A footprint of plant eco-geographic adaptation on the composition of the barley rhizosphere bacterial microbiota

Terrazas, Rodrigo Alegria; Balbirnie-Cumming, Katharin; Morris, Jenny; Hedley, Pete E.; Russell, Joanne; Paterson, Eric; Baggs, Elizabeth M.; Fridman, Eyal; Bulgarelli, Davide

doi:10.1038/s41598-020-69672-x

Cited by 54 publications

(42 citation statements)

References 80 publications

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“…The effect of geographical location on the microbiome has been discovered in other plants such as barley (Terrazas et al, 2020) and Gymnadenia conopsea (Lin et al, 2020), and significant variation has been reported. Each location in the present study harbored unique bacteria that could be used as a geographical indicator.…”

Section: Microbial Genera Specific To Geographical Locationmentioning

confidence: 99%

Microbiome Fingerprint as Biomarker for Geographical Origin and Heredity in Crocus sativus: A Feasibility Study

Bhagat

Sharma

Ambardar

et al. 2021

Front. Sustain. Food Syst.

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Host–microbiome interactions are specific and not random, making them defining entities for the host. The hypothesis proposed by various researchers earlier, that both plants and animals harbor specific inheritable core microbiome, is being augmented in the present study. Additionally, a case for using microbial fingerprint as a biomarker, not only for plant identification but also as a geographical indicator, has been investigated, taking Crocus sativus, saffron, as a study material. Crocus sativus, a monogenetic herb, on account of its male sterility and vegetative propagation, is reported to lack genome based molecular markers. Cormosphere microbiome (microbiome associated with corm) has been compared across three geographical locations, in two continents, to identify the core and unique microbiome, during the vegetative phase of its growth. Microbiome analysis done at phylum and genus level, using next generation sequencing technology, revealed that cormosphere at three locations harbored common phyla. At genus level, 24 genera were found common to all three geographical locations, indicating them to be part of the core microbiome of saffron. However, there were some bacterial genera unique to Kashmir, Kishtwar, and Morocco that can be used to develop microbial markers/geographical indicators for saffron grown in these regions. This is a preliminary study, indicating that the location specific bacterial community can be used to develop microbial barcodes but needs further augmentation with high coverage data from other saffron growing geographical regions.

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Section: Microbial Genera Specific To Geographical Locationmentioning

confidence: 99%

Microbiome Fingerprint as Biomarker for Geographical Origin and Heredity in Crocus sativus: A Feasibility Study

Bhagat

Sharma

Ambardar

et al. 2021

Front. Sustain. Food Syst.

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“…For seed germination, seeds were placed on petri dishes containing semi-solid agar media at 0.5 % concentration. After a week, seedlings displaying a comparable development were individually transferred into 12-cm pots containing approximately 500 g of Quarryfield agricultural soil (Robertson-Albertyn et al, 2017;Alegria Terrazas et al, 2020) previously sieved to remove stones and large debris. Plants were grown in a randomized design in a glasshouse at 18/14°C (day/night) temperature regime with 16 h daylight that was supplemented with artificial lighting to maintain a minimum light intensity of 200 μ mol quanta m -2 s -1 .…”

Section: Growth Conditionsmentioning

confidence: 99%

“…Sequencing reads were processed using a customized bioinformatics pipeline as described before (Alegria Terrazas et al, 2020). Briefly:…”

Section: Amplicon Sequencing Reads Processingmentioning

confidence: 99%

“…As wild ancestors of modern cultivated varieties may hold the capacity to adapt to marginal soil conditions, there is a growing interest in discerning the molecular mechanisms underpinning microbiota recruitment in crop wild relatives and their contribution to plant's adaptation to the environment (Escudero-Martinez & Bulgarelli, 2019). This is particularly attractive for crops like barley (Hordeum vulgare), the fourth most cultivated cereal worldwide, for which modern and wild genotypes are readily available for experimentation (Bulgarelli et al, 2013;Alegria Terrazas et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

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Applications of the indole-alkaloid gramine modulate the assembly of individual members of the barley rhizosphere microbiota

Maver

Morris

Hedley

et al. 2020

Preprint

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The biosynthesis of plant allelochemicals underpinning inter-organismal relationships has been moulded by domestication and breeding selection. The indole-alkaloid gramine, whose occurrence in barley (Hordeum vulgare L.) is widespread among wild genotypes but virtually absent from modern varieties, is a paradigmatic example of this phenomenon. This prompted us to investigate how the exogenous application of gramine impacted on the rhizosphere bacterial microbiota of two, gramine-free, elite barley varieties grown in a reference agricultural soil. Our investigation revealed that the application of the indole-alkaloid gramine modulates the proliferation of a subset of soil bacteria with a relatively broad phylogenetic assignment. This effect is two-pronged: a limited, but significant, component of the barley microbiota responds to gramine application in a genotype- and dosage-independent manner while at the highest dosage this secondary metabolite attenuates the host recruitment cues of the barley microbiota. Interestingly, this latter effect displayed a bias for members of the phyla Proteobacteria. These initial observations indicate that gramine can act as a determinant of the bacterial communities inhabiting the root-soil interface.

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“…Another interesting strategy is to incorporate the microbiome in breeding ( Wei and Jousset, 2017 ). There are several examples from cereal crops demonstrating the impact of breeding on the root and rhizosphere microbiome ( Alegria Terrazas et al, 2020 ; Kinnunen-Grubb et al, 2020 ). Valente et al (2020) showed that root colonization by Pseudomonas kilonensis , a plant growth promoting rhizobacterium (PGPR), was higher in ancient wheat cultivars than in modern ones under gnotobiotic conditions.…”

Section: Toward Microbiome-based Management Of Fhbmentioning

confidence: 99%

Fusarium Head Blight From a Microbiome Perspective

2021

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The fungal genus Fusarium causes several diseases in cereals, including Fusarium head blight (FHB). A number of Fusarium species are involved in disease development and mycotoxin contamination. Lately, the importance of interactions between plant pathogens and the plant microbiome has been increasingly recognized. In this review, we address the significance of the cereal microbiome for the development of Fusarium-related diseases. Fusarium fungi may interact with the host microbiome at multiple stages during their life cycles and in different plant organs including roots, stems, leaves, heads, and crop residues. There are interactions between Fusarium and other fungi and bacteria as well as among Fusarium species. Recent studies have provided a map of the cereal microbiome and revealed how different biotic and abiotic factors drive microbiome assembly. This review synthesizes the current understanding of the cereal microbiome and the implications for Fusarium infection, FHB development, disease control, and mycotoxin contamination. Although annual and regional variations in predominant species are significant, much research has focused on Fusarium graminearum. Surveying the total Fusarium community in environmental samples is now facilitated with novel metabarcoding methods. Further, infection with multiple Fusarium species has been shown to affect disease severity and mycotoxin contamination. A better mechanistic understanding of such multiple infections is necessary to be able to predict the outcome in terms of disease development and mycotoxin production. The knowledge on the composition of the cereal microbiome under different environmental and agricultural conditions is growing. Future studies are needed to clearly link microbiome structure to Fusarium suppression in order to develop novel disease management strategies for example based on conservation biological control approaches.

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A footprint of plant eco-geographic adaptation on the composition of the barley rhizosphere bacterial microbiota

Cited by 54 publications

References 80 publications

Microbiome Fingerprint as Biomarker for Geographical Origin and Heredity in Crocus sativus: A Feasibility Study

Microbiome Fingerprint as Biomarker for Geographical Origin and Heredity in Crocus sativus: A Feasibility Study

Applications of the indole-alkaloid gramine modulate the assembly of individual members of the barley rhizosphere microbiota

Fusarium Head Blight From a Microbiome Perspective

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