A Fraction of Yeast Cu,Zn-Superoxide Dismutase and Its Metallochaperone, CCS, Localize to the Intermembrane Space of Mitochondria

Sturtz, Lori A.; Diekert, Kerstin; Jensen, Laran T.; Lill, Roland; Culotta, Valeria C.

doi:10.1074/jbc.m105296200

Cited by 616 publications

(126 citation statements)

References 68 publications

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“…• -and H 2 O 2 ) generated in this process are neutralized by enzymes such as superoxide dismutase (SOD) and catalase (CAT), thus protecting the cells from them [12]. The SOD family has three distinct members: SOD1 or copper/zinc SOD, SOD2 or manganese SOD, and SOD3 or extracellular SOD [12,14,15]. SOD1 is the major type of SOD in the cytoplasm, nucleus, and mitochondrial intermembrane space [12,15].…”

Section: Introductionmentioning

confidence: 99%

Hydroxychloroquine (HCQ) Modulates Autophagy and Oxidative DNA Damage Stress in Hepatocellular Carcinoma to Overcome Sorafenib Resistance via TLR9/SOD1/hsa-miR-30a-5p/Beclin-1 Axis

Chen

Yadav

Chu

et al. 2021

Cancers

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Sorafenib is used for treating advanced hepatocellular carcinoma (HCC), but some patients acquire sorafenib resistance. We investigated the mechanisms underlying acquired sorafenib resistance in HCC cells and targeted them to re-sensitize them to sorafenib. In silico analysis indicated that toll-like receptor (TLR)-9 was significantly overexpressed, and that miRNA (hsa-miR-30a-5p) was downregulated in sorafenib-resistant HCC cells, which modulated HCC cell proliferation, oxidative stress, and apoptosis. TLR9 overexpression increased HCC cell proliferation, whereas TLR9 inhibition from hydroxychloroquine (HCQ) decreased HCC cell proliferation, tumor growth, oxidative stress marker (SOD1), and the formation of autophagosome bodies (reduced ATG5 and Beclin-1 expression). Moreover, HCQ treatment reduced epithelial–mesenchymal transition, leading to decreased clonogenicity, migratory ability, and invasiveness. HCQ targeted and reduced the self-renewal capacity phenotype by inhibiting tumorsphere generation. Both in vitro and in vivo results demonstrated the synergistic effect of the HCQ–sorafenib combination on sorafenib-resistant HCC (Huh7-SR) cells, increasing their sensitivity to treatment by modulating TLR9, autophagy (ATG5 and Beclin-1), oxidative stress (SOD1), and apoptosis (c-caspase3) expression and thus overcoming the drug resistance. This study’s findings indicate that TLR9 overexpression occurs in sorafenib-resistant HCC cells and that its downregulation aids HCC suppression. Moreover, HCQ treatment significantly increases sorafenib’s effect on sorafenib-resistant HCC cells.

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Section: Introductionmentioning

confidence: 99%

Hydroxychloroquine (HCQ) Modulates Autophagy and Oxidative DNA Damage Stress in Hepatocellular Carcinoma to Overcome Sorafenib Resistance via TLR9/SOD1/hsa-miR-30a-5p/Beclin-1 Axis

Chen

Yadav

Chu

et al. 2021

Cancers

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“…Hepatic MT expression may be an indicator of Zn bioavailability, given that local Zn levels dictate MT mRNA levels ( Wang et al., 2012 ). The SOD is an enzyme that uses Cu or Zn as cofactors and protects cells from oxidative damage by eliminating superoxide anion radicals ( Sturtz et al, 2001 ; Miao and St. Clair, 2009 ). Hepatic SOD mRNA expression was negatively associated with Cu intake and the overall status in cattle ( Hansen et al, 2009 ).…”

Section: Resultsmentioning

confidence: 99%

Supplementing organic-complexed or inorganic Co, Cu, Mn, and Zn to beef cows during gestation: physiological and productive response of cows and their offspring until weaning

Harvey

Cooke

Colombo

et al. 2021

Journal of Animal Science

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One hundred and ninety non-lactating, pregnant beef cows (¾ Bos taurus and ¼ Bos indicus; 138 multiparous and 52 primiparous) were assigned to this experiment at 117 ± 2.2 d of gestation (d 0). Cows were ranked by parity, pregnancy type (artificial insemination = 102, natural service = 88), body weight (BW) and body condition score (BCS), and assigned to receive a supplement containing: 1) sulfate sources of Cu, Co, Mn, and Zn (INR; n = 95) or 2) an organic complexed source of Cu, Mn, Co, and Zn (AAC; Availa®4; Zinpro Corporation, Eden Prairie, MN; n = 95). The INR and AAC provided the same daily amount of Cu, Co, Mn, and Zn, based on 7 g of the AAC source. From d 0 to calving, cows were maintained in a single pasture and were segregated three times weekly into 1 of 24 individual feeding pens to receive treatments. Cow BW and BCS were recorded on d -30, d 97, upon calving, and at weaning (d 367). Milk production was estimated at 42 ± 0.5 d postpartum via weigh-suckle-weigh (WSW). Liver biopsies were performed in 30 cows/treatment on d -30, 97, upon calving, and the day after WSW. Calf BW was recorded at birth and weaning. Liver and longissimus muscle (LM) biopsies were performed in 30 calves/treatment upon calving and 24 h later, the day after WSW, and at weaning. No treatment effects were detected (P ≥ 0.49) for cow BCS during gestation, despite AAC cows having greater (P = 0.04) BW on d 97. Liver Co concentrations were greater (P < 0.01) for AAC compared to INR cows, and liver concentrations of Cu were greater (P = 0.02) for INR compared to AAC cows on d 97. Upon calving, INR cows had greater (P ≤ 0.01) liver Cu and Zn concentrations compared to AAC cows. No other treatment differences were noted (P ≥ 0.17) for cow and calf liver trace mineral concentrations. Cows receiving AAC had greater (P = 0.04) hepatic mRNA expression of metallothionein 1A at calving, and their calves had greater (P = 0.04) hepatic mRNA expression of superoxide dismutase at weaning. Milk production did not differ between AAC and INR cows (P = 0.70). No treatment effects were detected (P ≥ 0.29) for mRNA expression of LM genes associated with adipogenic or muscle development activities in calves at birth and weaning. Calf birth and weaning BW also did not differ (P ≥ 0.19) between treatments. In summary, supplementing Co, Cu, Zn, and Mn as organic complexed or sulfate sources to beef cows during the last 5 mo of gestation yielded similar cow-calf productive responses until weaning.

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“…•− to H 2 O 2 , and H 2 O 2 is subsequently detoxified to H 2 O and O 2 , catalyzed by catalase or glutathione peroxidase (Scandalios, 2005). Most eukaryotic cells express copper-zinc superoxide dismutases (CuZnSODs) in the cytoplasm (Sturtz et al, 2001) and manganese superoxide dismutases (MnSODs) in the mitochondrial matrix (Perry et al, 2010). Recently, the complete cDNA coding sequence of a host-derived CuZnSOD has been obtained from the outer mantle of the T. squamosa (Chew et al, 2020).…”

Section: The Outer Mantle Needs Light-enhanced Anti-oxidative Defense Mechanisms To Deal With O 2 Produced By Photosynthesizing Symbiontsmentioning

confidence: 99%

Light-Dependent Phenomena and Related Molecular Mechanisms in Giant Clam-Dinoflagellate Associations: A Review

Chew

2021

Front. Mar. Sci.

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Giant clams can grow to large sizes despite living in oligotrophic waters of the tropical Indo-Pacific as they maintain a mutualistic relationship with symbiotic dinoflagellates (zooxanthellae) and receive photosynthate from them. The phototrophic dinoflagellates live extracellularly inside a tubular system located mainly in the colorful outer mantle and have no access to the ambient seawater. Hence, the clam host needs to absorb exogenous inorganic carbon (Ci), nitrogen (N) and phosphorus (P), and supply them to the symbionts. As photosynthesizing symbionts need more nutrients in light than in the dark, the uptake rates of these exogenous nutrients by the host must increase during illumination, implying that the host’s transporters involved need to be regulated by some kind of light-responsive mechanisms. Furthermore, the growth and development of the host can also be augmented by light, because of the photosynthate donated by the photosynthesizing symbionts. Consequently, giant clams display many light-dependent phenomena related to phototrophy, antioxidative defense, biomineralization, as well as absorption of exogenous Ci, N, and P. These phenomena may involve collaborations among enzymes and transporters in several organs of the host, whereby the gene and protein expression levels of these biocatalysts are up- or down-regulated during illumination. This review aims to examine the molecular mechanisms of light-dependent physiological phenomena that occur in intact giant clam-dinoflagellate associations, and to highlight the differences between giant clams and scleractinian corals in those regards. As the population of giant clams in nature are dwindling due to climate change and anthropogenic activities, a good understanding of their light-dependent processes may generate new ideas to improve their growth and survival under rapidly changing environmental conditions.

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A Fraction of Yeast Cu,Zn-Superoxide Dismutase and Its Metallochaperone, CCS, Localize to the Intermembrane Space of Mitochondria

Cited by 616 publications

References 68 publications

Hydroxychloroquine (HCQ) Modulates Autophagy and Oxidative DNA Damage Stress in Hepatocellular Carcinoma to Overcome Sorafenib Resistance via TLR9/SOD1/hsa-miR-30a-5p/Beclin-1 Axis

Hydroxychloroquine (HCQ) Modulates Autophagy and Oxidative DNA Damage Stress in Hepatocellular Carcinoma to Overcome Sorafenib Resistance via TLR9/SOD1/hsa-miR-30a-5p/Beclin-1 Axis

Supplementing organic-complexed or inorganic Co, Cu, Mn, and Zn to beef cows during gestation: physiological and productive response of cows and their offspring until weaning

Light-Dependent Phenomena and Related Molecular Mechanisms in Giant Clam-Dinoflagellate Associations: A Review

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