A forward reconstruction, holographic method to overcome the lens effect during 3D detection of semi-transparent, non-spherical particles

Tai, Cheng-Wei; Ahmadzadegan, Adib; Ardekani, Arezoo M.; Narsimhan, Vivek

doi:10.1039/d2sm00738j

Cited by 1 publication

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“…Statistical comparisons were made on the 3D swimming speed and turning angle distributions of P. aeruginosa , Agrobacterium tumefaciens , and Escherichia coli ( E. coli ) [ 159 ]. Submicron-scale kinematics, morphological shape, and orientation measurements were conducted on individual E. coli to assess their motilities, including swimming speed, tumbling motion, and wobbling motion [ 62 , 80 , 160 ]. The body-angle rotation during runs, tumbles, and pole reversal in E. coli was measured using a C-implemented discrete dipole approximation code and the Levenberg-Marquardt algorithm [ 161 ].…”

Section: Applications Of Dihm To Various Microscale Biological Cellsmentioning

confidence: 99%

Digital in-line holographic microscopy for label-free identification and tracking of biological cells

Kim,

Lee

2024

Military Med Res

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Digital in-line holographic microscopy (DIHM) is a non-invasive, real-time, label-free technique that captures three-dimensional (3D) positional, orientational, and morphological information from digital holographic images of living biological cells. Unlike conventional microscopies, the DIHM technique enables precise measurements of dynamic behaviors exhibited by living cells within a 3D volume. This review outlines the fundamental principles and comprehensive digital image processing procedures employed in DIHM-based cell tracking methods. In addition, recent applications of DIHM technique for label-free identification and digital tracking of various motile biological cells, including human blood cells, spermatozoa, diseased cells, and unicellular microorganisms, are thoroughly examined. Leveraging artificial intelligence has significantly enhanced both the speed and accuracy of digital image processing for cell tracking and identification. The quantitative data on cell morphology and dynamics captured by DIHM can effectively elucidate the underlying mechanisms governing various microbial behaviors and contribute to the accumulation of diagnostic databases and the development of clinical treatments.

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Section: Applications Of Dihm To Various Microscale Biological Cellsmentioning

confidence: 99%

Digital in-line holographic microscopy for label-free identification and tracking of biological cells

Kim,

Lee

2024

Military Med Res

View full text Add to dashboard Cite

show abstract

A forward reconstruction, holographic method to overcome the lens effect during 3D detection of semi-transparent, non-spherical particles

Abstract: Suspensions of semi-transparent particles such as polystyrene microparticles are commonly used as model systems in the study of micro-rheology, biology, and microfluidics. Holography is a valuable tool that allows one...

Cited by 1 publication

References 44 publications

Digital in-line holographic microscopy for label-free identification and tracking of biological cells

Digital in-line holographic microscopy for label-free identification and tracking of biological cells

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