A fluorometric turn-on aptasensor for mucin 1 based on signal amplification via a hybridization chain reaction and the interaction between a luminescent ruthenium(II) complex and CdZnTeS quantum dots

Li, Zheng; Mao, Guobin; Du, Mingyuan; Songbai, Tian; Niu, Longqing; Ji, Xinghu; He, Zhike

doi:10.1007/s00604-019-3347-3

Cited by 25 publications

(12 citation statements)

References 30 publications

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“…They often couple with fluorescence quencher ruthenium complex (Ru(bpy) 2 (dppx) 2+ ) which can tightly bind toward dsDNA polymer including HCR product, causing the emission of QDs fluorescence. Based on this principle, Li et al developed a label-free aptamer biosensing assay for the detection of tumor biomarker mucin 1 (MUC1). This method achieved an LOD of 0.13 ng mL –1 and could be applied in spiked human serum samples.…”

Section: Hcr-based Biosensors With Nanomaterialsmentioning

confidence: 99%

“…One strategy is to initiate HCR by the release of complementary strand of aptamer. , Based on this principle, Zhang et al designed a facile fluorescent aptamer assay to distinguish specific cancer cells from normal cells through HCR engineered Cu metallization. The other strategy is to initiate or inhibit HCR by the structure-switching of aptamer. ,− Jia et al developed a label-free and sensitive assay for evaluating the activity and inhibition of protein kinase A (PKA) based on specific recognition of the aptamer and signal amplification of HCR with a low LOD of 1.5 U L –1 .…”

Section: Hcr-based Biosensors With Functional Dna Moleculesmentioning

confidence: 99%

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The Recent Development of Hybridization Chain Reaction Strategies in Biosensors

et al. 2020

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With the continuous development of biosensors, researchers have focused increasing attention on various signal amplification strategies to pursue superior performance for more applications. In comparison with other signal amplification strategies, hybridization chain reaction (HCR) as a powerful signal amplification technique shows its certain charm owing to nonenzymatic and isothermal features. Recently, on the basis of conventional HCR, this technique has been developed and improved rapidly, and a variety of HCR-based biosensors with excellent performance have been reported. Herein, we present a systematic and critical review on the research progress of HCR in biosensors in the last five years, including the newly developed HCR strategies such as multibranched HCR, migration HCR, localized HCR, in situ HCR, netlike HCR, and so on, as well as the combination strategies of HCR with isothermal signal amplification techniques, nanomaterials, and functional DNA molecules. By illustrating some representative works, we also summarize the advantage and challenge of HCR in biosensors, and offer a deep discussion of the latest progress and future development trends of HCR in biosensors.

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Section: Hcr-based Biosensors With Nanomaterialsmentioning

confidence: 99%

Section: Hcr-based Biosensors With Functional Dna Moleculesmentioning

confidence: 99%

The Recent Development of Hybridization Chain Reaction Strategies in Biosensors

et al. 2020

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“…DNA can protect Ru(bpy) 2 (dppx) 2+ from water due to the intercalation binding mode . The double helix of DNA can be intercalated by the dppx ligand of the Ru complex. , Such specific binding with a high combination constant ( K b = 1.5 × 10 5 M –1 ) is increasingly exploited in the development of turn-on sensors. − Herein, Ru(bpy) 2 (dppx) 2+ is utilized for the reason that it can efficiently quench the blue fluorescence of SiNDs as a result of the inner filter effect (IFE). ,, Using (3-aminopropyl)trimethoxysilane and trisodium citrate, blue-emitting SiNDs (spherical, about 2.5 nm, TEM characterization) can be easily prepared in a large scale (14 g) via a facile one-step hydrothermal method. , By simply mixing desired amounts of SiNDs and Ru(bpy) 2 (dppx) 2+ , a ratiometric sensor can be fabricated for DNA detection. In the presence of DNA, Ru(bpy) 2 (dppx) 2+ shows red fluorescence and the blue fluorescence of the quenched SiNDs remains unchanged (Scheme ).…”

Section: Introductionmentioning

confidence: 99%

Ratiometric Fluorescence Detection of DNA Based on the Inner Filter Effect of Ru(bpy)₂(dppx)²⁺ toward Silicon Nanodots

et al. 2020

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A ratiometric DNA sensor was developed based on fluorescent silicon nanodots (SiNDs) and Ru(bpy)2(dppx)2+. The absorption spectrum of Ru(bpy)2(dppx)2+ has significant overlap with both the excitation and emission spectra of SiNDs. Therefore, fluorescence quenching of Ru(bpy)2(dppx)2+ toward SiNDs can occur on account of the strong inner filter effect. The effect of quenching is not influenced by the specific binding between Ru(bpy)2(dppx)2+ and DNA. Fluorescence turn-on detection of DNA can be performed employing Ru(bpy)2(dppx)2+ and SiNDs as the response and reference signals, respectively. Using SiND–Ru(bpy)2(dppx)2+, a convenient, sensitive, rapid, and precise method could be developed for DNA detection. In aqueous solutions, the I 601/I 448 fluorescence intensity ratio of SiND–Ru(bpy)2(dppx)2+ increases linearly in the DNA concentration range of 20–1500 nM. The limit of detection and precision of the method is 4.3 nM and 3.5% (50 nM, n = 13), respectively. The ratiometric sensor was tested for visual detection of trace DNA. Moreover, this method was found suitable for the ratiometric detection of DNA in a simulated sample and a human serum sample, and the recoveries were in the range of 98–119%.

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“…Currently, a series of nucleic-acid-based amplification techniques have been developed. − The hybridization chain reaction (HCR), , as a simple and efficient isothermal amplification process without enzyme mediated, has been widely used in biosensing, bioimaging, and medicine applications as well as for the sensitive detection of nucleic acids, , proteins, metal ions, , and enzyme activities . In a typical HCR process, the concatenated hybridization of two hairpins is achieved by adding an initiator DNA to form a nicked double-strand DNA.…”

Section: Introductionmentioning

confidence: 99%

Multicolor and Ultrasensitive Enzyme-Linked Immunosorbent Assay Based on the Fluorescence Hybrid Chain Reaction for Simultaneous Detection of Pathogens

Huang²,

Liu

et al. 2019

J. Agric. Food Chem.

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Various pathogens may coexist in one sample; however, detection methods that rely on traditional selective culture media or immune agents designed specifically for a certain target are unsuitable for multiple targets. It is important to develop a simultaneous and sensitive detection method for multiple pathogens. Here, a multicolor and ultrasensitive enzymelinked immunosorbent assay (ELISA) platform based on the fluorescence hybridization chain reaction (HCR) was developed. In the assay, multicolor fluorescence concatemers formed as signal amplifiers and signal reporters in the presence of target pathogens. When HCR occurred, Escherichia coli O157:H7, Salmonella serotype Choleraesuis, and Listeria monocytogenes were detected simultaneously with three different fluorescences. Additionally, the limits of detection for E. coli O157:H7, Salmonella Choleraesuis, and L. monocytogenes were 3.4 × 10 1 , 6.4 × 10 0 , and 7.0 × 10 1 CFU/mL, respectively. The assay achieved ultrasensitive, specific, and simultaneous detection of three pathogens and can be applied to the detection of pathogens in milk samples. Therefore, this multicolor and ultrasensitive ELISA platform has great potential in the application of simultaneous detection of pathogens.

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A fluorometric turn-on aptasensor for mucin 1 based on signal amplification via a hybridization chain reaction and the interaction between a luminescent ruthenium(II) complex and CdZnTeS quantum dots

Cited by 25 publications

References 30 publications

The Recent Development of Hybridization Chain Reaction Strategies in Biosensors

The Recent Development of Hybridization Chain Reaction Strategies in Biosensors

Ratiometric Fluorescence Detection of DNA Based on the Inner Filter Effect of Ru(bpy)₂(dppx)²⁺ toward Silicon Nanodots

Multicolor and Ultrasensitive Enzyme-Linked Immunosorbent Assay Based on the Fluorescence Hybrid Chain Reaction for Simultaneous Detection of Pathogens

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A fluorometric turn-on aptasensor for mucin 1 based on signal amplification via a hybridization chain reaction and the interaction between a luminescent ruthenium(II) complex and CdZnTeS quantum dots

Cited by 25 publications

References 30 publications

The Recent Development of Hybridization Chain Reaction Strategies in Biosensors

The Recent Development of Hybridization Chain Reaction Strategies in Biosensors

Ratiometric Fluorescence Detection of DNA Based on the Inner Filter Effect of Ru(bpy)2(dppx)2+ toward Silicon Nanodots

Multicolor and Ultrasensitive Enzyme-Linked Immunosorbent Assay Based on the Fluorescence Hybrid Chain Reaction for Simultaneous Detection of Pathogens

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Ratiometric Fluorescence Detection of DNA Based on the Inner Filter Effect of Ru(bpy)₂(dppx)²⁺ toward Silicon Nanodots