A food grade approach for the isolation of major alkylresorcinols (ARs) from rye bran applying tailored supercritical carbon dioxide (scCO2) extraction combined with HPLC

Dey, Estera Szwajcer; Mikhailopulo, K. I.

doi:10.1016/j.supflu.2009.09.005

Cited by 16 publications

(16 citation statements)

References 24 publications

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“…TLC has been used a qualitative method for the identification and separation of ARs (42,157,160,171,172). In this method, a plate coated with a layer of silica gel or aluminum oxide, acts as the stationary phase.…”

Section: Thin Layer Chromatography (Tlc)mentioning

confidence: 99%

“…For a more in depth analysis of the AR homologue composition of extracts, either HPLC (39,110,157,(172)(173)(174)(175)(176)(177) and/or GC have been employed (38,42,90,111,112,156,157,167,168,174,(178)(179)(180)(181)(182)(183). HPLC separates, identifies, and quantifies AR homologues based on their characteristic retention times that are dependent on AR-chain length and degree of unsaturation.…”

Section: High Performance Liquid Chromatography (Hplc)mentioning

confidence: 99%

“…Given that the majority of AR homologues extracted from cereal grains are non-polar, a reversephase HPLC with a non-polar C18 column as the stationary phase and a polar solvent mobile phase is generally used (50,161,172,175,177). Use of a gradient system produces optimal results with clear peak separation points and reduced background noise in the resultant chromatogram; a combination of methanol and water (v/v) or a step-wise gradient of 70-99%…”

Section: High Performance Liquid Chromatography (Hplc)mentioning

confidence: 99%

“…MeOH have been commonly used (94,172). The characteristic retention times of the AR homologues are made available by a UV detector set at 280 nm (157,161,172,175) , a photodiode array (PDA) detector (61,174) or a coulometric electrode array detector (CEAD) (91,176) set at a scanning range of 250-350 nm. Other parameters such as the flow-rate of the mobile phase, column temperature, pump pressure, and the exact ratio of solvent gradient used varies depending on the specific system used.…”

Section: High Performance Liquid Chromatography (Hplc)mentioning

confidence: 99%

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Functional Properties of Triticale Bran Bioactives Using In Vitro and In Vivo Model Systems

Agil¹

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Part of the solution to reducing the prevalence of chronic diseases is the employment of food as medicine. Whole grain consumption has been associated with a variety of health benefits owing to their bioactive constituents, namely fibre and phytochemicals. The overall aim of this study was to discover the underlying mechanisms behind the positive physiological effects of whole grains and further elucidate their nutritional value. In particular, the bioactive properties of alkylresorcinols (ARs), phenolic lipids present almost exclusively in cereal grains, and soluble dietary fibre, potential prebiotics, were investigated using triticale bran (TB), an underestimated cereal crop.Specific objectives were met through the following projects. Conditions for large-scale extraction of ARs from cereal grains were optimized using response surface methodology (RSM). Isolation ofARs for 16-24 h at a solid-to-solvent ratio of 1:40 (weight per volume) and temperature of 24 °C produced the highest yield with the least amount of co-extractives/unknowns. The antioxidant and anti-inflammatory activities of ARs were evidenced by in vitro model systems, ORAC, DPPH, and COX assay whereby results were in agreement with ex vivo model systems, RAW 264.7 macrophage cell cultures induced by AAPH or LPS. Subsequently, the in vivo effects of ARs were evaluated and oxidative stress markers in liver and heart tissues of mice supplemented 0.5% ARs and mice fed 10% TB demonstrated antioxidant protection. Additionally, water extractable polysaccharides (WEP) from cereal samples were isolated by varying treatments;the highest yield with the least amount of impurities was achieved by boiling water extraction followed by successive enzyme treatments, dialysis and successive ethanol fractionations. Using yogurt as fermentation model, probiotic yogurt in the presence of TB produced significantly higher TTA values, lower pH levels, and improved microbial viability compared to controls. In addition to the prebiotic potential of TB, likely owing to its high fibre content, isolates of WEP also demonstrated antioxidant activity as measured by ORAC assay.First and foremost, I would like to thank my academic supervisor Dr. Farah Hosseinian for this incredible and life changing opportunity that has helped me learn and grow as a researcher, student, teacher, team player, and as an individual overall. I am very fortunate to have a supervisor like Dr. Hosseinian who encouraged me to work hard and push forth during trying times. She gave me confidence in my abilities, guidance, and shared all of her knowledge and wisdom while still challenging me to push myself beyond my perceived limits.

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Section: Thin Layer Chromatography (Tlc)mentioning

confidence: 99%

Section: High Performance Liquid Chromatography (Hplc)mentioning

confidence: 99%

Section: High Performance Liquid Chromatography (Hplc)mentioning

confidence: 99%

Section: High Performance Liquid Chromatography (Hplc)mentioning

confidence: 99%

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Functional Properties of Triticale Bran Bioactives Using In Vitro and In Vivo Model Systems

Agil¹

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“…Thin layer chromatography (TLC) and paper chromatography have been used for the identification and separation of ARs qualitatively. 45,[79][80][81] Cereal grains have been analyzed by TLC using silica gel or alumina on the plates. [82][83][84] After running the TLC plate it is stained with a staining reagent such as Fast Blue B to indicate the presence of any ARs.…”

Section: Thin Layer and Paper Chromatographymentioning

confidence: 99%

Extraction, Bioactivity, and Stability of Wheat Bran Alkylresorcinols

Gunenc¹

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Whole grain intake may be linked to a lowered risk of chronic diseases and such positive effects might be attributed to phenolic lipids, alkylresorcinols (ARs), found in cereal bran. This study aimed to characterize ARs in wheat bran (WB), investigate the influence of environmental factors on AR composition, measure AR bioactivity including antioxidant activity (AA) and investigate AR stability during baking. Moreover, the prebiotic potential of WB-soluble dietary fibre (SDF) was explored using yogurt models. Specific objectives were achieved by the following projects.The effects of cultivar, and region on the ARs content in 24 wheat cultivars grown in Ontario were studied, by GC-MS and their AA were evaluated. TPC (3.0 to 58.0 mg FAE/g), DPPH (5 to 68%), ORAC (6.0 to 94.0 µmol TE/g) and ARs (21.0 to 1522.0 μg/g) of WB extracts were significantly affected by location and cultivar (P < 0.05).The stability of WB-ARs was studied using different bread formulations. Bread ARs (1.1 to 82.9 mg/100 g) were heat stable during baking at 255 °C for 12 min. A positive correlation was observed between TPC and ORAC (R 2 = 0.90).Two extraction methods were used to compare the AR contents of WB. The % ARs per extract using acetone versus SC-CO 2 extraction of both WB samples studied were in the range of 10.9% -15.6% and 5.1% -6.6%, respectively.The prebiotic potential of WB-SDF to enhance lactic acid bacteria (LAB) survival in yogurt models was explored. Total DF (53%) was counted as a sum of SDF (6%) and IDF (47%). HPLC analysis of DF fractions treated with alkaline hydrolysis showed that IDF (84.2%) had a higher phenolic acid (PA) content than SDF (15.8%). There was a significant difference in total bacterial count (9.1 log CFU/mL), pH (4.8) and TTA iii (1.4%) in yogurt samples containing 4% WB compared to controls during the four week cold storage period (4 °C).These results could lead to practical applications of WB-ARs and SDF in the functional foods and nutraceutical industry. Furthermore, increased WB utilization will reduce Agri-Food leftovers, thus improving environmental sustainability.iv

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Supercritical fluid extraction of wheat bran oil: Study of extraction yield and oil quality

Rebolleda

Beltrán

Sanz

et al. 2014

Euro J Lipid Sci & Tech

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The supercritical fluid extraction (SFE) of wheat bran oil with pure supercritical carbon dioxide at different extraction pressure (25, 40 and 55 MPa) and temperature (40, 70 and 95 ºC) has been studied. Since wheat bran is characterized by having an important content of bioactive compounds such as alkylresorcinols (AR) and other phenolic compounds, the content of such compounds in the extracted oil was evaluated in order to establish oil quality. The influence of extraction pressure and temperature and wheat bran moisture on the extraction yield and oil quality was studied. Oil quality was evaluated through parameters such as AR content and profile, total polyphenols index (TPI), antioxidant activity and fatty acids profile. SFE performed over fresh wheat bran at 55 MPa and 95 ºC was found to provide wheat bran oil with the highest AR and phenolic content and antioxidant capacity of all the runs performed in this work. Some SFE experiments focused on oil fractionation showed that this technique can provide oil fractions enriched with AR and phenolic compounds.

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A food grade approach for the isolation of major alkylresorcinols (ARs) from rye bran applying tailored supercritical carbon dioxide (scCO2) extraction combined with HPLC

Cited by 16 publications

References 24 publications

Functional Properties of Triticale Bran Bioactives Using In Vitro and In Vivo Model Systems

Functional Properties of Triticale Bran Bioactives Using In Vitro and In Vivo Model Systems

Extraction, Bioactivity, and Stability of Wheat Bran Alkylresorcinols

Supercritical fluid extraction of wheat bran oil: Study of extraction yield and oil quality

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