A Fluorometric Micromethod for Serum Vitamin A

Hansen, Leland G.; Warwick, Warren J.

doi:10.1093/ajcp/50.4_ts.525

Cited by 54 publications

(26 citation statements)

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“…The results therefore provide no evidence that abnormalities of vitamin A status are directly related to cancer (e.g., by being a precursor or a consequence). Vitamin A in the serum was measured by the fluorometric procedure of Hansen and Warwick [13], which has been reported to measure not only vitamin A but also phytofluene, a non-vitamin A component [ 17] . Hence, it may be argued that the validity of the conclusions based on the serum vitamin A levels is questionable.…”

Section: Discussionmentioning

confidence: 99%

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Biochemical Status of Vitamin A in Colorectal Cancer.

Basu

Hodgson

Temple

et al. 1991

J. Clin. Biochem. Nutr.

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SummarySerum There is considerable evidence from case-control studies that a low serum level of vitamin A is an important risk factor for the development of a variety of human cancers of epithelial cell origin [1][2][3][4][5][6][7][8]. Several of these studies [1,[4][5][6]8 ] have also indicated that the presence of an established cancer is associated with low serum concentrations of the vitamin A transport proteins, which include retinol-binding protein (RBP) and prealbumin. Furthermore, in our recent study [9] where serum vitamin A and RBP levels were measured in patients with surgically resected cancers of the colon and rectum, those who subsequently had recurrent disease showed lower levels of vitamin A and RBP compared with those who remained free of recurrence. Low RBP levels in plasma have also been reported in patients with recurrence of breast tumors, when compared with the

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Section: Discussionmentioning

confidence: 99%

“…Vitamin A was determined in the sera by a modification [12] of the fluorometric method of Hansen and Warwick [13]. RBP and prealbumin were determined by the single radial immunodiffusion technique [14] using LC-partigen immunodiffusion plates (Behring Diagnostic, Marburg, FRG).…”

Section: Methodsmentioning

confidence: 99%

Biochemical Status of Vitamin A in Colorectal Cancer.

Basu

Hodgson

Temple

et al. 1991

J. Clin. Biochem. Nutr.

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“…In the inpatient studies, before vitamin E therapy, breath ethane was obtained before (fasting) and 2 h after a breakfast high in carbohydrate (60% of total calories) or in fat (40% of total calories). Breath ethane and serum vitamin E (12), total lipids (9, 10) and erythrocyte peroxide hemolysis (1 1 [1][2][3][4][5] or PBS (tubes 6 and 7) was added to the erythrocyte suspension, and the mixture was incubated for 3 h at 37°C. PBS (4.5 mL) was added to tubes 1-3 (test) and tubes 6 and 7 (blank).…”

Section: Methodsmentioning

confidence: 99%

Utility of Breath Ethane as a Noninvasive Biomarker of Vitamin E Status in Children

et al. 1991

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ABSTRACT. The purpose of our study was to determine if the ethane content of expired air could be a useful index of vitamin E status in children. Eight children with vitamin E deficiency secondary to chronic severe liver disease were studied: six of these children were treated with parenteral vitamin E (2-5 mg/kg/dose every 4-7 d). Measures of vitamin E status pre-and posttherapy were: serum vitamin

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“…Both tissue and serum samples were read at 532 nm and were reported as nmol/g tissue or nmol/mL, respectively. The tissue and serum α-tocopherol concentrations were determined by fluorometric methods (Hitachi F-2000 fluorometer) with the samples read at excitation λ 290 nm and emission λ 329 nm (19,20), and they were expressed in µg/g tissue or µg/mL, respectively. Serum, total cholesterol, and triglycerides concentrations were measured enzymatically (Flexor E, Vital Scientific, the Netherlands) and reported as mmol/L.…”

Section: Biochemical Estimationsmentioning

confidence: 99%