A forensic case study for body fluid identification using DNA methylation analysis

Choung, Chong Min; Lee, Jee Won; Park, Ji Hye; Kim, Cho Hee; Park, Hyun-Chul; Lim, Sung-Soo

doi:10.1016/j.legalmed.2021.101872

Cited by 12 publications

(2 citation statements)

References 17 publications

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“…This can be achieved when the DNA extract is used for cell type inference (as with methylation analysis) or when RNA is extracted from a fraction normally discarded during DNA extraction (like a flow through fraction when binding DNA to a silica column). Case reports have been published for both methylation [298] and RNA-based assays [299]. The advantage of using DNA for body fluid typing is that no additional extraction procedure is needed; the disadvantage is that less DNA remains for further analyses such as Y-STR profiling, mtDNA analysis or the prediction of age, ancestry, appearance.…”

Section: Discussionmentioning

confidence: 99%

On the Identification of Body Fluids and Tissues: A Crucial Link in the Investigation and Solution of Crime

Sijen

Harbison

2021

Genes

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Body fluid and body tissue identification are important in forensic science as they can provide key evidence in a criminal investigation and may assist the court in reaching conclusions. Establishing a link between identifying the fluid or tissue and the DNA profile adds further weight to this evidence. Many forensic laboratories retain techniques for the identification of biological fluids that have been widely used for some time. More recently, many different biomarkers and technologies have been proposed for identification of body fluids and tissues of forensic relevance some of which are now used in forensic casework. Here, we summarize the role of body fluid/ tissue identification in the evaluation of forensic evidence, describe how such evidence is detected at the crime scene and in the laboratory, elaborate different technologies available to do this, and reflect real life experiences. We explain how, by including this information, crucial links can be made to aid in the investigation and solution of crime.

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Section: Discussionmentioning

confidence: 99%

On the Identification of Body Fluids and Tissues: A Crucial Link in the Investigation and Solution of Crime

Sijen

Harbison

2021

Genes

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show abstract

“…A number of approaches are being taken to improve the sensitivity and specificity of BFID in recent years including DNA methylation [ [148] , [149] , [150] , [151] , [152] , [153] , [154] , [155] , [156] , [157] , [158] , [159] , [160] , [161] ], messenger RNA (mRNA) [ [162] , [163] , [164] , [165] , [166] ], microRNA (miRNA) [ 167 ], protein mass spectrometry for seminal fluid detection [ 168 ], and microbiome analysis [ 169 , 170 ]. Although many new techniques are being described in the scientific literature, traditional methods for semen identification are still widely used in regular forensic casework [ 171 ].…”

Section: Advancements In Current Practicesmentioning

confidence: 99%

Recent advances in forensic biology and forensic DNA typing: INTERPOL review 2019–2022

Butler

2023

Forensic Science International: Synergy

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Use of Advanced Molecular Techniques for Human Body Fluids Detection

Dash,

Elkins,

Al-Snan

2023

Advancements in Forensic DNA Analysis

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A forensic case study for body fluid identification using DNA methylation analysis

Cited by 12 publications

References 17 publications

On the Identification of Body Fluids and Tissues: A Crucial Link in the Investigation and Solution of Crime

On the Identification of Body Fluids and Tissues: A Crucial Link in the Investigation and Solution of Crime

Recent advances in forensic biology and forensic DNA typing: INTERPOL review 2019–2022

Use of Advanced Molecular Techniques for Human Body Fluids Detection

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