντιμετωπίζουν κίνδυνο αβιταμίνωσης Ε. Πρέπει να επισημανθεί, σε ό,τι αφορά το σελήνιο, ότι και σε αυτή την έρευνα έχουν καταγραφεί περιπτώσεις βοοειδών με ιδι αίτερα χαμηλές συγκεντρώσεις του εν λόγω στοιχείου, που είναι της τάξης 0,01 μg/ml αίματος.…”

Section: συζητησηunclassified

Epizootiologic survey of selenium and vitamin E concentrations in cattle to be slaughtered in Thessaloniki

Christodoulopoulos¹,

Roubies²,

Karatzias³

et al. 2018

J Hellenic Vet Med Soc

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The purpose of this survey was the study of selenium (Se) and vitamin E (vit. E) concentrations in cattle to be slaughtered in the area of Thessaloniki. For this purpose, research samples of blood and liver were collected from 205 cattle brought to different slaughter-houses of Thessaloniki. Out of the 205 cattle to be slaughtered, 78% presented deficient concentration of Se in liver (0,110-0,600 μg/g DM), 17% marginally deficient concentration(0,601-0,900 μg/g DM) and only 5% normal concentration (0,901-1,512 μg/g DM). Regarding vit. E, only 5% out of the 205 cattle to be slaughtered presented deficient concentration in liver (<5 μg/g WW). It is concluded from the above that, in Thessaloniki, a significant percentage of cattle run the risk of Se deficiency diseases. On the contrary, the case of vit. E deficiency, should be regarded as improbable for these animals.

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“…αυτές με α/α: 1,4,6,8,9,10,12,14,15,16,22,33,34,37,43,49,52,59, 60, 62 και 63 (πίνακας 2), είχαν γενικό μέσο όρο συ γκέντρωσης σεληνίου αίματος περίπου 0,05 ^ig/ml. Τέτοι ες συγκεντρώσεις σεληνίου στα βοοειδή, ιδιαίτερα όταν συνδυάζονται με ικανοποιητικές συγκεντρώσεις βιταμί νης Ε (>2 μg/ml πλάσματος) θείυρούνται υπεύθυνες συ νήθως μόνο για αύξηση της συχνότητας μα στ ίτ ίδιον, αύ ξηση της συχνότητας κατακράτησης εμβρυϊκιον υ μένουν και μείιοση της ανοσολογικής ανταπόκρισης του οργανι σμού 1 * 3^2 .…”

Section: συζητησηunclassified

Epizootiologic survey of selenium and vitamin E concentrations in dairy cows of Thessaioniki’s county

Christodoulopoulos¹,

Roubies²,

Karatzias³

et al. 2018

J Hellenic Vet Med Soc

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The purpose of this research was the study of selenium (Se) and vitamin E (vit. E) concentrations in dairy cows blood within the region of Thessaloniki. The survey included 65 dairy cow farms in the region of Thessaloniki from which samples of blood were collected from 10 female adult animals from every farm. Out of the total 650 cows examined in the 65 farms, 40% presented deficient concentration of Se in blood (<0,08 μg/ml), 29% marginally deficient concentration (0,08-0,12 μg/ml) and only 31% normal concentration (>0,12 μg/ml). Regarding vit. E, only 4%, out of the 650 cows examined, had deficient concentration in the blood plasma (<2 μg/ml). It was concluded from the above that, in the region of Thessaloniki, a significant percentage of the dairy cows run the risk of Se deficiency diseases. On the contrary, the case of vit. E deficiency, should be regarded as improbable for these animals.

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A Fluorometric Micro Method for Serum Tocopherol

Cited by 81 publications

References 0 publications

Serum low density lipoprotein of alcoholic patients is chemically modified in vivo and induces apolipoprotein E synthesis by macrophages.

Serum low density lipoprotein of alcoholic patients is chemically modified in vivo and induces apolipoprotein E synthesis by macrophages.

Epizootiologic survey of selenium and vitamin E concentrations in cattle to be slaughtered in Thessaloniki

Epizootiologic survey of selenium and vitamin E concentrations in dairy cows of Thessaioniki’s county

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