2022
DOI: 10.1021/acssensors.2c02067
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A Four-Channel Surface Plasmon Resonance Sensor Functionalized Online for Simultaneous Detections of Anti-SARS-CoV-2 Antibody, Free Viral Particles, and Neutralized Viral Particles

Abstract: Current tests for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) detect either the constituent nucleic acids/proteins of the viral particles or antibodies specific to the virus, but cannot provide information about viral neutralization by an antibody and the efficacy of an antibody. Such information is important about individuals' vulnerability to severe symptoms or their likelihood of showing no symptoms. We immobilized online SARS-CoV-2 spike (S1) protein and angiotensin-converting enzyme 2 (AC… Show more

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Cited by 8 publications
(9 citation statements)
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“…[17][18][19][20][21][22][23] Quantitative determination of biomolecular interactions is a hallmark of SPR sensing, which has been used to determine antibody affinity 24,25 and other biomolecular binding information, 26,27 and these properties were valuable during the COVID-19 pandemic. Recently, a promising SPR platform was developed to detect antibodies, viral particles and neutralized viral particles, 28 providing an all-in-one sensor to address the key questions related to SARS-CoV-2 infection and the pandemic response. Similarly, SPR sensing has been applied to small molecule screening for discovery of putative therapeutic inhibitors such as peptides, 29 cannabinoids, 30 or other small molecule inhibitors.…”
Section: Introductionmentioning
confidence: 99%
“…[17][18][19][20][21][22][23] Quantitative determination of biomolecular interactions is a hallmark of SPR sensing, which has been used to determine antibody affinity 24,25 and other biomolecular binding information, 26,27 and these properties were valuable during the COVID-19 pandemic. Recently, a promising SPR platform was developed to detect antibodies, viral particles and neutralized viral particles, 28 providing an all-in-one sensor to address the key questions related to SARS-CoV-2 infection and the pandemic response. Similarly, SPR sensing has been applied to small molecule screening for discovery of putative therapeutic inhibitors such as peptides, 29 cannabinoids, 30 or other small molecule inhibitors.…”
Section: Introductionmentioning
confidence: 99%
“…Other sensing strategies have been investigated in pursuit of shorter assay times, increased throughput, and improved sensitivity. A flow-based chemiluminescence microarray immunoassay could detect nAbs in blood samples in just 7 min [15], while a four-channel SPR sensor enabled simultaneous detection of anti-S1 antibodies as well as free and neutralized virus particles [16]. The use of a Tri-part split-NanoLuc® facilitated a homogeneous neutralization test [17] and the use of Luminex beads holds the potential for multiplexing [18].…”
Section: Introductionmentioning
confidence: 99%
“…Quantitative determination of biomolecular interactions is a hallmark of SPR sensing, which has been used to determine antibody affinity 24,25 and other biomolecular binding information 26,27 , and these properties were valuable during the COVID-19 pandemic. Recently, a promising SPR platform was developed to detect antibodies, viral particles and neutralized viral particles 28 , providing an all-in-one sensor to address the key questions related to SARS-CoV-2 infection and the pandemic response.…”
Section: Introductionmentioning
confidence: 99%