A Fluorometric Micromethod for Serum Vitamins A and E

Hansen, Leland G.; Warwick, Warren J.

doi:10.1093/ajcp/51.4_ts.538

Cited by 117 publications

(21 citation statements)

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“…Serum vitamin E was estimated by a modified method of Hansen and Warwick, [25]. In a cover tube, 140 μL of Milli-Q water (Millipore, Bedford, MA, USA) was added to 20 μL of butylated hydroxytoluene 10 mM (BHT), 140 μL of sample and 2.1 mL of ethanol solution (66%).…”

Section: Serum Vitamin E Quantificationmentioning

confidence: 99%

Measurement of oxidative stress and antioxidant status in acute lymphoblastic leukemia patients

Battisti

Maders

Bagatini

et al. 2008

Clinical Biochemistry

132

135

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Section: Serum Vitamin E Quantificationmentioning

confidence: 99%

Measurement of oxidative stress and antioxidant status in acute lymphoblastic leukemia patients

Battisti

Maders

Bagatini

et al. 2008

Clinical Biochemistry

132

135

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“…The vitamin E status of each rat was confirmed at the time it was killed by measurement of serum vitamin E concentration by the fluorometric method (16), and in selected animals by measurement of vitamin E content in isolated hepatocytes.…”

Section: Animalsmentioning

confidence: 99%

Copper Toxicity and Lipid Peroxidation in Isolated Rat Hepatocytes: Effect of Vitamin E

et al. 1989

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ABSTRACT. We investigated the role of lipid peroxidation as the mechanism mediating copper toxicity in isolated rat hepatocytes and the modulating effect of vitamin E. Hepatocytes, isolated from rats fed diets containing deficient (E-), sufficient (E+), and excess (E++) amounts of vitamin E, were incubated with CuClz (0-2400 pM) for 150 min. Dose and time-dependent decreases in hepatocyte viability (determined by trypan blue exclusion and lactate dehydrogenase release) due to copper toxicity correlated with production of malonyldialdehyde in E-and E+ hepatocytes. However, malonyldialdehyde generation did not accompany copper toxicity in E++ cells. Copper toxicity was enhanced in E-compared to E+ and E++ hepatocytes as assessed by cell viability studies and ultrastructural plasma membrane bleb formation.

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“…The controls were given an equal volume of propylene glycol. Heparinised blood was collected by cardiac puncture and the liver, thy- [5]. Vitamin in the tissues was determined fluorometrically after saponification of weighed amounts of tissue homogenates with aqueous potassium hydrox ide in the presence of ethanolic pyrogallol [14], The fluorescence of the unsaponifiable matter was mea sured after selective extraction with hexane.…”

Section: Methodsmentioning

confidence: 99%

Effect of Corticosterone on the Plasma and Tissue Concentrations of Vitamin A in Rats

Atukorala

Basu

Dickerson³

1981

Ann Nutr Metab

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The administration of large doses of corticosterone to normal adult male rats resulted in a rapid loss of vitamin A from the plasma, liver, adrenals and thymus. Of the organs studied, the thymus appeared to be the most sensitive to treatment. The steroidmediated depression of plasma and tissue contents of vitamin A was reversed when animals were treated with corticosterone in combination with vitamin A.

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A Fluorometric Micromethod for Serum Vitamins A and E

Cited by 117 publications

References 0 publications

Measurement of oxidative stress and antioxidant status in acute lymphoblastic leukemia patients

Measurement of oxidative stress and antioxidant status in acute lymphoblastic leukemia patients

Copper Toxicity and Lipid Peroxidation in Isolated Rat Hepatocytes: Effect of Vitamin E

Effect of Corticosterone on the Plasma and Tissue Concentrations of Vitamin A in Rats

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